BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0711500 Os03g0711500|AK058652
         (504 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0711500  Similar to Coatomer alpha subunit                   879   0.0  
Os03g0711400  Similar to Coatomer alpha subunit                   868   0.0  
Os09g0127800  Similar to Coatomer alpha subunit                   856   0.0  
AK110077                                                          147   2e-35

>Os03g0711500 Similar to Coatomer alpha subunit
          Length = 504

 Score =  879 bits (2270), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 436/504 (86%), Positives = 436/504 (86%)

Query: 1   MDKVGFMCKIAGQNNNLMGQFHNALYLGDAMKRVEILENAGQLPLAYITATTHGLTEIAD 60
           MDKVGFMCKIAGQNNNLMGQFHNALYLGDAMKRVEILENAGQLPLAYITATTHGLTEIAD
Sbjct: 1   MDKVGFMCKIAGQNNNLMGQFHNALYLGDAMKRVEILENAGQLPLAYITATTHGLTEIAD 60

Query: 61  RLAAELGENIPSLPEGKARSLLIPPAPLTASGDWPLLRVMRGIFEGGLDATGKXXXXXXX 120
           RLAAELGENIPSLPEGKARSLLIPPAPLTASGDWPLLRVMRGIFEGGLDATGK       
Sbjct: 61  RLAAELGENIPSLPEGKARSLLIPPAPLTASGDWPLLRVMRGIFEGGLDATGKAELEEDD 120

Query: 121 XXXXXXXXXXXXXIVDASEAMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTPK 180
                        IVDASEAM                                    TPK
Sbjct: 121 EAAGADWGDEGLDIVDASEAMANGGDGFDAEEGEANEEDGEEGGWDLEDLELLPEAETPK 180

Query: 181 NAGNARSAVFVAPPPGMPVSLIWTQKSSLAGEHAAAGNFDTAMRLLSRQLGIKNFAPLKP 240
           NAGNARSAVFVAPPPGMPVSLIWTQKSSLAGEHAAAGNFDTAMRLLSRQLGIKNFAPLKP
Sbjct: 181 NAGNARSAVFVAPPPGMPVSLIWTQKSSLAGEHAAAGNFDTAMRLLSRQLGIKNFAPLKP 240

Query: 241 LFLDLHMGSHSYLHALATAPIIPVAVEKGWSESASPNVRGPPALVFTFPQMEDRLKAAYK 300
           LFLDLHMGSHSYLHALATAPIIPVAVEKGWSESASPNVRGPPALVFTFPQMEDRLKAAYK
Sbjct: 241 LFLDLHMGSHSYLHALATAPIIPVAVEKGWSESASPNVRGPPALVFTFPQMEDRLKAAYK 300

Query: 301 ATTDGKFPEALRQFLSILHTIPLIVVDSRREVDEVKELIEIVREYVLGLRMELKRKELRD 360
           ATTDGKFPEALRQFLSILHTIPLIVVDSRREVDEVKELIEIVREYVLGLRMELKRKELRD
Sbjct: 301 ATTDGKFPEALRQFLSILHTIPLIVVDSRREVDEVKELIEIVREYVLGLRMELKRKELRD 360

Query: 361 DVNRQQELAAYFTNCKLQRVHMRLVLGSAMGLCYKQKNFATAEHFARMLLENNPNEXXXX 420
           DVNRQQELAAYFTNCKLQRVHMRLVLGSAMGLCYKQKNFATAEHFARMLLENNPNE    
Sbjct: 361 DVNRQQELAAYFTNCKLQRVHMRLVLGSAMGLCYKQKNFATAEHFARMLLENNPNEAQAR 420

Query: 421 XXXXXXXXCSGKKDSSELNYDYRNPFVVCGATYVPIYRGQKDVSCPYCGSRFVPSIEGQL 480
                   CSGKKDSSELNYDYRNPFVVCGATYVPIYRGQKDVSCPYCGSRFVPSIEGQL
Sbjct: 421 RARQVQQQCSGKKDSSELNYDYRNPFVVCGATYVPIYRGQKDVSCPYCGSRFVPSIEGQL 480

Query: 481 CTICELAVVGADASGLVCSPTQLR 504
           CTICELAVVGADASGLVCSPTQLR
Sbjct: 481 CTICELAVVGADASGLVCSPTQLR 504



>Os03g0711400 Similar to Coatomer alpha subunit
          Length = 1218

 Score =  868 bits (2242), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 428/504 (84%), Positives = 433/504 (85%)

Query: 1    MDKVGFMCKIAGQNNNLMGQFHNALYLGDAMKRVEILENAGQLPLAYITATTHGLTEIAD 60
            MDKVGFMCKIAGQNNNLMGQFHNALYLGDA+KRVEILENAGQLPLAYITATTHGLTEIAD
Sbjct: 715  MDKVGFMCKIAGQNNNLMGQFHNALYLGDALKRVEILENAGQLPLAYITATTHGLTEIAD 774

