BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0238800 Os03g0238800|AY224467
         (563 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0238800  Conserved hypothetical protein                      868   0.0  
Os10g0116900  Conserved hypothetical protein                      400   e-111
Os06g0138200  2OG-Fe(II) oxygenase domain containing protein      103   3e-22

>Os03g0238800 Conserved hypothetical protein
          Length = 563

 Score =  868 bits (2243), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 445/538 (82%), Positives = 445/538 (82%)

Query: 26  GWVVDERDGFISWLRGEFAAANAIIDXXXXXXRSVGEPGEFEHVAAAVQQRRHHWAPVIH 85
           GWVVDERDGFISWLRGEFAAANAIID      RSVGEPGEFEHVAAAVQQRRHHWAPVIH
Sbjct: 26  GWVVDERDGFISWLRGEFAAANAIIDLLLLHLRSVGEPGEFEHVAAAVQQRRHHWAPVIH 85

Query: 86  MQQFFPVGDVAYALQQAGWRRRAPPHHQQQGXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 145
           MQQFFPVGDVAYALQQAGWRRRAPPHHQQQG                             
Sbjct: 86  MQQFFPVGDVAYALQQAGWRRRAPPHHQQQGPGASPSPPPPPPRGRPSFSASHSHHRHGG 145

Query: 146 XXXXXXXXXXXXTGATAGSDKDGREVHNKEEKGMKEAENVVEAKSSQLESLVSHEGEKTP 205
                       TGATAGSDKDGREVHNKEEKGMKEAENVVEAKSSQLESLVSHEGEKTP
Sbjct: 146 HHHRSDSVRGGGTGATAGSDKDGREVHNKEEKGMKEAENVVEAKSSQLESLVSHEGEKTP 205

Query: 206 RPQAVAEGSSKVVPTPVEYTVNDIIDGKTVNAVEGLKVYEGLVNENEKNKILSLLNETKA 265
           RPQAVAEGSSKVVPTPVEYTVNDIIDGKTVNAVEGLKVYEGLVNENEKNKILSLLNETKA
Sbjct: 206 RPQAVAEGSSKVVPTPVEYTVNDIIDGKTVNAVEGLKVYEGLVNENEKNKILSLLNETKA 265

Query: 266 SFRRGGLEAGQTVIIGKRPMKGHGREIIQLGIPIVEGPPEDDYPRETKVEAVPGLLHDLF 325
           SFRRGGLEAGQTVIIGKRPMKGHGREIIQLGIPIVEGPPEDDYPRETKVEAVPGLLHDLF
Sbjct: 266 SFRRGGLEAGQTVIIGKRPMKGHGREIIQLGIPIVEGPPEDDYPRETKVEAVPGLLHDLF 325

Query: 326 DRLCQKEIIPTKPDYCVIDYYNEGDYSHPHQSPPWYGRPFCTFCLTDCDMVFGRVISGER 385
           DRLCQKEIIPTKPDYCVIDYYNEGDYSHPHQSPPWYGRPFCTFCLTDCDMVFGRVISGER
Sbjct: 326 DRLCQKEIIPTKPDYCVIDYYNEGDYSHPHQSPPWYGRPFCTFCLTDCDMVFGRVISGER 385

Query: 386 GDHRGPLKLSLSTGSLLVLHGKSADVAKRAIPAACKQRILLSFGKSLSRKQVPSESVSRF 445
           GDHRGPLKLSLSTGSLLVLHGKSADVAKRAIPAACKQRILLSFGKSLSRKQVPSESVSRF
Sbjct: 386 GDHRGPLKLSLSTGSLLVLHGKSADVAKRAIPAACKQRILLSFGKSLSRKQVPSESVSRF 445

Query: 446 XXXXXXXXXXXXXXXXANMARHSSSPKHFGYAPNSGVLXXXXXXXXXXXXSDGMQPLFXX 505
                           ANMARHSSSPKHFGYAPNSGVL            SDGMQPLF  
Sbjct: 446 TTPLTPPPMPWGPPRPANMARHSSSPKHFGYAPNSGVLPAPAIGAHHIPPSDGMQPLFVA 505

Query: 506 XXXXXXXXXXXXXXXXLPNSTTAWMAEAAPRSAPQRLPVPGTGVFLPPGSGHALPHQM 563
                           LPNSTTAWMAEAAPRSAPQRLPVPGTGVFLPPGSGHALPHQM
Sbjct: 506 PAPVAAAAMPFPSPVPLPNSTTAWMAEAAPRSAPQRLPVPGTGVFLPPGSGHALPHQM 563



>Os10g0116900 Conserved hypothetical protein
          Length = 595

 Score =  400 bits (1027), Expect = e-111,   Method: Compositional matrix adjust.
 Identities = 214/405 (52%), Positives = 264/405 (65%), Gaps = 10/405 (2%)

Query: 161 TAGSDKDGREVHNKEEKGMKEAENVVEA-KSSQLESLVSHEGEKTPRPQAVAEGSSKVVP 219
           +AG++ D   +      G+KE E   EA ++SQL S +SH  E   +PQ      S VVP
Sbjct: 119 SAGAEGDDAAI---ASGGVKEVETSAEATQNSQLVSHISHATEA--QPQKGLHVISNVVP 173

