BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0485800 Os04g0485800|AK100299
         (453 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0485800  Cyclin-like F-box domain containing protein         858   0.0  
Os08g0236800  Hypothetical protein                                132   7e-31
Os08g0428100  Cyclin-like F-box domain containing protein         104   2e-22

>Os04g0485800 Cyclin-like F-box domain containing protein
          Length = 453

 Score =  858 bits (2218), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 424/453 (93%), Positives = 424/453 (93%)

Query: 1   MALNYSSCSLTSSFLMNEDCAGMMCGCGCWSEEASPLSSGGVNSLWWDXXXXXXXXXXXX 60
           MALNYSSCSLTSSFLMNEDCAGMMCGCGCWSEEASPLSSGGVNSLWWD            
Sbjct: 1   MALNYSSCSLTSSFLMNEDCAGMMCGCGCWSEEASPLSSGGVNSLWWDELEFELELEEEE 60

Query: 61  XXDPVDLLPTDPFGMNLETTFTAAIASCIEDLTVMSSAGRFGDSRDDAVFADLSYYLNKA 120
             DPVDLLPTDPFGMNLETTFTAAIASCIEDLTVMSSAGRFGDSRDDAVFADLSYYLNKA
Sbjct: 61  EFDPVDLLPTDPFGMNLETTFTAAIASCIEDLTVMSSAGRFGDSRDDAVFADLSYYLNKA 120

Query: 121 FVLSPEFQFGGYRGVFEGPLGFGGLSAGEGDSFGFMKNPSSSGNADDSFGFVETPPTSGN 180
           FVLSPEFQFGGYRGVFEGPLGFGGLSAGEGDSFGFMKNPSSSGNADDSFGFVETPPTSGN
Sbjct: 121 FVLSPEFQFGGYRGVFEGPLGFGGLSAGEGDSFGFMKNPSSSGNADDSFGFVETPPTSGN 180

Query: 181 AALECGDAXXXXXXXXXXXXXXXMLFALDYLGLRDILSVERVCKTLHSAVRNEPLLWKSI 240
           AALECGDA               MLFALDYLGLRDILSVERVCKTLHSAVRNEPLLWKSI
Sbjct: 181 AALECGDAVEVVPVQEGGVPHEGMLFALDYLGLRDILSVERVCKTLHSAVRNEPLLWKSI 240

Query: 241 HIEGDLRQRISDAGLLHLTQKCPDTLQCLSIACCVNITDQGLKAVLESNPRLTKLSILGC 300
           HIEGDLRQRISDAGLLHLTQKCPDTLQCLSIACCVNITDQGLKAVLESNPRLTKLSILGC
Sbjct: 241 HIEGDLRQRISDAGLLHLTQKCPDTLQCLSIACCVNITDQGLKAVLESNPRLTKLSILGC 300

Query: 301 PRLTLDGLISNLKSFNTKAVFGIKHLRVGTLFSLRKEQYEELLSLLNTDKTQEVHNRGPR 360
           PRLTLDGLISNLKSFNTKAVFGIKHLRVGTLFSLRKEQYEELLSLLNTDKTQEVHNRGPR
Sbjct: 301 PRLTLDGLISNLKSFNTKAVFGIKHLRVGTLFSLRKEQYEELLSLLNTDKTQEVHNRGPR 360

Query: 361 FLHANRFLSDCNDGYALDIEMCPICQNYKLVYDCPDEGCDDRRSGNCKGCTVCILRCYEC 420
           FLHANRFLSDCNDGYALDIEMCPICQNYKLVYDCPDEGCDDRRSGNCKGCTVCILRCYEC
Sbjct: 361 FLHANRFLSDCNDGYALDIEMCPICQNYKLVYDCPDEGCDDRRSGNCKGCTVCILRCYEC 420

Query: 421 GRCVDKLAFKESFSLDWVCPNCQEKKDLSPPMK 453
           GRCVDKLAFKESFSLDWVCPNCQEKKDLSPPMK
Sbjct: 421 GRCVDKLAFKESFSLDWVCPNCQEKKDLSPPMK 453



>Os08g0236800 Hypothetical protein
          Length = 71

 Score =  132 bits (331), Expect = 7e-31,   Method: Compositional matrix adjust.
 Identities = 64/67 (95%), Positives = 65/67 (97%)

Query: 295 LSILGCPRLTLDGLISNLKSFNTKAVFGIKHLRVGTLFSLRKEQYEELLSLLNTDKTQEV 354
           LSILGCPRLTLDGLISNLKSFNT AVFGIKHLRVGTLFSLRKEQYEELLSLLNTDKTQEV
Sbjct: 2   LSILGCPRLTLDGLISNLKSFNTNAVFGIKHLRVGTLFSLRKEQYEELLSLLNTDKTQEV 61

Query: 355 HNRGPRF 361
           HNRGP+ 
Sbjct: 62  HNRGPKI 68



>Os08g0428100 Cyclin-like F-box domain containing protein
          Length = 338

 Score =  104 bits (259), Expect = 2e-22,   Method: Compositional matrix adjust.
 Identities = 71/256 (27%), Positives = 124/256 (48%), Gaps = 29/256 (11%)

Query: 214 RDILSVERVCKTLHSAVRNEPLLWKSIHIEGD-LRQRISDAGLLHLTQKCPDTLQCLSIA 272
           R++LS    C+ L  AV  +PLLW+ + +    L +R++D  LL LT +   TL+ L + 
Sbjct: 40  RELLSCAGACRRLRDAVAGDPLLWRRVAVAAPPLARRLTDEALLALTGRAGGTLRSLRLL 99

Query: 273 CCVNITDQGLKAVLESNPRLT-----------------------KLSILGCPRLTLDGLI 309
            C  ++D GL  V+E NP +T                       KL +  C  LT +G++
Sbjct: 100 GCTRVSDAGLLRVVERNPGITEILSMESSTLLKDTMISTRAKIYKLYMPRCTGLTAEGVV 159

Query: 310 SNLKSFNTKAVFGIKHLRVGTLFSLRKEQYEELLSLLNTDKTQEVHNRGPRFLHANRFLS 369
             ++    +    +  +R+  +  + K   + + S +     QE  ++   + H    + 
Sbjct: 160 KIVQVL-YECKGNLNRIRLHGICRMTKHHLDAISSAMCKGNQQE-DDQSLFYSHRVHEVL 217

Query: 370 DCNDGYALDIEMCPICQNYKLVYDCPDEGCDDRRS-GNCKGCTVCILRCYECGRCVD--K 426
           + ND   +D+++CP+C N +LV+DC  +GC  + S   C+GC  C+ RC  CG C+D  +
Sbjct: 218 NTNDERRIDVDVCPMCTNVRLVFDCTRDGCRRKDSWAQCRGCFFCVARCETCGGCIDLEE 277

Query: 427 LAFKESFSLDWVCPNC 442
           L+  E    D++C  C
Sbjct: 278 LSETELACSDFLCMEC 293



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.138    0.440 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 15,733,166
Number of extensions: 653187
Number of successful extensions: 1711
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 1706
Number of HSP's successfully gapped: 3
Length of query: 453
Length of database: 17,035,801
Length adjustment: 104
Effective length of query: 349
Effective length of database: 11,605,545
Effective search space: 4050335205
Effective search space used: 4050335205
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 158 (65.5 bits)