BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0222600 Os03g0222600|J075095E18
         (125 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0222600  Caleosin related family protein                     226   2e-60
Os04g0511200  EFA27 for EF hand, abscisic acid, 27kD              107   3e-24
Os04g0510900  Similar to Embryo-specific protein 1 (ATS1)          91   3e-19

>Os03g0222600 Caleosin related family protein
          Length = 125

 Score =  226 bits (577), Expect = 2e-60,   Method: Compositional matrix adjust.
 Identities = 112/125 (89%), Positives = 112/125 (89%)

Query: 1   MATLLLIACLPTRRPHLSLRQRSYKLAPRITMAXXXXXXXXXXXXXLETVAPHAAVTGER 60
           MATLLLIACLPTRRPHLSLRQRSYKLAPRITMA             LETVAPHAAVTGER
Sbjct: 1   MATLLLIACLPTRRPHLSLRQRSYKLAPRITMASSSSSSPPSSDPSLETVAPHAAVTGER 60

Query: 61  KLNPNLQEQLPKPYLARALAAVDPSHPQGTRGRDARGMSVLQQHAAFFDRNGDGIIYPWE 120
           KLNPNLQEQLPKPYLARALAAVDPSHPQGTRGRDARGMSVLQQHAAFFDRNGDGIIYPWE
Sbjct: 61  KLNPNLQEQLPKPYLARALAAVDPSHPQGTRGRDARGMSVLQQHAAFFDRNGDGIIYPWE 120

Query: 121 TFQGE 125
           TFQGE
Sbjct: 121 TFQGE 125



>Os04g0511200 EFA27 for EF hand, abscisic acid, 27kD
          Length = 244

 Score =  107 bits (266), Expect = 3e-24,   Method: Compositional matrix adjust.
 Identities = 50/78 (64%), Positives = 59/78 (75%)

Query: 47  LETVAPHAAVTGERKLNPNLQEQLPKPYLARALAAVDPSHPQGTRGRDARGMSVLQQHAA 106
           L +VA  A VT ER +  +L+ Q+PKPYLARAL A D  HP+GT GRD R MSVLQQH A
Sbjct: 15  LSSVAAEAPVTRERPVRADLEVQIPKPYLARALVAPDVYHPEGTEGRDHRQMSVLQQHVA 74

Query: 107 FFDRNGDGIIYPWETFQG 124
           FFD +GDGI+YPWET+ G
Sbjct: 75  FFDLDGDGIVYPWETYGG 92



>Os04g0510900 Similar to Embryo-specific protein 1 (ATS1)
          Length = 310

 Score = 90.5 bits (223), Expect = 3e-19,   Method: Compositional matrix adjust.
 Identities = 46/80 (57%), Positives = 56/80 (70%), Gaps = 2/80 (2%)

Query: 47  LETVAPHAAVTGERKLNPNLQEQLPKPYLARALAAVDPSHPQGTRGRDARG--MSVLQQH 104
           L  VA  + VT ER +  +L+E +PKPYLARALAA D  HP GT   D     +SVLQQH
Sbjct: 80  LTIVALQSPVTVERPVRGDLEEHVPKPYLARALAAPDIYHPDGTTTDDHEHHHLSVLQQH 139

Query: 105 AAFFDRNGDGIIYPWETFQG 124
            AFFDR+ +GIIYPWET++G
Sbjct: 140 VAFFDRDDNGIIYPWETYEG 159



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.135    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,066,145
Number of extensions: 144239
Number of successful extensions: 333
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 332
Number of HSP's successfully gapped: 3
Length of query: 125
Length of database: 17,035,801
Length adjustment: 88
Effective length of query: 37
Effective length of database: 12,440,969
Effective search space: 460315853
Effective search space used: 460315853
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 149 (62.0 bits)