Query: 61   RLAAELGENIPSLPEGKARSLLIPPAPLTASGDWPLLRVMRGIFEGGLDATGKXXXXXXX 120
            RLAAELGENIPSLPEGKARSLLIPPAPLTASGDWPLLRVM GIFEGGLDATGK       
Sbjct: 775  RLAAELGENIPSLPEGKARSLLIPPAPLTASGDWPLLRVMHGIFEGGLDATGKAELEEDD 834

Query: 121  XXXXXXXXXXXXXIVDASEAMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTPK 180
                         +VDASEAM                                    TPK
Sbjct: 835  EAAGADWGDEDLDMVDASEAMANGGDGFDAEEGEANEEDGEEGGWDLEDLELPPEAETPK 894

Query: 181  NAGNARSAVFVAPPPGMPVSLIWTQKSSLAGEHAAAGNFDTAMRLLSRQLGIKNFAPLKP 240
            NAGNARSAVFVAPPPGMPVSLIWTQKSSLAGEHAAAGNFDTAMRLLSRQLGIKNFAPLKP
Sbjct: 895  NAGNARSAVFVAPPPGMPVSLIWTQKSSLAGEHAAAGNFDTAMRLLSRQLGIKNFAPLKP 954

Query: 241  LFLDLHMGSHSYLHALATAPIIPVAVEKGWSESASPNVRGPPALVFTFPQMEDRLKAAYK 300
            LF+DLHMGSHSYL ALATAPIIP+AVEKGWSESASPNVRGPPALVFTFPQMEDRLKAAYK
Sbjct: 955  LFVDLHMGSHSYLRALATAPIIPIAVEKGWSESASPNVRGPPALVFTFPQMEDRLKAAYK 1014

Query: 301  ATTDGKFPEALRQFLSILHTIPLIVVDSRREVDEVKELIEIVREYVLGLRMELKRKELRD 360
            ATTDGKFPEALRQFLSILHTIPLIVVDSRREVDEVKELIEIVREYVLGLRMELKRKELRD
Sbjct: 1015 ATTDGKFPEALRQFLSILHTIPLIVVDSRREVDEVKELIEIVREYVLGLRMELKRKELRD 1074

Query: 361  DVNRQQELAAYFTNCKLQRVHMRLVLGSAMGLCYKQKNFATAEHFARMLLENNPNEXXXX 420
            DVNRQQELAAYFTNCKLQRVHMRLVLGSAMGLCYKQKNFATAEHFARMLLENNPNE    
Sbjct: 1075 DVNRQQELAAYFTNCKLQRVHMRLVLGSAMGLCYKQKNFATAEHFARMLLENNPNEAQAR 1134

Query: 421  XXXXXXXXCSGKKDSSELNYDYRNPFVVCGATYVPIYRGQKDVSCPYCGSRFVPSIEGQL 480
                    CSGKKDSSELNYDYRNPFVVCGATYVPIYRGQKDVSCPYCGSRFVPSIEGQL
Sbjct: 1135 RARQVQQQCSGKKDSSELNYDYRNPFVVCGATYVPIYRGQKDVSCPYCGSRFVPSIEGQL 1194

Query: 481  CTICELAVVGADASGLVCSPTQLR 504
            CTICELAVVGADASGL+CSPTQ R
Sbjct: 1195 CTICELAVVGADASGLLCSPTQSR 1218



>Os09g0127800 Similar to Coatomer alpha subunit
          Length = 1218

 Score =  856 bits (2212), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 424/504 (84%), Positives = 428/504 (84%)

Query: 1    MDKVGFMCKIAGQNNNLMGQFHNALYLGDAMKRVEILENAGQLPLAYITATTHGLTEIAD 60
            MDKVGFMCKIAGQNNNLMGQFHNALYLGDAMKRVEILENAGQLPLAYITA THGLTEIAD
Sbjct: 715  MDKVGFMCKIAGQNNNLMGQFHNALYLGDAMKRVEILENAGQLPLAYITAATHGLTEIAD 774

Query: 61   RLAAELGENIPSLPEGKARSLLIPPAPLTASGDWPLLRVMRGIFEGGLDATGKXXXXXXX 120
            RLAAELGENIPSLPEGK RSLLIPPAPLTASGDWPLLRVMRGIFEGGLDATGK       
Sbjct: 775  RLAAELGENIPSLPEGKTRSLLIPPAPLTASGDWPLLRVMRGIFEGGLDATGKAELEEDD 834

Query: 121  XXXXXXXXXXXXXIVDASEAMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTPK 180
                         +VDASEAM                                    TPK
Sbjct: 835  EAAGADWGDEDLDMVDASEAMANGGDGFDAEEGEANEEDGEEGGWDLEDLELPPEAETPK 894

Query: 181  NAGNARSAVFVAPPPGMPVSLIWTQKSSLAGEHAAAGNFDTAMRLLSRQLGIKNFAPLKP 240
            NAGNA S VFVAPPPGMPVS IWTQKSSLAGEHAAAGNFDTAMRLLSRQLGIKNFAPLKP
Sbjct: 895  NAGNALSVVFVAPPPGMPVSQIWTQKSSLAGEHAAAGNFDTAMRLLSRQLGIKNFAPLKP 954

