BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0531100 Os04g0531100|AK063584
         (144 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0531100  C2 calcium/lipid-binding region, CaLB domain c...   296   3e-81
Os02g0640000  C2 calcium/lipid-binding region, CaLB domain c...   244   2e-65
Os04g0682100  Eukaryotic phosphomannomutase family protein        177   2e-45
Os01g0841700  RPP17-1                                             107   3e-24

>Os04g0531100 C2 calcium/lipid-binding region, CaLB domain containing protein
          Length = 144

 Score =  296 bits (759), Expect = 3e-81,   Method: Compositional matrix adjust.
 Identities = 144/144 (100%), Positives = 144/144 (100%)

Query: 1   MVQGTLEVLLVGAKGLENTDYLCNMDPYAVLKCRSQEQKSSVASGKGSDPEWNETFMFSV 60
           MVQGTLEVLLVGAKGLENTDYLCNMDPYAVLKCRSQEQKSSVASGKGSDPEWNETFMFSV
Sbjct: 1   MVQGTLEVLLVGAKGLENTDYLCNMDPYAVLKCRSQEQKSSVASGKGSDPEWNETFMFSV 60

Query: 61  THNATELIIKLMDSDSGTDDDFVGEATISLEAIYTEGSIPPTVYNVVKEEEYRGEIKVGL 120
           THNATELIIKLMDSDSGTDDDFVGEATISLEAIYTEGSIPPTVYNVVKEEEYRGEIKVGL
Sbjct: 61  THNATELIIKLMDSDSGTDDDFVGEATISLEAIYTEGSIPPTVYNVVKEEEYRGEIKVGL 120

Query: 121 TFTPEDDRDRGLSEEDIGGWKQSS 144
           TFTPEDDRDRGLSEEDIGGWKQSS
Sbjct: 121 TFTPEDDRDRGLSEEDIGGWKQSS 144



>Os02g0640000 C2 calcium/lipid-binding region, CaLB domain containing protein
          Length = 143

 Score =  244 bits (622), Expect = 2e-65,   Method: Compositional matrix adjust.
 Identities = 116/143 (81%), Positives = 130/143 (90%), Gaps = 1/143 (0%)

Query: 1   MVQGTLEVLLVGAKGLENTDYLCNMDPYAVLKCRSQEQKSSVASGKGSDPEWNETFMFSV 60
           MV GTLEVLLVGAKGLENTDYLCNMDPYA+LKCRSQEQ+SS+ASGKGS+PEWNE F+F+V
Sbjct: 1   MVHGTLEVLLVGAKGLENTDYLCNMDPYAILKCRSQEQRSSIASGKGSNPEWNENFVFTV 60

Query: 61  THNATELIIKLMDSDSGTDDDFVGEATISLEAIYTEGSIPPTVYNVVKEEEYRGEIKVGL 120
           +  ATEL+IKL+DSD+G+ DDFVGEATI LEA+YTEGSIPPT+YNVVK+E Y GEIKVGL
Sbjct: 61  SDKATELLIKLLDSDTGSADDFVGEATIPLEAVYTEGSIPPTLYNVVKDEHYCGEIKVGL 120

Query: 121 TFTPEDDRDRGLSEEDIGGWKQS 143
           TFTPED R RGL  ED GGWKQS
Sbjct: 121 TFTPEDVRQRGLP-EDFGGWKQS 142



>Os04g0682100 Eukaryotic phosphomannomutase family protein
          Length = 145

 Score =  177 bits (450), Expect = 2e-45,   Method: Compositional matrix adjust.
 Identities = 84/138 (60%), Positives = 107/138 (77%)

Query: 1   MVQGTLEVLLVGAKGLENTDYLCNMDPYAVLKCRSQEQKSSVASGKGSDPEWNETFMFSV 60
           MV G LEVLLV AKGLE+TD+L +MDPY +L CR+QEQKSSVA G GS+PEWNETF+F+V
Sbjct: 1   MVHGKLEVLLVCAKGLEDTDFLNDMDPYVILTCRTQEQKSSVAKGAGSEPEWNETFVFTV 60

Query: 61  THNATELIIKLMDSDSGTDDDFVGEATISLEAIYTEGSIPPTVYNVVKEEEYRGEIKVGL 120
           + +  +L +K+MDSD+ + DDFVGEA I LE ++ EGS+PP V+ VVKEE+Y GEIKV L
Sbjct: 61  SDDVPQLNVKIMDSDAFSADDFVGEANIPLEPVFLEGSLPPAVHRVVKEEKYCGEIKVAL 120

Query: 121 TFTPEDDRDRGLSEEDIG 138
           TFTP  +     + E+ G
Sbjct: 121 TFTPAAETRHHHNHENEG 138



>Os01g0841700 RPP17-1
          Length = 159

 Score =  107 bits (267), Expect = 3e-24,   Method: Compositional matrix adjust.
 Identities = 63/154 (40%), Positives = 88/154 (57%), Gaps = 16/154 (10%)

Query: 4   GTLEVLLVGAKGLENTDYLC---NMDPYAVLKCRSQEQKSSVASGKGSDPEWNETFMFSV 60
           G LEV LV AKGL   D+L     +DPY V++ RSQE+KSSVA  +G +P WNE F F +
Sbjct: 5   GVLEVHLVDAKGLTGNDFLGEIGKIDPYVVVQYRSQERKSSVARDQGKNPSWNEVFKFQI 64

Query: 61  THNAT----ELIIKLMDSDSGTDDDFVGEATISLEAIYTEG------SIPPTVYNVV-KE 109
              A     +L ++LMD D+ + DDF+GEATI++  + + G       +  + + VV  +
Sbjct: 65  NSTAATGQHKLFLRLMDHDTFSRDDFLGEATINVTDLISLGMEHGTWEMSESKHRVVLAD 124

Query: 110 EEYRGEIKVGLTFTPEDDRDRGLSEEDIGGWKQS 143
           + Y GEI+V LTFT           E +GGW  S
Sbjct: 125 KTYHGEIRVSLTFTASAKAQD--HAEQVGGWAHS 156



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.310    0.131    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,194,041
Number of extensions: 214364
Number of successful extensions: 451
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 460
Number of HSP's successfully gapped: 4
Length of query: 144
Length of database: 17,035,801
Length adjustment: 91
Effective length of query: 53
Effective length of database: 12,284,327
Effective search space: 651069331
Effective search space used: 651069331
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 151 (62.8 bits)