BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0355600 Os03g0355600|AK068938
         (893 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0355600  Similar to Adapter-related protein complex 2 b...  1674   0.0  
Os01g0973300  Adaptin, N-terminal domain containing protein       122   1e-27
Os01g0626100  Adaptin, N-terminal domain containing protein       122   1e-27

>Os03g0355600 Similar to Adapter-related protein complex 2 beta 1 subunit
           (Beta-adaptin) (Plasma membrane adaptor HA2/AP2 adaptin
           beta subunit) (Clathrin assembly protein complex 2 beta
           large chain) (AP105B) (Fragment)
          Length = 893

 Score = 1674 bits (4334), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 829/893 (92%), Positives = 829/893 (92%)

Query: 1   HDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAMTVGKDVSSLFTDVVNCMQTE 60
           HDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAMTVGKDVSSLFTDVVNCMQTE
Sbjct: 1   HDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAMTVGKDVSSLFTDVVNCMQTE 60

Query: 61  NLELKKLVYLYLINYAKSQPDLAILAVNTFVKDSQDPNPLIRALAVRTMGCIRVDKITEY 120
           NLELKKLVYLYLINYAKSQPDLAILAVNTFVKDSQDPNPLIRALAVRTMGCIRVDKITEY
Sbjct: 61  NLELKKLVYLYLINYAKSQPDLAILAVNTFVKDSQDPNPLIRALAVRTMGCIRVDKITEY 120

Query: 121 LCDPLQRCLKDDDPYVRKTAAICVAKLYDINAELVEDRGFLEALKDLISDNNPMXXXXXX 180
           LCDPLQRCLKDDDPYVRKTAAICVAKLYDINAELVEDRGFLEALKDLISDNNPM      
Sbjct: 121 LCDPLQRCLKDDDPYVRKTAAICVAKLYDINAELVEDRGFLEALKDLISDNNPMVVANAV 180

Query: 181 XXXXEIQDSSTRPIFEITSHTLSKLLTALNECTEWGQVFILDSLSRYKAADAREAENIVE 240
               EIQDSSTRPIFEITSHTLSKLLTALNECTEWGQVFILDSLSRYKAADAREAENIVE
Sbjct: 181 AALAEIQDSSTRPIFEITSHTLSKLLTALNECTEWGQVFILDSLSRYKAADAREAENIVE 240

Query: 241 RVTPRLQHANCAVVLSAVKIILLQMELITSTDVVRNLCKKMAPPLVTLLSAEPEIQYVAL 300
           RVTPRLQHANCAVVLSAVKIILLQMELITSTDVVRNLCKKMAPPLVTLLSAEPEIQYVAL
Sbjct: 241 RVTPRLQHANCAVVLSAVKIILLQMELITSTDVVRNLCKKMAPPLVTLLSAEPEIQYVAL 300

Query: 301 RNINLIVQKRPTILAHEIKVFFCKYNDPIYVKMEKLEIMIKLASDRNIDQVLLEFKEYAT 360
           RNINLIVQKRPTILAHEIKVFFCKYNDPIYVKMEKLEIMIKLASDRNIDQVLLEFKEYAT
Sbjct: 301 RNINLIVQKRPTILAHEIKVFFCKYNDPIYVKMEKLEIMIKLASDRNIDQVLLEFKEYAT 360

Query: 361 EVDVDFVRKAVRAIGRCAIKLERAAERCISVLLELIKIKVNYVVQEAIIVIKDIFRRYPN 420
           EVDVDFVRKAVRAIGRCAIKLERAAERCISVLLELIKIKVNYVVQEAIIVIKDIFRRYPN
Sbjct: 361 EVDVDFVRKAVRAIGRCAIKLERAAERCISVLLELIKIKVNYVVQEAIIVIKDIFRRYPN 420

