BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os03g0621700 Os03g0621700|AK109821
(137 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os03g0621700 Conserved hypothetical protein 244 2e-65
Os07g0164700 Similar to Helicase-like protein [Oryza sativa... 188 1e-48
Os10g0388500 Conserved hypothetical protein 67 3e-12
>Os03g0621700 Conserved hypothetical protein
Length = 137
Score = 244 bits (622), Expect = 2e-65, Method: Compositional matrix adjust.
Identities = 119/137 (86%), Positives = 119/137 (86%)
Query: 1 FDIHLYQPTDLGHEVVHPIVMPNSQPFADDRDASFSTPSNLPMCTGAHGDVTNLTHAEVV 60
FDIHLYQPTDLGHEVVHPIVMPNSQPFADDRDASFSTPSNLPMCTGAHGDVTNLTHAEVV
Sbjct: 1 FDIHLYQPTDLGHEVVHPIVMPNSQPFADDRDASFSTPSNLPMCTGAHGDVTNLTHAEVV 60
Query: 61 RKRARNWYASLTQEQXXXXXXXXXXXXXXXXXXSQVLNKSATNSGVAPLGKLSNISAADL 120
RKRARNWYASLTQEQ SQVLNKSATNSGVAPLGKLSNISAADL
Sbjct: 61 RKRARNWYASLTQEQKDERNKKDRERRKRKEEESQVLNKSATNSGVAPLGKLSNISAADL 120
Query: 121 MTCQLEVNDSSTLHKVY 137
MTCQLEVNDSSTLHKVY
Sbjct: 121 MTCQLEVNDSSTLHKVY 137
>Os07g0164700 Similar to Helicase-like protein [Oryza sativa (japonica
cultivar-group)]
Length = 210
Score = 188 bits (477), Expect = 1e-48, Method: Compositional matrix adjust.
Identities = 94/118 (79%), Positives = 95/118 (80%), Gaps = 1/118 (0%)
Query: 16 VHPIVMPNSQPFADDRDASFSTPSNLPMCTGAHGDVTNLTHAEVVRKRARNWYASLTQEQ 75
VHPIV PNSQ FADDRDASFSTPSNLPMCTGAHGDVTNLTHAEV RKRARNWY SLTQEQ
Sbjct: 71 VHPIVTPNSQSFADDRDASFSTPSNLPMCTGAHGDVTNLTHAEVARKRARNWYTSLTQEQ 130
Query: 76 XXXXXXXXXXXXXXXXXXSQVLNKSATNSGVAPLGKLSNISAADLMTCQLEVNDSSTL 133
SQVL KSATNSGVAPLGKLSN+SAADLMTCQLEVNDSSTL
Sbjct: 131 KDERNKKDHERRKRKKEESQVL-KSATNSGVAPLGKLSNVSAADLMTCQLEVNDSSTL 187
>Os10g0388500 Conserved hypothetical protein
Length = 531
Score = 67.4 bits (163), Expect = 3e-12, Method: Compositional matrix adjust.
Identities = 36/69 (52%), Positives = 43/69 (62%), Gaps = 3/69 (4%)
Query: 10 DLGHEVVHPIVMPNSQPFADDRDASFSTPSNLPM---CTGAHGDVTNLTHAEVVRKRARN 66
D G E V IV P PF + D S+STPS M AHGDVTNL+ +E+ KR+R
Sbjct: 392 DAGTENVGSIVTPVRLPFTNSSDMSYSTPSEYTMPLQAEDAHGDVTNLSASELKNKRSRE 451
Query: 67 WYASLTQEQ 75
WYASLT+EQ
Sbjct: 452 WYASLTKEQ 460
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.314 0.127 0.377
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,832,881
Number of extensions: 120644
Number of successful extensions: 287
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 285
Number of HSP's successfully gapped: 3
Length of query: 137
Length of database: 17,035,801
Length adjustment: 90
Effective length of query: 47
Effective length of database: 12,336,541
Effective search space: 579817427
Effective search space used: 579817427
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 150 (62.4 bits)