BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0781400 Os03g0781400|AK071970
         (299 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0781400  Ribose 5-phosphate isomerase family protein         472   e-133
Os07g0176900  Similar to Ribose-5-phosphate isomerase precur...    97   1e-20
Os04g0306400  Ribose 5-phosphate isomerase family protein          77   1e-14

>Os03g0781400 Ribose 5-phosphate isomerase family protein
          Length = 299

 Score =  472 bits (1215), Expect = e-133,   Method: Compositional matrix adjust.
 Identities = 240/240 (100%), Positives = 240/240 (100%)

Query: 60  KLTVDRFVESGMVVGLGSGPASGLAIQYLGTRLRRGSLTGILGIPSSTISASEAVKAGIQ 119
           KLTVDRFVESGMVVGLGSGPASGLAIQYLGTRLRRGSLTGILGIPSSTISASEAVKAGIQ
Sbjct: 60  KLTVDRFVESGMVVGLGSGPASGLAIQYLGTRLRRGSLTGILGIPSSTISASEAVKAGIQ 119

Query: 120 VSSYEEGTQIDFAFTDADIIEEDTMTAVIGRRKTESGEPSFMVEKGIVKSADKLAFIIGH 179
           VSSYEEGTQIDFAFTDADIIEEDTMTAVIGRRKTESGEPSFMVEKGIVKSADKLAFIIGH
Sbjct: 120 VSSYEEGTQIDFAFTDADIIEEDTMTAVIGRRKTESGEPSFMVEKGIVKSADKLAFIIGH 179

Query: 180 EKYVKGIEGSIPVLVKSANWIDTAEEIDDLFLGDAEVWRRPSIGTAGPLGGDFPLVTKEG 239
           EKYVKGIEGSIPVLVKSANWIDTAEEIDDLFLGDAEVWRRPSIGTAGPLGGDFPLVTKEG
Sbjct: 180 EKYVKGIEGSIPVLVKSANWIDTAEEIDDLFLGDAEVWRRPSIGTAGPLGGDFPLVTKEG 239

Query: 240 HHVLDVIFTTPIPDLGKVAESLEKIAGVVDHGIVSSIPSYVVVALNGEVQVLDEKSSVIP 299
           HHVLDVIFTTPIPDLGKVAESLEKIAGVVDHGIVSSIPSYVVVALNGEVQVLDEKSSVIP
Sbjct: 240 HHVLDVIFTTPIPDLGKVAESLEKIAGVVDHGIVSSIPSYVVVALNGEVQVLDEKSSVIP 299



>Os07g0176900 Similar to Ribose-5-phosphate isomerase precursor (EC 5.3.1.6)
          Length = 280

 Score = 97.4 bits (241), Expect = 1e-20,   Method: Compositional matrix adjust.
 Identities = 67/220 (30%), Positives = 112/220 (50%), Gaps = 16/220 (7%)

Query: 67  VESGMVVGLGSGPASGLAIQYLGTRLRRGSLTGILGIPSSTISASEAVKAGIQVSSYEEG 126
           VESGMV+GLG+G  +  A+  +G  L  G L+GI+G+P+S  +  +A   GI +S+ ++ 
Sbjct: 64  VESGMVLGLGTGSTAAFAVAEIGALLASGKLSGIVGVPTSKRTFEQAQSLGIPLSTLDDH 123

Query: 127 TQIDFAFTDADIIEEDTMTAVIGRRKTESGEPSFMVEKGIVKSADKLAFIIGHEKYVKGI 186
            +ID A   AD ++ D +  V GR        + + EK +  ++DK   ++   K V G+
Sbjct: 124 PRIDLAIDGADEVDPD-LNLVKGR------GGALLREKMVEAASDKFIVVVDETKLVTGL 176

Query: 187 EGS---IPVLVKSANWIDTAEEIDDLFLGDAEVWRRPSIGTAGPLGGDFPLVTKEGHHVL 243
            GS   +PV V    W      + DLF  D     +  +   G      P VT   ++++
Sbjct: 177 GGSGLAMPVEVVQFCWKYNQVRLQDLF-NDEGCEAKLRLDEGGK-----PYVTDNSNYIV 230

Query: 244 DVIFTTPIPDLGKVAESLEKIAGVVDHGIVSSIPSYVVVA 283
           D+ F TPI D     + +  + GVV+HG+   + + V++A
Sbjct: 231 DLYFKTPIKDALAAGKEISALEGVVEHGLFLDMATSVIIA 270



>Os04g0306400 Ribose 5-phosphate isomerase family protein
          Length = 275

 Score = 77.4 bits (189), Expect = 1e-14,   Method: Compositional matrix adjust.
 Identities = 69/234 (29%), Positives = 114/234 (48%), Gaps = 12/234 (5%)

Query: 66  FVESGMVVGLGSGPASGLAIQYLGTRLRRGSLTGILGIPSSTISASEAVKAGIQVSSYEE 125
            VE GM +GLG+G  +  A+  LG  LR G L  + G+P+S  + + A + GI +    E
Sbjct: 48  MVEPGMTLGLGTGSTAAHALDRLGDLLRSGELAAVAGVPTSLKTEAHAARVGIPMLPLGE 107

Query: 126 GTQIDFAFTDADIIEEDTMTAVIGRRKTESGEPSFMVEKGIVKSADKLAFIIGHEKYVK- 184
              ID +   AD ++ + +  V GR        S + EK I  S  +   I+   K V  
Sbjct: 108 AGGIDLSIDGADEVDPE-LNLVKGRGG------SLLREKMIEGSGGRFVVIVDESKLVPR 160

Query: 185 -GIEGSIPVLVKSANWIDTAEEIDDLFLG-DAEVWRRPSIGTAGPLGGDFPLVTKEGHHV 242
            G  G++PV V       T   +  +F G      R  ++ +    G +   VT  G+++
Sbjct: 161 LGCTGAVPVEVVPFGCDHTLGLVRKVFDGLPGFSARLRTVASKDGEGKEEMFVTDNGNYI 220

Query: 243 LDVIFTTPIP-DLGKVAESLEKIAGVVDHGIVSSIPSYVVVA-LNGEVQVLDEK 294
           +++ F   I  DL ++++ L +I GVV+HG+   + + VVVA  +G V +L +K
Sbjct: 221 VEMFFEDGIRGDLNEISDRLLRITGVVEHGMFLGMATSVVVAKKDGTVALLHKK 274



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.316    0.137    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 8,762,094
Number of extensions: 353398
Number of successful extensions: 779
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 776
Number of HSP's successfully gapped: 3
Length of query: 299
Length of database: 17,035,801
Length adjustment: 100
Effective length of query: 199
Effective length of database: 11,814,401
Effective search space: 2351065799
Effective search space used: 2351065799
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 156 (64.7 bits)