Query: 421 TYESIIATLCESLDTLDEPEAKASMIWIIGEYAERIDNADELLESFLETFPEEPALVQLQ 480
           TYESIIATLCESLDTLDEPEAKASMIWIIGEYAERIDNADELLESFLETFPEEPALVQLQ
Sbjct: 421 TYESIIATLCESLDTLDEPEAKASMIWIIGEYAERIDNADELLESFLETFPEEPALVQLQ 480

Query: 481 LLTATVKLFLKKPTEGPQQMIQAVLNNATVETDNPDLRDRAYIYWRLLSTDPEAAKDVVL 540
           LLTATVKLFLKKPTEGPQQMIQAVLNNATVETDNPDLRDRAYIYWRLLSTDPEAAKDVVL
Sbjct: 481 LLTATVKLFLKKPTEGPQQMIQAVLNNATVETDNPDLRDRAYIYWRLLSTDPEAAKDVVL 540

Query: 541 AEKPVIXXXXXXXXXXXXXXXXANISTLSSVYHKPPEAFVSRVKTAPRADDEEFADTAET 600
           AEKPVI                ANISTLSSVYHKPPEAFVSRVKTAPRADDEEFADTAET
Sbjct: 541 AEKPVISDDSNQLDSSLLDDLLANISTLSSVYHKPPEAFVSRVKTAPRADDEEFADTAET 600

Query: 601 GYSESPSQGVDXXXXXXXXXXXXNVPVKQXXXXXXXXXXXDLLGDLMGMDNSIVPVDEPT 660
           GYSESPSQGVD            NVPVKQ           DLLGDLMGMDNSIVPVDEPT
Sbjct: 601 GYSESPSQGVDGASPSSSAGTSSNVPVKQPAAPAAPAPMPDLLGDLMGMDNSIVPVDEPT 660

Query: 661 AXXXXXXXXXXXXXXXQGLQISAQLVRRDGQIFYDISFDNGTQTVLDGFMIQFNKNTFGL 720
           A               QGLQISAQLVRRDGQIFYDISFDNGTQTVLDGFMIQFNKNTFGL
Sbjct: 661 APSGPPLPVLLPSTTGQGLQISAQLVRRDGQIFYDISFDNGTQTVLDGFMIQFNKNTFGL 720

Query: 721 AAGGALQVSPLQPGTSARTLLPMVAFQNLSPGAPSSLLQVAVKNNQQPVWYFNDKIPMHA 780
           AAGGALQVSPLQPGTSARTLLPMVAFQNLSPGAPSSLLQVAVKNNQQPVWYFNDKIPMHA
Sbjct: 721 AAGGALQVSPLQPGTSARTLLPMVAFQNLSPGAPSSLLQVAVKNNQQPVWYFNDKIPMHA 780

Query: 781 FFGEDGKMERTSFLEAWKSLPDDNEFSKEFPSSVVSSIDATVEHLAASNVFFIAKRKNSN 840
           FFGEDGKMERTSFLEAWKSLPDDNEFSKEFPSSVVSSIDATVEHLAASNVFFIAKRKNSN
Sbjct: 781 FFGEDGKMERTSFLEAWKSLPDDNEFSKEFPSSVVSSIDATVEHLAASNVFFIAKRKNSN 840

Query: 841 KDVLYMSAKIPRGIPFLIELTAAVGVPGVKCAVKTPNKEMVALFFEAMESLLK 893
           KDVLYMSAKIPRGIPFLIELTAAVGVPGVKCAVKTPNKEMVALFFEAMESLLK
Sbjct: 841 KDVLYMSAKIPRGIPFLIELTAAVGVPGVKCAVKTPNKEMVALFFEAMESLLK 893



>Os01g0973300 Adaptin, N-terminal domain containing protein
          Length = 927

 Score =  122 bits (307), Expect = 1e-27,   Method: Compositional matrix adjust.
 Identities = 98/310 (31%), Positives = 150/310 (48%), Gaps = 29/310 (9%)