Query: 220 TPVEYTVNDIIDGKTVNAVEGLKVYEGLVNENEKNKILSLLNETKASFRRGGLEAGQTVI 279
            P  + VN++IDG+ VN +EGLK+Y+G V+  E  K+LS +NE K   R+ GLE GQTV+
Sbjct: 174 VPTCFVVNEVIDGRMVNVLEGLKLYKGYVDLTEIGKVLSFVNEAKTMRRKPGLE-GQTVV 232

Query: 280 IGKRPMKGHGREIIQLGIPIVEGPPEDDYPRETKVEAVPGLLHDLFDRLCQKEIIPTKPD 339
           + KRPMKGHGREIIQLG+PI EGPPED++ RE KV+ +PG+L +LFD L  ++++P+ PD
Sbjct: 233 VAKRPMKGHGREIIQLGLPITEGPPEDEHLREVKVDPIPGVLQNLFDSLVHQKVVPSSPD 292

Query: 340 YCVIDYYNEGDYSHPHQSPPWYGRPFCTFCLTDCDMVFGRVISGE-RGDHRGPLKLSLST 398
           YCVID +NEGDYSHPH  PPWYGRP CT CLTDCDMVFG VI+ + RGDH GPLKLSLST
Sbjct: 293 YCVIDIFNEGDYSHPHHHPPWYGRPICTLCLTDCDMVFGHVIAADSRGDHAGPLKLSLST 352

Query: 399 GSLLVLHGKSADVAKRAIPAACKQRILLSFGKSLSRKQVPSESVSRFXXXXXXXXXX-XX 457
           GS+LV  GKSAD+AKRA+PA  KQRILLSFGKS+SRK V SES                 
Sbjct: 353 GSVLVFEGKSADIAKRALPATSKQRILLSFGKSVSRKHVQSESSLLITPPLTPPPMPWAA 412

Query: 458 XXXXANMARHSSSPKHFGYAPNSGVLXXXXXXXXXXXXSDGMQPLFXXXXXXXXXXXXXX 517
                N+A H SSPK   Y P++ V             S+G+Q +F              
Sbjct: 413 PLRPGNIAIHPSSPKQLVYNPSNRV-PAVSTPGLHHIPSNGIQTVFVAPLPITPKAVPFA 471

Query: 518 XXXXLPNSTTAWMAEAAPRSAPQRLPVPGTGVFLPPGSGHALPHQ 562
               LPNST AW+AEAAPR A  RLP+ GTGVFLPPGSG+  P Q
Sbjct: 472 SAVTLPNSTAAWIAEAAPRPASPRLPLQGTGVFLPPGSGNPPPAQ 516




 Score = 95.5 bits (236), Expect = 8e-20,   Method: Compositional matrix adjust.
 Identities = 41/66 (62%), Positives = 50/66 (75%)

Query: 30 DERDGFISWLRGEFAAANAIIDXXXXXXRSVGEPGEFEHVAAAVQQRRHHWAPVIHMQQF 89
          D RDG ++WLRGEFAAANAIID      R   +P  F+ VAAAVQ+RRHHWAPV+H+Q +
Sbjct: 28 DGRDGLVAWLRGEFAAANAIIDLLLAHARDAADPAGFDAVAAAVQRRRHHWAPVLHLQHY 87

Query: 90 FPVGDV 95
          FPV +V
Sbjct: 88 FPVTEV 93



>Os06g0138200 2OG-Fe(II) oxygenase domain containing protein
          Length = 616

 Score =  103 bits (257), Expect = 3e-22,   Method: Compositional matrix adjust.
 Identities = 65/217 (29%), Positives = 115/217 (52%), Gaps = 11/217 (5%)

Query: 223 EYTVNDIIDGKTVNAVEGLKVYEGLVNENEKNKILSLLNETKASFRRGGLEAGQTVIIGK 282
           ++   + ++G+ VN + GL+++ G+ +  E+ +I+  + + +   + G L   +T    +
Sbjct: 187 DFICFERVNGRLVNILAGLELHCGVFSAAEQKRIVDYVYDLQEMGKHGEL-GDRTYTEPQ 245

Query: 283 RPMKGHGREIIQLGIPIVEGPPEDDYP----RETKVEAVPGLLHDLFDRLCQKEIIPTK- 337
           R M+G GR  IQ G        ++  P    R    + +P L   +  RL +  ++P   
Sbjct: 246 RWMRGKGRVTIQFGCCYNYATDKNGNPPGIIRTIASDPMPSLFKIMIKRLVRWHVLPKTC 305

Query: 338 -PDYCVIDYYNEGDYSHPHQSPPWYGRPFCTFC-LTDCDMVFGRV--ISGERGDHRGPLK 393
            PD C+++ Y+ GD   PH     + RPFCT   L++C+++FG    I+G  G+  G L 
Sbjct: 306 IPDSCIVNIYDPGDCIPPHIDSHDFVRPFCTVSFLSECNILFGSTLKIAGP-GEFTGSLP 364

Query: 394 LSLSTGSLLVLHGKSADVAKRAIPAACKQRILLSFGK 430
           + L  GS+L+L+G  ADVAK  +PA   +RI ++F K
Sbjct: 365 IPLPVGSVLILNGNGADVAKHCVPAVPTKRISITFRK 401



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.316    0.135    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 17,649,417
Number of extensions: 724276
Number of successful extensions: 2079
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 2072
Number of HSP's successfully gapped: 5
Length of query: 563
Length of database: 17,035,801
Length adjustment: 106
Effective length of query: 457
Effective length of database: 11,501,117
Effective search space: 5256010469
Effective search space used: 5256010469
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 159 (65.9 bits)