BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0326300 Os03g0326300|AK066058
         (445 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0326300  Zinc finger, RING-type domain containing protein    754   0.0  
Os10g0406200  Zinc finger, RING-type domain containing protein    151   8e-37
Os01g0261100  Zinc finger, RING-type domain containing protein     66   6e-11

>Os03g0326300 Zinc finger, RING-type domain containing protein
          Length = 445

 Score =  754 bits (1948), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 375/420 (89%), Positives = 375/420 (89%)

Query: 1   MDPNEPCWRMNSSFSPPMSRRWDCRNPSDGLPHRIHDAPPYVXXXXXXXXXXXXAFGSDQ 60
           MDPNEPCWRMNSSFSPPMSRRWDCRNPSDGLPHRIHDAPPYV            AFGSDQ
Sbjct: 1   MDPNEPCWRMNSSFSPPMSRRWDCRNPSDGLPHRIHDAPPYVSSLSSHSKGSRSAFGSDQ 60

Query: 61  YLNHQHSVSDGALSYFGSPADSLQAPRWTPSLQRFDLGEFSTPAGSRPDTSGYPQSSERQ 120
           YLNHQHSVSDGALSYFGSPADSLQAPRWTPSLQRFDLGEFSTPAGSRPDTSGYPQSSERQ
Sbjct: 61  YLNHQHSVSDGALSYFGSPADSLQAPRWTPSLQRFDLGEFSTPAGSRPDTSGYPQSSERQ 120

Query: 121 LTAVXXXXXXXXXXXXXXXXXXXKPPYLHLPRNHLGRRSFMSKPVYPLVFRNPVSEAEAS 180
           LTAV                   KPPYLHLPRNHLGRRSFMSKPVYPLVFRNPVSEAEAS
Sbjct: 121 LTAVSSFSSASPFSESSQLASSSKPPYLHLPRNHLGRRSFMSKPVYPLVFRNPVSEAEAS 180

Query: 181 GMPEASNIGRTTPSDDSPVWRRSLASPELKFHNALSEFRKVEASPEPNTSSRREGFRWSN 240
           GMPEASNIGRTTPSDDSPVWRRSLASPELKFHNALSEFRKVEASPEPNTSSRREGFRWSN
Sbjct: 181 GMPEASNIGRTTPSDDSPVWRRSLASPELKFHNALSEFRKVEASPEPNTSSRREGFRWSN 240

Query: 241 ASSYDFGYDGDAIDISDHISVESQRSPTSSVRFLKCGLCERFLRQKSPWTSNRIVQNSDM 300
           ASSYDFGYDGDAIDISDHISVESQRSPTSSVRFLKCGLCERFLRQKSPWTSNRIVQNSDM
Sbjct: 241 ASSYDFGYDGDAIDISDHISVESQRSPTSSVRFLKCGLCERFLRQKSPWTSNRIVQNSDM 300

Query: 301 PVAAVLPCRHVFHADCLEESTPKSQVHEPPCPLCTRGTDDEGCVSFSEPLHVALRSARRN 360
           PVAAVLPCRHVFHADCLEESTPKSQVHEPPCPLCTRGTDDEGCVSFSEPLHVALRSARRN
Sbjct: 301 PVAAVLPCRHVFHADCLEESTPKSQVHEPPCPLCTRGTDDEGCVSFSEPLHVALRSARRN 360

Query: 361 QXXXXXXXXXXXXXXANPSRSDHGLKRNQSALVARRSGTMFRNRFKKQFSFKGRFGKDLF 420
           Q              ANPSRSDHGLKRNQSALVARRSGTMFRNRFKKQFSFKGRFGKDLF
Sbjct: 361 QGNSFSLGGSGGSTSANPSRSDHGLKRNQSALVARRSGTMFRNRFKKQFSFKGRFGKDLF 420



>Os10g0406200 Zinc finger, RING-type domain containing protein
          Length = 498

