BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0666200 Os03g0666200|AK102364
         (500 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0666200  Pleckstrin homology-type domain containing pro...   873   0.0  
Os07g0138100  Pleckstrin homology-type domain containing pro...   530   e-151
Os05g0128700                                                      145   5e-35
Os05g0153600  FAR1 domain containing protein                       77   4e-14

>Os03g0666200 Pleckstrin homology-type domain containing protein
          Length = 500

 Score =  873 bits (2256), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 435/500 (87%), Positives = 435/500 (87%)

Query: 1   MATNGSSPRARETESSLEKVKRQLSSGSGRYLLQGPLLKRSETLRKWNERWVILDPTSGK 60
           MATNGSSPRARETESSLEKVKRQLSSGSGRYLLQGPLLKRSETLRKWNERWVILDPTSGK
Sbjct: 1   MATNGSSPRARETESSLEKVKRQLSSGSGRYLLQGPLLKRSETLRKWNERWVILDPTSGK 60

Query: 61  MEYKIRRNETAIKGTIIFDASSTITLSPVNFQGMPKYDGCCFYIGTPQKKDYFLCAETPG 120
           MEYKIRRNETAIKGTIIFDASSTITLSPVNFQGMPKYDGCCFYIGTPQKKDYFLCAETPG
Sbjct: 61  MEYKIRRNETAIKGTIIFDASSTITLSPVNFQGMPKYDGCCFYIGTPQKKDYFLCAETPG 120

Query: 121 AAKAWVSTLLATQLVLQAHKEAVNSLAGNGSPXXXXXXXXXXXXXXXXXXXXXKEIEAAM 180
           AAKAWVSTLLATQLVLQAHKEAVNSLAGNGSP                     KEIEAAM
Sbjct: 121 AAKAWVSTLLATQLVLQAHKEAVNSLAGNGSPATLGTVATAVANANATALEATKEIEAAM 180

Query: 181 KVSMRAALGLGANNPKEGQLDDLTIMKETLRVKDEELQNLAKDIRARDATIKEIANKLXX 240
           KVSMRAALGLGANNPKEGQLDDLTIMKETLRVKDEELQNLAKDIRARDATIKEIANKL  
Sbjct: 181 KVSMRAALGLGANNPKEGQLDDLTIMKETLRVKDEELQNLAKDIRARDATIKEIANKLTE 240

Query: 241 XXXXXXXXXXXXXXMDEQRRLLCSEIERLRQAMERQMEQSMLKLRQSEEKVISLSKEKDQ 300
                         MDEQRRLLCSEIERLRQAMERQMEQSMLKLRQSEEKVISLSKEKDQ
Sbjct: 241 TAEAAEAAASAAHTMDEQRRLLCSEIERLRQAMERQMEQSMLKLRQSEEKVISLSKEKDQ 300

Query: 301 LLKERDAALQEAHMWRTELGKAREQAVIQEATIARADXXXXXXXXXXXXXXXXXXXXLHA 360
           LLKERDAALQEAHMWRTELGKAREQAVIQEATIARAD                    LHA
Sbjct: 301 LLKERDAALQEAHMWRTELGKAREQAVIQEATIARADEKVRASEADAAARIKEAAEKLHA 360

Query: 361 VXXXXXXXXSLVGILQSQVQREQSSTKQVCEERSESCSGTDNSPPLTKHVDASDDDVDKA 420
           V        SLVGILQSQVQREQSSTKQVCEERSESCSGTDNSPPLTKHVDASDDDVDKA
Sbjct: 361 VEKEKEELLSLVGILQSQVQREQSSTKQVCEERSESCSGTDNSPPLTKHVDASDDDVDKA 420

Query: 421 CVSDSRSVLVSNDNTEVQLAVDGVDIRPIGDAEWGSFQQSEALIADVREVSPESEGGSLD 480
           CVSDSRSVLVSNDNTEVQLAVDGVDIRPIGDAEWGSFQQSEALIADVREVSPESEGGSLD
Sbjct: 421 CVSDSRSVLVSNDNTEVQLAVDGVDIRPIGDAEWGSFQQSEALIADVREVSPESEGGSLD 480

Query: 481 IPVVNPPPVSDHIQGGATHP 500
           IPVVNPPPVSDHIQGGATHP
Sbjct: 481 IPVVNPPPVSDHIQGGATHP 500



>Os07g0138100 Pleckstrin homology-type domain containing protein
          Length = 484

 Score =  530 bits (1366), Expect = e-151,   Method: Compositional matrix adjust.
 Identities = 279/496 (56%), Positives = 343/496 (69%), Gaps = 12/496 (2%)

Query: 1   MATNGSSPRARETESSLEKVKRQLSSGSGRYLLQGPLLKRSETLRKWNERWVILDPTSGK 60
           MA++GSS    E ESSLE+V+RQLSS S R+LLQGPLLKRS+TLRKWNERWVILDP +GK
Sbjct: 1   MASSGSSTVVGEMESSLERVRRQLSSTSSRHLLQGPLLKRSDTLRKWNERWVILDPATGK 60

Query: 61  MEYKIRRNETAIKGTIIFDASSTITLSPVNFQGMPKYDGCCFYIGTPQKKDYFLCAETPG 120
           MEYKIRR++ A++G I+FD++ST+TLSP+NF G+PKYDGCCFYIGTPQKK+YFLCAETP 
Sbjct: 61  MEYKIRRSDAAVRGIIVFDSTSTVTLSPMNFHGLPKYDGCCFYIGTPQKKEYFLCAETPS 120

