BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0752300 Os03g0752300|AK069303
         (347 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0752300  Similar to K+ channel protein                       657   0.0  
Os07g0108800  Similar to Calcium-activated outward-rectifyin...   417   e-117
Os01g0696100  K+ channel, two pore family protein                 157   1e-38
Os09g0299400  K+ channel, two pore family protein                 146   2e-35

>Os03g0752300 Similar to K+ channel protein
          Length = 347

 Score =  657 bits (1695), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 332/347 (95%), Positives = 332/347 (95%)

Query: 1   MDDNSIQQSLLADNPNVLQRKPSEGVNRFRRCRSTPSTDPLQGPPEKGSSVKAKELFKEM 60
           MDDNSIQQSLLADNPNVLQRKPSEGVNRFRRCRSTPSTDPLQGPPEKGSSVKAKELFKEM
Sbjct: 1   MDDNSIQQSLLADNPNVLQRKPSEGVNRFRRCRSTPSTDPLQGPPEKGSSVKAKELFKEM 60

Query: 61  RPSFRXXXXXXXXXXXXXXXAFYAVMDEISGKRTNRVLDALYFCVVTMTTVGYGDLVPNN 120
           RPSFR               AFYAVMDEISGKRTNRVLDALYFCVVTMTTVGYGDLVPNN
Sbjct: 61  RPSFRLVGLLLFIYLLVGVLAFYAVMDEISGKRTNRVLDALYFCVVTMTTVGYGDLVPNN 120

Query: 121 DTTKLLACAFVFMGMAVVALFVSKVADYLVEKQEVLFFKALHTNLKGGETKMLRAIETNR 180
           DTTKLLACAFVFMGMAVVALFVSKVADYLVEKQEVLFFKALHTNLKGGETKMLRAIETNR
Sbjct: 121 DTTKLLACAFVFMGMAVVALFVSKVADYLVEKQEVLFFKALHTNLKGGETKMLRAIETNR 180

Query: 181 IKYKFYTNALLLVLSIISGTVFLWKVEKLSLVDSFYCVCATITTLGYGDKSFSSKLGRVF 240
           IKYKFYTNALLLVLSIISGTVFLWKVEKLSLVDSFYCVCATITTLGYGDKSFSSKLGRVF
Sbjct: 181 IKYKFYTNALLLVLSIISGTVFLWKVEKLSLVDSFYCVCATITTLGYGDKSFSSKLGRVF 240

Query: 241 AVFWIITSTIIMAQFFMYLAEIYTERRQKMLANWVLTRKMTKMDLEAADLDDDRQVGAAE 300
           AVFWIITSTIIMAQFFMYLAEIYTERRQKMLANWVLTRKMTKMDLEAADLDDDRQVGAAE
Sbjct: 241 AVFWIITSTIIMAQFFMYLAEIYTERRQKMLANWVLTRKMTKMDLEAADLDDDRQVGAAE 300

Query: 301 FVVYKLKELGKINQEEISSFLEEFEKLDVDHSGTLSPYDLTLAQSAQ 347
           FVVYKLKELGKINQEEISSFLEEFEKLDVDHSGTLSPYDLTLAQSAQ
Sbjct: 301 FVVYKLKELGKINQEEISSFLEEFEKLDVDHSGTLSPYDLTLAQSAQ 347



>Os07g0108800 Similar to Calcium-activated outward-rectifying potassium channel 1
           (AtKCO1)
          Length = 349

 Score =  417 bits (1071), Expect = e-117,   Method: Compositional matrix adjust.
 Identities = 207/344 (60%), Positives = 248/344 (72%), Gaps = 16/344 (4%)

Query: 7   QQSLLADNPNVLQRKPSEGV-NRFRRCRSTPSTDPLQGPPEKGSSVKAKELFKEM----- 60
           QQ LL D  +  Q+ P EG   RFRRCR+ PS++P   P +K +S  A    K +     
Sbjct: 6   QQPLLHDGGD--QKPPPEGAARRFRRCRTAPSSEP--PPTDKDNSSAADAPPKTLFTGGG 61

Query: 61  RPSFRXXXXXXXXXXXXXXXAFYAVMDEISGKRTNRVLDALYFCVVTMTTVGYGDLVPNN 120
           RPSFR               AFY  MD +SG RT R LDALYFCVVTMTTVGYGDLVP +
Sbjct: 62  RPSFRLVGLLLVAYLLLGTIAFYLAMDHMSGTRTTRALDALYFCVVTMTTVGYGDLVPAS 121

Query: 121 DTTKLLACAFVFMGMAVVALFVSKVADYLVEKQEVLFFKALHTNLKGGETKMLRAIETNR 180
           D  KLLACAFVF G+AVV  F+SK ADYLVEKQE L F+ALH++       M+RA+E N+
Sbjct: 122 DAAKLLACAFVFAGVAVVGTFLSKAADYLVEKQEALLFRALHSH------TMVRAMEMNK 175

Query: 181 IKYKFYTNALLLVLSIISGTVFLWKVEKLSLVDSFYCVCATITTLGYGDKSFSSKLGRVF 240
           ++YK YT  LLLV ++ SGTV LWKVE +  VD+FYCVCAT+TTLGYGD+SFSS+ GR F
Sbjct: 176 VRYKLYTAGLLLVAAVASGTVVLWKVEGMRAVDAFYCVCATVTTLGYGDRSFSSEGGRAF 235