Query: 238 IVERVTPRLQHANCAVVLSAVKIILLQMELITSTDVVRNLCKKMAPPLVTLLSAEPEIQY 297
           +++  +P L   N  V+L+A  +  +          V  L + + P L TL S+ P+  Y
Sbjct: 151 LLKCTSPLLWSRNSGVILAAASVHWIMAP-------VDQLNRVVGPILFTLRSS-PDATY 202

Query: 298 VALRNINLIVQKRPTILAHEIKVFFCKYNDPIYVKMEKLEIMIKLASDRNIDQVLLEFKE 357
           V L NI +  +  P + A   + FF   +DP   K  KLEI+  +A++ +I  +  EF++
Sbjct: 203 VMLGNILVFAKTAPQLFAPFYEDFFICTSDPYQTKALKLEILTTIATESSIPAIFEEFQD 262

Query: 358 YATEVDVDFVRKAVRAIGRCAIKLERAAERCISVLLEL------IKIKVNY-----VVQE 406
           Y  E D  FV   V AI  CA KL      C+  LL L      I    N+     V+ +
Sbjct: 263 YIKEPDRRFVADTVAAIALCAQKLPSITTSCLGGLLALVFYESSISDSANFDGEAAVLVQ 322

Query: 407 AIIVIKDIFRRYPNTYESIIATLCESLDTLDEPEAKASMIWIIGEYAERIDN-----ADE 461
           AI+ IK I R  P ++E +I  L  SLD + EP A++ +IWI GEY+  I N        
Sbjct: 323 AILSIKAIVRTDPASHEKVIVRLVHSLDKIKEPAARSLIIWIFGEYSS-IGNIIPKITPA 381

Query: 462 LLESFLETFPEEPALVQLQLLTATVKLFLKKPTEGPQQ---MIQAVLNNATVETDNPDLR 518
           +L+    +F  E    +LQ+L A  K+ +  P E  ++   ++  V+  AT +    D+R
Sbjct: 382 VLKYLAWSFAAEMLETKLQILNAAAKVIIHSPEEHLEEFKKIMAHVIKLATCDLSY-DVR 440

Query: 519 DRAYIYWRLL 528
           DRA    RLL
Sbjct: 441 DRARFISRLL 450



>Os01g0626100 Adaptin, N-terminal domain containing protein
          Length = 206

 Score =  122 bits (306), Expect = 1e-27,   Method: Composition-based stats.
 Identities = 68/165 (41%), Positives = 98/165 (59%), Gaps = 7/165 (4%)

Query: 11  KGEIPELKEELNS-------QYKDKRKDAVKKVIAAMTVGKDVSSLFTDVVNCMQTENLE 63
           K E+ +LK++L            D+R+D  K+VI+ MT G DVS+ F ++V C  T ++ 
Sbjct: 20  KSEVADLKQQLRQLAGSRAPDADDQRRDVFKRVISCMTAGIDVSAAFGEMVLCSATSDVV 79

Query: 64  LKKLVYLYLINYAKSQPDLAILAVNTFVKDSQDPNPLIRALAVRTMGCIRVDKITEYLCD 123
           LKK+ YLY+  +A++ PDLA+L +N   +D  D +P IR LA+R++  +RV  + EYL  
Sbjct: 80  LKKMCYLYVGVHARNHPDLALLTINFLQRDCHDQDPTIRGLALRSLCSLRVPNLVEYLVS 139

Query: 124 PLQRCLKDDDPYVRKTAAICVAKLYDINAELVEDRGFLEALKDLI 168
           PL   LKD   YVR  AA+  AKLY I+A    D     ALK L+
Sbjct: 140 PLATGLKDPSAYVRMIAAVGAAKLYHISATACLDADLPAALKALM 184



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.318    0.134    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 25,351,707
Number of extensions: 927145
Number of successful extensions: 2307
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 2303
Number of HSP's successfully gapped: 3
Length of query: 893
Length of database: 17,035,801
Length adjustment: 110
Effective length of query: 783
Effective length of database: 11,292,261
Effective search space: 8841840363
Effective search space used: 8841840363
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 161 (66.6 bits)