BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0645200 Os03g0645200|AK060478
         (150 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0645200  Similar to Translation initiation factor eIF-2...   312   6e-86
Os12g0617100  Similar to Translation initiation factor eIF-2...   273   3e-74
Os12g0497400  Initiation factor 2B related family protein          63   7e-11

>Os03g0645200 Similar to Translation initiation factor eIF-2B delta subunit
           (eIF-2B GDP-GTP exchange factor)
          Length = 150

 Score =  312 bits (799), Expect = 6e-86,   Method: Compositional matrix adjust.
 Identities = 150/150 (100%), Positives = 150/150 (100%)

Query: 1   KGINCTYTHINAISYIMHEVTRVFLGASSILSNGTVYSRVGTASVAMVAHAFGIPVLVCC 60
           KGINCTYTHINAISYIMHEVTRVFLGASSILSNGTVYSRVGTASVAMVAHAFGIPVLVCC
Sbjct: 1   KGINCTYTHINAISYIMHEVTRVFLGASSILSNGTVYSRVGTASVAMVAHAFGIPVLVCC 60

Query: 61  EAYKFHERVQLDSICANELGDPDVILKVPGKADLGHLKNLADNENLQLLNLTYDATPSDY 120
           EAYKFHERVQLDSICANELGDPDVILKVPGKADLGHLKNLADNENLQLLNLTYDATPSDY
Sbjct: 61  EAYKFHERVQLDSICANELGDPDVILKVPGKADLGHLKNLADNENLQLLNLTYDATPSDY 120

Query: 121 VSMIITDYGMLPPTSVPVIVREYRKEQLWI 150
           VSMIITDYGMLPPTSVPVIVREYRKEQLWI
Sbjct: 121 VSMIITDYGMLPPTSVPVIVREYRKEQLWI 150



>Os12g0617100 Similar to Translation initiation factor eIF-2B delta subunit
           (eIF-2B GDP-GTP exchange factor) (Guanine nucleotide
           exchange factor subunit GCD2) (GCD complex subunit GCD2)
          Length = 607

 Score =  273 bits (698), Expect = 3e-74,   Method: Compositional matrix adjust.
 Identities = 127/150 (84%), Positives = 136/150 (90%)

Query: 1   KGINCTYTHINAISYIMHEVTRVFLGASSILSNGTVYSRVGTASVAMVAHAFGIPVLVCC 60
           KGI+CTYTHINAISYIMHEVTRVFLGASSILSNG VYS VGTA+VAMVAHAFG+PVLVCC
Sbjct: 458 KGISCTYTHINAISYIMHEVTRVFLGASSILSNGAVYSSVGTAAVAMVAHAFGVPVLVCC 517

Query: 61  EAYKFHERVQLDSICANELGDPDVILKVPGKADLGHLKNLADNENLQLLNLTYDATPSDY 120
           EAYK HERVQLDSIC+NELGDPDVI +VP + DL HLKN   NENLQLLNL YD  PSDY
Sbjct: 518 EAYKLHERVQLDSICSNELGDPDVISRVPEREDLCHLKNCTYNENLQLLNLKYDTMPSDY 577

Query: 121 VSMIITDYGMLPPTSVPVIVREYRKEQLWI 150
           VSMIIT+YGMLPPTSVPVIVREYR+E +WI
Sbjct: 578 VSMIITEYGMLPPTSVPVIVREYRREHIWI 607



>Os12g0497400 Initiation factor 2B related family protein
          Length = 299

 Score = 63.2 bits (152), Expect = 7e-11,   Method: Compositional matrix adjust.
 Identities = 38/127 (29%), Positives = 64/127 (50%), Gaps = 15/127 (11%)

Query: 11  NAISYIMHEVTRVFLGASSILSNGTVYSRVGTASVAMVAHAFGIPVLVCCEAYKFHERVQ 70
           +A++Y M EV  VF+GA  ++ +G + + +GT  +A+VAH+   PV V  E+YKF     
Sbjct: 178 SAVAYSMDEVDMVFVGADGVVESGGIINMMGTYQIALVAHSMNKPVYVAAESYKFARLYP 237

Query: 71  LDSICANELGDP-DVILKVPGKADLGHLKNLADNENLQLLNLTYDATPSDYVSMIITDYG 129
           LD         P D  + +P                +++     D TP  Y+++++TD G
Sbjct: 238 LDQKDMTPAHRPIDFGVPIPA--------------GVEVETSARDYTPPQYLTLLLTDLG 283

Query: 130 MLPPTSV 136
           +L P+ V
Sbjct: 284 VLTPSVV 290



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.137    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,990,360
Number of extensions: 179026
Number of successful extensions: 407
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 407
Number of HSP's successfully gapped: 3
Length of query: 150
Length of database: 17,035,801
Length adjustment: 92
Effective length of query: 58
Effective length of database: 12,232,113
Effective search space: 709462554
Effective search space used: 709462554
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 151 (62.8 bits)