Query: 241 AVFWIITSTIIMAQFFMYLAEIYTERRQKMLANWVLTRKMTKMDLEAADLDDDRQVGAAE 300
           AV WI  ST+++A FF+Y AE+YTERRQ+ LA WVL R+ T MDLEAADLD D +VGAA+
Sbjct: 236 AVAWITVSTVVVALFFLYAAELYTERRQRELARWVLRRRTTNMDLEAADLDGDHRVGAAD 295

Query: 301 FVVYKLKELGKINQEEISSFLEEFEKLDVDHSGTLSPYDLTLAQ 344
           FV+YKLKELGKI+QE+IS FL+EF+ LD DHSGTLSP DL  AQ
Sbjct: 296 FVLYKLKELGKISQEDISEFLDEFDNLDADHSGTLSPADLAAAQ 339



>Os01g0696100 K+ channel, two pore family protein
          Length = 384

 Score =  157 bits (396), Expect = 1e-38,   Method: Compositional matrix adjust.
 Identities = 83/257 (32%), Positives = 155/257 (60%), Gaps = 12/257 (4%)

Query: 91  GKRTNRVLDALYFCVVTMTTVGYGDLVPNNDTTKLLACAFVFMGMAVVALFVSKVADYLV 150
           G+ T +++D LYF ++++ T+GYGD+VP    TK+  C F+ +G+  V + ++++   ++
Sbjct: 129 GRSTLKLVDGLYFTIISLCTIGYGDIVPCTTFTKVFTCLFLLIGVRFVDIVLNELLTNVL 188

Query: 151 EKQEVLFFKALHTNL--KGGETKMLRAIETNRIKYKFYTNALLLVLSIISGTV-----FL 203
           +KQ  +    +  N   +  +T M+ A E  R + +     +LL L ++ GT+      +
Sbjct: 189 DKQRTVLLSTMDDNKLNRVFDTYMIDA-EKKRSRGRM---KVLLALGVVVGTISICTIIV 244

Query: 204 WKVEKLSLVDSFYCVCATITTLGYGDKSFSSKLGRVFAVFWIITSTIIMAQFFMYLAEIY 263
            +VE L+ +DSFY    ++TT+GYGD  FS+  GR+ A   ++ ST+ +A+ F++L ++ 
Sbjct: 245 HEVEGLNWIDSFYLSVISVTTVGYGDYGFSTPAGRLSATVCLLVSTLAVAKAFLFLTDLR 304

Query: 264 TERRQKMLANWVLTRKMTKMDLEAADLDDDRQVGAAEFVVYKLKELGKINQEEISSFLEE 323
            +RR +    W+L +KM    L AADLD D  V  ++F++YKLKE+GKI+ ++I+   ++
Sbjct: 305 MDRRNRKTTKWILQKKMDNEPL-AADLDHDASVSKSDFLIYKLKEIGKIDDKDIAMISDQ 363

Query: 324 FEKLDVDHSGTLSPYDL 340
           F++L +   G ++  D+
Sbjct: 364 FDQLGLAKCGKITLADI 380



>Os09g0299400 K+ channel, two pore family protein
          Length = 413

 Score =  146 bits (369), Expect = 2e-35,   Method: Compositional matrix adjust.
 Identities = 75/211 (35%), Positives = 125/211 (59%), Gaps = 4/211 (1%)

Query: 90  SGKRTNRVLDALYFCVVTMTTVGYGDLVPNNDTTKLLACAFVFMGMAVVALFVSKVADYL 149
           S   T+ V DALYFC+VT+ T+GYGD+ P     KL + +FV +G   V + +S +  Y+
Sbjct: 183 SAGPTHPVADALYFCIVTLCTIGYGDITPATPAAKLFSISFVLIGFGFVDILLSGMVSYV 242

Query: 150 VEKQEVLFFKALHT--NLKGGETKMLRAIETNRIKYKFYTNALLLVLSIISGT--VFLWK 205
           ++ QE L   AL    +++      +  ++  R++ +      L V++I  G     L +
Sbjct: 243 LDLQEHLLITALKNPRSVRKHRHNYIFDLKKGRMRVRMKVALALTVVAICVGVGAAVLKR 302

Query: 206 VEKLSLVDSFYCVCATITTLGYGDKSFSSKLGRVFAVFWIITSTIIMAQFFMYLAEIYTE 265
           VE L  +D+ Y    ++TT+GYGD +F +  GR+FA  W++ ST+ +A+ F+YLAE+  +
Sbjct: 303 VENLGWLDAVYLAVMSVTTVGYGDHAFRTLAGRLFASAWLLVSTLAVARAFLYLAEMRID 362

Query: 266 RRQKMLANWVLTRKMTKMDLEAADLDDDRQV 296
           +R + +ANWVL+R MT  +  AAD+D++  V
Sbjct: 363 KRHRAMANWVLSRDMTVSEFLAADIDNNGYV 393



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.322    0.135    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 9,914,148
Number of extensions: 350185
Number of successful extensions: 978
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 965
Number of HSP's successfully gapped: 4
Length of query: 347
Length of database: 17,035,801
Length adjustment: 102
Effective length of query: 245
Effective length of database: 11,709,973
Effective search space: 2868943385
Effective search space used: 2868943385
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 156 (64.7 bits)