BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0442800 Os04g0442800|AK121065
         (140 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0442800  Similar to Phragmoplast-associated kinesin-rel...   245   7e-66
Os02g0489800  Kinesin, motor region domain containing protein     106   6e-24
Os03g0587200  Kinesin, motor region domain containing protein      68   2e-12
Os12g0129600                                                       68   2e-12
Os11g0581000                                                       66   9e-12

>Os04g0442800 Similar to Phragmoplast-associated kinesin-related protein 1
           (Fragment)
          Length = 140

 Score =  245 bits (626), Expect = 7e-66,   Method: Compositional matrix adjust.
 Identities = 126/140 (90%), Positives = 126/140 (90%)

Query: 1   SRRLSAPCSCGSAAGDDGSVQIXXXXXXXXXXXXXXFCTCGPYTAPCRLLLLQIGEDVRT 60
           SRRLSAPCSCGSAAGDDGSVQI              FCTCGPYTAPCRLLLLQIGEDVRT
Sbjct: 1   SRRLSAPCSCGSAAGDDGSVQIRRCRPWSRPPCRHRFCTCGPYTAPCRLLLLQIGEDVRT 60

Query: 61  ACVYVESLTKELVFTTKDVTQLLVKGLSNRRTGATSANADSSRSHCVFTCVSKSESKVVL 120
           ACVYVESLTKELVFTTKDVTQLLVKGLSNRRTGATSANADSSRSHCVFTCVSKSESKVVL
Sbjct: 61  ACVYVESLTKELVFTTKDVTQLLVKGLSNRRTGATSANADSSRSHCVFTCVSKSESKVVL 120

Query: 121 LIILSCTFNFYLVMSFFLRS 140
           LIILSCTFNFYLVMSFFLRS
Sbjct: 121 LIILSCTFNFYLVMSFFLRS 140



>Os02g0489800 Kinesin, motor region domain containing protein
          Length = 1055

 Score =  106 bits (264), Expect = 6e-24,   Method: Composition-based stats.
 Identities = 53/70 (75%), Positives = 58/70 (82%)

Query: 48  RLLLLQIGEDVRTACVYVESLTKELVFTTKDVTQLLVKGLSNRRTGATSANADSSRSHCV 107
           RL+  QI EDV T+ VYVESLTKE VFT  DVTQLL KGL+NRRT AT+ANA+SSRSHCV
Sbjct: 264 RLVHFQIREDVGTSSVYVESLTKESVFTINDVTQLLEKGLANRRTEATTANAESSRSHCV 323

Query: 108 FTCVSKSESK 117
           FTC  KSESK
Sbjct: 324 FTCFIKSESK 333



>Os03g0587200 Kinesin, motor region domain containing protein
          Length = 1129

 Score = 68.2 bits (165), Expect = 2e-12,   Method: Compositional matrix adjust.
 Identities = 35/66 (53%), Positives = 47/66 (71%), Gaps = 1/66 (1%)

Query: 52  LQIGEDVRTACVYVESLTKELVFTTKDVTQLLVKGLSNRRTGATSANADSSRSHCVFTCV 111
           LQI E+     ++VE+LT E V T +DV Q+L+KGLSNR+ G TS N  SSRSH +F+CV
Sbjct: 254 LQIRENAGNG-IHVENLTDEYVSTVEDVNQILMKGLSNRKVGTTSMNLKSSRSHVIFSCV 312

Query: 112 SKSESK 117
            ++ SK
Sbjct: 313 IEAWSK 318



>Os12g0129600 
          Length = 166

 Score = 68.2 bits (165), Expect = 2e-12,   Method: Compositional matrix adjust.
 Identities = 35/44 (79%), Positives = 37/44 (84%)

Query: 45  APCRLLLLQIGEDVRTACVYVESLTKELVFTTKDVTQLLVKGLS 88
           A C  L   I EDVRTACVYVESLTKELVFTTKDVTQLLVKG++
Sbjct: 116 AGCIFLKTTIREDVRTACVYVESLTKELVFTTKDVTQLLVKGIT 159



>Os11g0581000 
          Length = 300

 Score = 65.9 bits (159), Expect = 9e-12,   Method: Compositional matrix adjust.
 Identities = 36/68 (52%), Positives = 46/68 (67%), Gaps = 5/68 (7%)

Query: 52  LQIGEDVRTAC--VYVESLTKELVFTTKDVTQLLVKGLSNRRTGATSANADSSRSHCVFT 109
            QI E+   AC  V+VE+LT E   T +DV Q+L+KGL NR+ G TS N  SSRSH +FT
Sbjct: 198 FQIREN---ACNGVHVENLTDEYESTVEDVNQILMKGLPNRKVGTTSMNLKSSRSHIIFT 254

Query: 110 CVSKSESK 117
           CV ++ SK
Sbjct: 255 CVIEAWSK 262



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.325    0.134    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,620,009
Number of extensions: 101080
Number of successful extensions: 349
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 349
Number of HSP's successfully gapped: 5
Length of query: 140
Length of database: 17,035,801
Length adjustment: 91
Effective length of query: 49
Effective length of database: 12,284,327
Effective search space: 601932023
Effective search space used: 601932023
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 150 (62.4 bits)