Query: 241  LFLDLHMGSHSYLHALATAPIIPVAVEKGWSESASPNVRGPPALVFTFPQMEDRLKAAYK 300
            LFLDLHMGSHSYL ALATAPIIPVAVEKGWSESASPNVRGPPALVFTF QMEDRLKAAYK
Sbjct: 955  LFLDLHMGSHSYLRALATAPIIPVAVEKGWSESASPNVRGPPALVFTFSQMEDRLKAAYK 1014

Query: 301  ATTDGKFPEALRQFLSILHTIPLIVVDSRREVDEVKELIEIVREYVLGLRMELKRKELRD 360
            ATT+GKFPEALRQFL+ILHTIPLIVVDSRREVDEVKELIEIVREYVLGLRMELKRKELRD
Sbjct: 1015 ATTEGKFPEALRQFLNILHTIPLIVVDSRREVDEVKELIEIVREYVLGLRMELKRKELRD 1074

Query: 361  DVNRQQELAAYFTNCKLQRVHMRLVLGSAMGLCYKQKNFATAEHFARMLLENNPNEXXXX 420
            DVNRQQELAAYFTNCKLQRVHMRLVLGSAMGLCYKQKNFATAEHFARMLLENNPNE    
Sbjct: 1075 DVNRQQELAAYFTNCKLQRVHMRLVLGSAMGLCYKQKNFATAEHFARMLLENNPNESQAK 1134

Query: 421  XXXXXXXXCSGKKDSSELNYDYRNPFVVCGATYVPIYRGQKDVSCPYCGSRFVPSIEGQL 480
                    CSGKKDS ELNYDYRNPFVVCGATYVPIYRGQKDVSCPYCGSRFVPSIEGQL
Sbjct: 1135 RARQVQQQCSGKKDSCELNYDYRNPFVVCGATYVPIYRGQKDVSCPYCGSRFVPSIEGQL 1194

Query: 481  CTICELAVVGADASGLVCSPTQLR 504
            CTICELAVVGADASGL+CSPTQLR
Sbjct: 1195 CTICELAVVGADASGLLCSPTQLR 1218



>AK110077 
          Length = 1242

 Score =  147 bits (371), Expect = 2e-35,   Method: Compositional matrix adjust.
 Identities = 108/303 (35%), Positives = 158/303 (52%), Gaps = 9/303 (2%)

Query: 203  WTQKSSLAGEHAAAGNFDTAMRLLSRQLGIKNFAPLKPLFLDLHMGSHSYLHALATAPII 262
            W + S++A +HAAAG+ +TAM LLSRQ GI +FAPLKPLFL   + + SYL A      I
Sbjct: 935  WLRNSAVAADHAAAGSVETAMTLLSRQAGIVDFAPLKPLFLSSFLAARSYLPAAPGVGPI 994

Query: 263  PVAVEKGWSESASPNVRGPPALVFTFPQMED-RLKAAYKATTDGKFPEALRQFLSILHTI 321
             V + +   ES     +  PA   +   +    L+  Y+A +  K  EA   F  +LH +
Sbjct: 995  EVHLRRNNEESDGKVTQAHPASPRSVKALASGELQEGYRAVSANKLAEAETIFRRLLHQL 1054

Query: 322  PLIVVDSRREVDEVKELIEIVREYVLGLRMELKRKELR----DDVNRQQELAAYFTNCKL 377
             L    S  E  E+++L+ + REY+LG+ +EL R++L     D+V R  ELAA FT+ +L
Sbjct: 1055 VLTPASSEAEATEIQDLVVLCREYILGVSIELGRRKLVQTEPDNVARNLELAALFTHTQL 1114

Query: 378  QRVHMRLVLGSAMGLCYKQKNFATAEHFARMLLENNPNEXXXXXXXXXXXXCS-GKKDSS 436
            Q  H  L L SAM    K  N A A  FA+ L+  +P                   +D+ 
Sbjct: 1115 QPQHQTLALRSAMTEARKVNNLAMAASFAKRLVALSPAPAVAQKAQQIISLAERSPRDAV 1174

Query: 437  EL-NYD-YRNPFVVC-GATYVPIYRGQKDVSCPYCGSRFVPSIEGQLCTICELAVVGADA 493
            E+  YD     FV+C G+  +    G   V+ P  G+R++P  +G LC + +++ VG  A
Sbjct: 1175 EVAGYDPLEQTFVICAGSHRLVTAAGAGSVADPLTGARYLPEFKGSLCKVSQISEVGRLA 1234

Query: 494  SGL 496
            SGL
Sbjct: 1235 SGL 1237



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.136    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 15,225,926
Number of extensions: 607746
Number of successful extensions: 1567
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 1561
Number of HSP's successfully gapped: 4
Length of query: 504
Length of database: 17,035,801
Length adjustment: 105
Effective length of query: 399
Effective length of database: 11,553,331
Effective search space: 4609779069
Effective search space used: 4609779069
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 158 (65.5 bits)