BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0374900 Os03g0374900|Os03g0374900
         (212 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0374900  Amino acid/polyamine transporter I family protein   381   e-106
Os03g0375300  Amino acid/polyamine transporter I family protein   182   2e-46
Os03g0375900  Amino acid/polyamine transporter I family protein   100   7e-22
Os02g0700500  Amino acid/polyamine transporter I family protein    72   4e-13

>Os03g0374900 Amino acid/polyamine transporter I family protein
          Length = 212

 Score =  381 bits (979), Expect = e-106,   Method: Compositional matrix adjust.
 Identities = 189/212 (89%), Positives = 189/212 (89%)

Query: 1   MAELGLLPSVFARRGPGRSATPWXXXXXXXXXXXXXXFLGFDDVVATANLLYSLGTLLEF 60
           MAELGLLPSVFARRGPGRSATPW              FLGFDDVVATANLLYSLGTLLEF
Sbjct: 1   MAELGLLPSVFARRGPGRSATPWVAVAASAAVSVAVSFLGFDDVVATANLLYSLGTLLEF 60

Query: 61  AAFLWLRCRGRHAAALKRPYRVXXXXXXXXXMCLVPSAFLAYVIAVAGWRVSAIAAGLTA 120
           AAFLWLRCRGRHAAALKRPYRV         MCLVPSAFLAYVIAVAGWRVSAIAAGLTA
Sbjct: 61  AAFLWLRCRGRHAAALKRPYRVPLPLPALAAMCLVPSAFLAYVIAVAGWRVSAIAAGLTA 120

Query: 121 LGVGWHGVMRVVSQCYMEHVFKYKVNHCQFKSMQGLSLITMYMYKRHVEERSVNTTAEKT 180
           LGVGWHGVMRVVSQCYMEHVFKYKVNHCQFKSMQGLSLITMYMYKRHVEERSVNTTAEKT
Sbjct: 121 LGVGWHGVMRVVSQCYMEHVFKYKVNHCQFKSMQGLSLITMYMYKRHVEERSVNTTAEKT 180

Query: 181 FGASWEAAIGVGFPNLHLEGGTIESPQRCQYL 212
           FGASWEAAIGVGFPNLHLEGGTIESPQRCQYL
Sbjct: 181 FGASWEAAIGVGFPNLHLEGGTIESPQRCQYL 212



>Os03g0375300 Amino acid/polyamine transporter I family protein
          Length = 163

 Score =  182 bits (462), Expect = 2e-46,   Method: Compositional matrix adjust.
 Identities = 95/131 (72%), Positives = 99/131 (75%)

Query: 1   MAELGLLPSVFARRGPGRSATPWXXXXXXXXXXXXXXFLGFDDVVATANLLYSLGTLLEF 60
           MAELGLLPSVFARRGPGRSATPW              FLGFDDVVATANLLYSLG LLEF
Sbjct: 1   MAELGLLPSVFARRGPGRSATPWVAVAASAAVSVAVSFLGFDDVVATANLLYSLGALLEF 60

Query: 61  AAFLWLRCRGRHAAALKRPYRVXXXXXXXXXMCLVPSAFLAYVIAVAGWRVSAIAAGLTA 120
           AAFL LR R    ++LKRPYRV         MCLVPSAFLAYV+AVAGWRVSA+AA LTA
Sbjct: 61  AAFLRLRAREESPSSLKRPYRVPLPLPALAAMCLVPSAFLAYVVAVAGWRVSAVAAALTA 120

Query: 121 LGVGWHGVMRV 131
           LGVGWHG MRV
Sbjct: 121 LGVGWHGAMRV 131



>Os03g0375900 Amino acid/polyamine transporter I family protein
          Length = 207

 Score =  100 bits (249), Expect = 7e-22,   Method: Compositional matrix adjust.
 Identities = 62/132 (46%), Positives = 76/132 (57%), Gaps = 5/132 (3%)

Query: 1   MAELGLLPSVFARRGPGRSATPWXXXXXXXXXXXXXXFLGFDDVVATANLLYSLGTLLEF 60
           MA+LGLLP  FA R P    TPW              F  FD +VA+AN LYSLG LLEF
Sbjct: 43  MADLGLLPRAFALRAPVFD-TPWVGILATAAITLAMSFTSFDTIVASANFLYSLGMLLEF 101

Query: 61  AAFLWLRCRGRHAAALKRPYRVXXX-XXXXXXMCLVPSAFLAYVIAVAGWRVSAIAAGLT 119
           AAF+ LR R     A+ RPY V          +C VPSAFL +V+A+AGW+V AI+A  T
Sbjct: 102 AAFVRLRAR---LPAMPRPYAVPLRGLPAAAALCAVPSAFLVFVMAIAGWKVYAISAVFT 158

Query: 120 ALGVGWHGVMRV 131
           A GV  + +M +
Sbjct: 159 AAGVAVYYLMDL 170



>Os02g0700500 Amino acid/polyamine transporter I family protein
          Length = 531

 Score = 71.6 bits (174), Expect = 4e-13,   Method: Compositional matrix adjust.
 Identities = 49/146 (33%), Positives = 71/146 (48%), Gaps = 8/146 (5%)

Query: 1   MAELGLLPSVFARRGPGRSATPWXXXXXXXXXXXXXXFLGFDDVVATANLLYSLGTLLEF 60
           MAE G+LPS FA R   R  TP                + F ++VA  N LY  G LLEF
Sbjct: 373 MAERGMLPSFFAARS--RYGTPLAGILFSASGVLLLSMMSFQEIVAAENFLYCFGMLLEF 430

Query: 61  AAFLWLRCRGRHAAALKRPYRVXXXXXXXXXMCLVPSAFLAYVIAVAGWRVSAIAAGLTA 120
            AF+  R R   AA   RPYRV         M + P+A +A V+A++  +V+ ++ G  A
Sbjct: 431 VAFILHRVRRPDAA---RPYRVPLGTAGCVAMLVPPTALIAVVLALSTLKVAVVSLGAVA 487

Query: 121 LGVGWHGVMRVVSQCYMEHVFKYKVN 146
           +G+     +R V +   +   ++ VN
Sbjct: 488 MGLVLQPALRFVEK---KRWLRFSVN 510



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.327    0.138    0.443 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,489,140
Number of extensions: 216984
Number of successful extensions: 565
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 562
Number of HSP's successfully gapped: 4
Length of query: 212
Length of database: 17,035,801
Length adjustment: 97
Effective length of query: 115
Effective length of database: 11,971,043
Effective search space: 1376669945
Effective search space used: 1376669945
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 154 (63.9 bits)