BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0577000 Os04g0577000|AK073711
         (320 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0577000  Ubiquitin fusion degradation protein UFD1 fami...   615   e-176
Os02g0181800  Similar to Ubiquitin fusion degradation 1-like      387   e-108
Os01g0144500                                                      211   6e-55
Os01g0534800  Similar to PRLI-interacting factor K (Fragment)     105   5e-23

>Os04g0577000 Ubiquitin fusion degradation protein UFD1 family protein
          Length = 320

 Score =  615 bits (1585), Expect = e-176,   Method: Compositional matrix adjust.
 Identities = 298/320 (93%), Positives = 298/320 (93%)

Query: 1   MFYAGYGYHGSNFEQTYRCYPASFFDKPHLEGGDKVIMPPSALDRLASLHIEYPMLFELH 60
           MFYAGYGYHGSNFEQTYRCYPASFFDKPHLEGGDKVIMPPSALDRLASLHIEYPMLFELH
Sbjct: 1   MFYAGYGYHGSNFEQTYRCYPASFFDKPHLEGGDKVIMPPSALDRLASLHIEYPMLFELH 60

Query: 61  NDATQRISHCGVLEFVAEEGMIIMPYWMMQNMLLQEGDTVRVKNTTLPKGTYVKLQPHTT 120
           NDATQRISHCGVLEFVAEEGMIIMPYWMMQNMLLQEGDTVRVKNTTLPKGTYVKLQPHTT
Sbjct: 61  NDATQRISHCGVLEFVAEEGMIIMPYWMMQNMLLQEGDTVRVKNTTLPKGTYVKLQPHTT 120

Query: 121 DFLDISNPKAILEKTLRKLSCITTGDSIMVAYNNKQYYIDIVETKPASAVSIIETDCEVD 180
           DFLDISNPKAILEKTLRKLSCITTGDSIMVAYNNKQYYIDIVETKPASAVSIIETDCEVD
Sbjct: 121 DFLDISNPKAILEKTLRKLSCITTGDSIMVAYNNKQYYIDIVETKPASAVSIIETDCEVD 180

Query: 181 FAPPLDYKEPENPQQPSVPASEATAEDENAKVEDELKFKPFTGSGKRLDGKASKLQATEV 240
           FAPPLDYKEPENPQQPSVPASEATAEDENAKVEDELKFKPFTGSGKRLDGKASKLQATEV
Sbjct: 181 FAPPLDYKEPENPQQPSVPASEATAEDENAKVEDELKFKPFTGSGKRLDGKASKLQATEV 240

Query: 241 PXXXXXXXXXXNKRVNQETLAPASSGASNSTRQKSGKLVFGSSANNNKEPQKASVXXXXX 300
           P          NKRVNQETLAPASSGASNSTRQKSGKLVFGSSANNNKEPQKASV     
Sbjct: 241 PSASRSSPSDSNKRVNQETLAPASSGASNSTRQKSGKLVFGSSANNNKEPQKASVKDDES 300

Query: 301 XXXXXXXFQAFSGKSYSLKR 320
                  FQAFSGKSYSLKR
Sbjct: 301 PKKDEPKFQAFSGKSYSLKR 320



>Os02g0181800 Similar to Ubiquitin fusion degradation 1-like
          Length = 324

 Score =  387 bits (995), Expect = e-108,   Method: Compositional matrix adjust.
 Identities = 205/334 (61%), Positives = 234/334 (70%), Gaps = 35/334 (10%)

Query: 1   MFYAGYGYHGSNFEQTYRCYPASFFDKPHLEGGDKVIMPPSALDRLASLHIEYPMLFELH 60
           M++ GYGY GS FEQTYRCYPASF DKP LE GDK+IMPPSALDRLASLHIEYPMLFE+H
Sbjct: 10  MYFEGYGYRGSTFEQTYRCYPASFIDKPQLETGDKIIMPPSALDRLASLHIEYPMLFEVH 69

Query: 61  NDATQRISHCGVLEFVAEEGMIIMPYWMMQNMLLQEGDTVRVKNTTLPKGTYVKLQPHTT 120
           N A +R SHCGVLEF+AEEGMI MPYWMMQN+LL EGD V +KN  LPKGTYVKLQPHTT
Sbjct: 70  NAAAERTSHCGVLEFIAEEGMIYMPYWMMQNLLLTEGDMVFIKNANLPKGTYVKLQPHTT 129

Query: 121 DFLDISNPKAILEKTLRKLSCITTGDSIMVAYNNKQYYIDIVETKPASAVSIIETDCEVD 180
           DFLDISNPKAILEKTLR  SC+TTGDSIMVAYNNK+YYIDIVETKP++A+SIIETDCEVD
Sbjct: 130 DFLDISNPKAILEKTLRNYSCLTTGDSIMVAYNNKKYYIDIVETKPSNAISIIETDCEVD 189