Query: 121 AAKAWVSTLLATQLVLQAHKEAVNSLAGNGSPXXXXXXXXXXXXXXXXXXXXXKEIEAAM 180
           AA+AWVSTL A+QLVLQAHKEAVNSL GNG P                     KE+EAAM
Sbjct: 121 AARAWVSTLHASQLVLQAHKEAVNSLGGNG-PTKLGKVATVVAVANATAIEASKEVEAAM 179

Query: 181 KVSMRAALGLGANNPKEGQLDDLTIMKETLRVKDEELQNLAKDIRARDATIKEIANKLXX 240
           KVS+RAALG   N   +GQLDDLTIM ETLRVKD+EL  L +DIRARDATI+EI +KL  
Sbjct: 180 KVSLRAALGSTTNKLSKGQLDDLTIMMETLRVKDDELHQLLQDIRARDATIREITDKLQE 239

Query: 241 XXXXXXXXXXXXXXMDEQRRLLCSEIERLRQAMERQMEQSMLKLRQSEEKVISLSKEKDQ 300
                         +DEQRR L SE+ERL+Q  E+Q+E S+L+LR+SEEK   LS+E++ 
Sbjct: 240 TAEAAETAASAAHSIDEQRRFLSSELERLKQDQEKQIEFSLLRLRESEEKAKLLSEEREH 299

Query: 301 LLKERDAALQEAHMWRTELGKAREQAVIQEATIARADXXXXXXXXXXXXXXXXXXXXLHA 360
           LLKERD+ALQEA MWR+ELGKAR  AVI EA + RA+                    L +
Sbjct: 300 LLKERDSALQEAQMWRSELGKARGNAVILEAAVVRAEEKARVSAADADMRINDAASRLDS 359

Query: 361 VXXXXXXXXSLVGILQSQVQREQSSTKQVCEERSESCSGTDNSPPLTKHVDASDDDVDKA 420
                    +LV  LQ Q++ + +STKQVCEERSE CS +      +KHVD  DD+VDKA
Sbjct: 360 ATKEKEELVALVDALQLQIRSQDTSTKQVCEERSELCSTS------SKHVDMEDDNVDKA 413

Query: 421 CVSDSRSVLVSNDNTEVQLAVDGVDIRPIGDAEWGSFQQSEALIADVREVSPESEGGSLD 480
           C+SD+  + ++ +   V L  DGVDI  IG  EW +   SE  ++DVREV+ E E  SLD
Sbjct: 414 CLSDTDPIPITENI--VDLDDDGVDIPTIGVTEWNNPHSSE--VSDVREVTTEPEDNSLD 469

Query: 481 IPVVNPPPVSDHIQGG 496
           IP V+  PVS++   G
Sbjct: 470 IP-VDSQPVSENAFHG 484



>Os05g0128700 
          Length = 683

 Score =  145 bits (367), Expect = 5e-35,   Method: Compositional matrix adjust.
 Identities = 84/134 (62%), Positives = 92/134 (68%), Gaps = 16/134 (11%)

Query: 208 ETLRVKDEELQNLAKDIRARDATIKEIANKLXXXXXXXXXXXXXXXXMDEQRRLLCSEIE 267
           ETLRVKDEELQNLA +IRARDATIK+IA+KL                +D  R LLCSEIE
Sbjct: 534 ETLRVKDEELQNLANNIRARDATIKKIADKLTQTAEAAEAAASVAHTLDGHRILLCSEIE 593

Query: 268 RLRQAMERQMEQSMLKLRQSEEK----VISLSKEKDQLLKERDAALQEAHMWRTELGKAR 323
           RLR              +Q ++K       LSKEKDQLLKERDAALQEAHMWRTELGKAR
Sbjct: 594 RLRH------------YKQWKDKWNNPCSRLSKEKDQLLKERDAALQEAHMWRTELGKAR 641

Query: 324 EQAVIQEATIARAD 337
           EQAV+QEATIARAD
Sbjct: 642 EQAVVQEATIARAD 655



>Os05g0153600 FAR1 domain containing protein
          Length = 313

 Score = 76.6 bits (187), Expect = 4e-14,   Method: Compositional matrix adjust.
 Identities = 41/64 (64%), Positives = 44/64 (68%)

Query: 208 ETLRVKDEELQNLAKDIRARDATIKEIANKLXXXXXXXXXXXXXXXXMDEQRRLLCSEIE 267
           ETL+VKD ELQNLA +IRARDATIKEIA+KL                MDE RRLLCSEIE
Sbjct: 236 ETLQVKDGELQNLANNIRARDATIKEIADKLTQTAQAAEAAASATHTMDEHRRLLCSEIE 295

Query: 268 RLRQ 271
           RLR 
Sbjct: 296 RLRH 299



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.312    0.127    0.355 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 14,428,536
Number of extensions: 531634
Number of successful extensions: 1792
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 1780
Number of HSP's successfully gapped: 4
Length of query: 500
Length of database: 17,035,801
Length adjustment: 105
Effective length of query: 395
Effective length of database: 11,553,331
Effective search space: 4563565745
Effective search space used: 4563565745
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 158 (65.5 bits)