 Score =  151 bits (382), Expect = 8e-37,   Method: Compositional matrix adjust.
 Identities = 121/351 (34%), Positives = 162/351 (46%), Gaps = 38/351 (10%)

Query: 4   NEPCWRMNSSFSPPMSRRWDCRNPSDGLPHRIHD----APPYVXXXXXXXXXXXXAFGSD 59
           ++P WR N+ FSPP+SRRW+ R  S+GL +        A  Y             ++   
Sbjct: 92  SDPLWRTNAGFSPPLSRRWEYRINSEGLSYGSQGDSGAAAHYGSSLSSNSKEPSRSWERS 151

Query: 60  QY--LNHQHSVSDGALSYFGSPADSLQAPR-WTPSLQRFDLGEFSTPAGSRPDTSGYPQS 116
                +H++S S+GA+SYF SP  + Q      P LQ   + E+   + + P   G    
Sbjct: 152 DVPPDHHRYSTSEGAISYFNSPDVTFQNHHIMLPMLQDSGIDEYMRVSVAEP--IGALLL 209

Query: 117 SERQLTAVXXXXXXXXXXXXXXXXXXXKPPYLHLPRNHLGRRSFMSKPVYPLVFRNPVSE 176
           SE  ++                        Y   PRN   RRSF+SKP++PL F      
Sbjct: 210 SE-GISGQQNSGGSTSSRSDGSEYDIVPKSYSSTPRNFPSRRSFLSKPIHPLSF------ 262

Query: 177 AEASGMPEASNIGRTTPSDDSPVWRRSLASPELKFHNALSEFRKVEASPEPNTS-SRREG 235
                 PE +  G+ T   DSPV   S +SP      A+ E R          S S  E 
Sbjct: 263 ------PEHALEGQET---DSPVANASTSSPMPSEFKAIGEIRPSGLMDYAYASGSHGES 313

Query: 236 FRWSNASSYDFGYDGDAIDISDHISVESQRSPTSSVRFL---KCGLCERFLRQKSPWTSN 292
             WS ASS D        D+S+    E +  P  S   +   +C LCER L ++SPW S 
Sbjct: 314 ANWSAASSMDL------TDLSERHDAE-RSGPLRSNNIMDRTRCDLCERLLSKRSPWGSR 366

Query: 293 RIVQNSDMPVAAVLPCRHVFHADCLEESTPKSQVHEPPCPLCTR--GTDDE 341
           RIV+  D+PVA VLPC HV+HA+CLE +TPK Q H+PPCP C R  G D E
Sbjct: 367 RIVRTGDLPVAGVLPCCHVYHAECLERTTPKGQKHDPPCPACDRLSGKDTE 417



>Os01g0261100 Zinc finger, RING-type domain containing protein
          Length = 431

 Score = 65.9 bits (159), Expect = 6e-11,   Method: Compositional matrix adjust.
 Identities = 26/62 (41%), Positives = 40/62 (64%), Gaps = 6/62 (9%)

Query: 276 CGLCERFLRQKSPWTSNRIVQNSDMPVAAVLPCRHVFHADCLEESTPKSQVHEPPCPLCT 335
           C LC + L+++S W +       ++ V AVL C HV+HADCL+  T ++  ++PPCP+CT
Sbjct: 284 CKLCLKLLKERSTWNAQ------ELAVVAVLLCGHVYHADCLDSLTAEADKYDPPCPVCT 337

Query: 336 RG 337
            G
Sbjct: 338 HG 339



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.317    0.132    0.415 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 15,241,795
Number of extensions: 626873
Number of successful extensions: 1686
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 1683
Number of HSP's successfully gapped: 3
Length of query: 445
Length of database: 17,035,801
Length adjustment: 104
Effective length of query: 341
Effective length of database: 11,605,545
Effective search space: 3957490845
Effective search space used: 3957490845
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 158 (65.5 bits)