Query: 181 FAPPLDYKEPENPQQPSVPASEATAEDENAKVEDELKFKPFTGSGKRLDGKASKLQATEV 240
           FAPPLDYKEPE  Q+ +VP S A         E+E KF PFTG G+RLDGKA K      
Sbjct: 190 FAPPLDYKEPER-QKAAVPPSTAPT--AEEAAEEEPKFIPFTGPGRRLDGKAPK------ 240

Query: 241 PXXXXXXXXXXNKRVNQETLAPASSGASNSTR-------------QKSGKLVFGSSAN-N 286
                      +K V+  + A   + A+NS +             + + KLVFG   N  
Sbjct: 241 -----------DKDVSASSPAKRQANATNSVQPSTASTSQSSSSRKTTEKLVFGPGGNRT 289

Query: 287 NKEPQKA-SVXXXXXXXXXXXXFQAFSGKSYSLK 319
           +KE +K                F AF+G+ YSLK
Sbjct: 290 SKETEKVPEKEPKEDPKKDEPKFSAFTGRKYSLK 323



>Os01g0144500 
          Length = 281

 Score =  211 bits (537), Expect = 6e-55,   Method: Compositional matrix adjust.
 Identities = 104/221 (47%), Positives = 142/221 (64%), Gaps = 10/221 (4%)

Query: 11  SNFEQTYRCYPASFFDKPHLEGGDKVIMPPSALDRLASLHIEYPMLFELHNDATQRISHC 70
           + F Q YRC P S   K + + G++V MP SALDRL  LHIEYPM F++ N  T + S+C
Sbjct: 12  ATFAQLYRCLPISLLKKENADDGNRVFMPVSALDRLGYLHIEYPMQFQIQNATTLQTSYC 71

Query: 71  GVLEFVAEEGMIIMPYWMMQNMLLQEGDTVRVKNTTLPKGTYVKLQPHTTDFLDISNPKA 130
           GVLEF A+EG I +P  MM+++ L+E D V +++T++PK T++KLQPHT+DF  +S P+ 
Sbjct: 72  GVLEFTADEGFIHIPTMMMEHLGLRENDLVLLRSTSIPKATFIKLQPHTSDFHKLSEPRY 131

Query: 131 ILEKTLRKLSCITTGDSIMVAYNNKQYYIDIVETKPASAVSIIETDCEVDFAPPLDYKEP 190
           +LE   R   C+TTG++I VA  ++ YY+D+VET+PA AV +IETDCEV+F   LD  EP
Sbjct: 132 LLEYNFRNYFCLTTGETIAVAAGDRFYYLDVVETRPADAVCVIETDCEVEFDQALDQAEP 191

Query: 191 ENPQQPSVPASEATAEDENAKVEDELKFKPFTGSGKRLDGK 231
                  V    A  E E A+         FTG   R+DGK
Sbjct: 192 AAAAAMQVDGVGA-GEPEPAR---------FTGFRMRMDGK 222



>Os01g0534800 Similar to PRLI-interacting factor K (Fragment)
          Length = 569

 Score =  105 bits (261), Expect = 5e-23,   Method: Compositional matrix adjust.
 Identities = 74/221 (33%), Positives = 109/221 (49%), Gaps = 29/221 (13%)

Query: 20  YPASFFDKPHLEGGDKVIMPPSALDRLASLHI--EYPMLFEL------------HNDATQ 65
           + A  +D P    GDK+ +PPS+   L+      + PM F L             NDA +
Sbjct: 82  FEALRYDGP----GDKIKLPPSSFKELSDEGALDKGPMYFRLSKVRDSVPGAPQDNDADE 137

Query: 66  RISHCGVLEFVAEEGMIIMPYWMMQNMLLQEGDT-----VRVKNTTLPKGTYVKLQPHTT 120
               CGVLEF A EG   +   +  N+    GD+     + V+  +LPKGTY KL+P   
Sbjct: 138 ATC-CGVLEFTAREGSAELTPHVWNNLF--RGDSPDVPLIEVRYVSLPKGTYAKLKPEGV 194

Query: 121 DFLDISNPKAILEKTLRKLSCITTGDSIMVAYNNKQYYIDIVETKPASAVSIIETDCEVD 180
            F D+ N +A+LE  LR  + ++  D ++V Y   QY + ++E KPAS+VS++ETD EVD
Sbjct: 195 GFSDLPNHRAVLETALRNHATLSENDFVVVNYGQLQYKLKVLELKPASSVSVLETDVEVD 254

Query: 181 FAPPLDYKEPENPQQPSVPASEATAEDENAKVEDELKFKPF 221
              P    +    Q   VP      E   + V +E KF+ +
Sbjct: 255 IEGPDSVLDNVENQHVLVPLETGKVE---SGVVEEGKFRYY 292



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.313    0.130    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 10,426,944
Number of extensions: 414478
Number of successful extensions: 866
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 860
Number of HSP's successfully gapped: 4
Length of query: 320
Length of database: 17,035,801
Length adjustment: 101
Effective length of query: 219
Effective length of database: 11,762,187
Effective search space: 2575918953
Effective search space used: 2575918953
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 156 (64.7 bits)