BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0216500 Os03g0216500|Os03g0216500
         (350 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0216500  Conserved hypothetical protein                      595   e-170
Os01g0228500  Harpin-induced 1 domain containing protein           68   1e-11
Os06g0163300  Harpin-induced 1 domain containing protein           67   3e-11

>Os03g0216500 Conserved hypothetical protein
          Length = 350

 Score =  595 bits (1535), Expect = e-170,   Method: Compositional matrix adjust.
 Identities = 298/350 (85%), Positives = 298/350 (85%)

Query: 1   MAGGTGIGADEQELPLFHPSPCAYYVQSPSAASHTLSHPASESTAIILSPFPDAAFAAPR 60
           MAGGTGIGADEQELPLFHPSPCAYYVQSPSAASHTLSHPASESTAIILSPFPDAAFAAPR
Sbjct: 1   MAGGTGIGADEQELPLFHPSPCAYYVQSPSAASHTLSHPASESTAIILSPFPDAAFAAPR 60

Query: 61  HSRVVDDATAHDNDVDQEAXXXXXXXXXXXXXXXXXXXAADKKLPAGGGHRGRQVLXXXX 120
           HSRVVDDATAHDNDVDQEA                   AADKKLPAGGGHRGRQVL    
Sbjct: 61  HSRVVDDATAHDNDVDQEASRLTLSRYSSSRGSNNSFLAADKKLPAGGGHRGRQVLSGRS 120

Query: 121 XXXXXXXXXXXXXXARSGAWRYVKLDPDAPCCCIVFQVAWRXXXXXXXXXXXXXXXTKPR 180
                         ARSGAWRYVKLDPDAPCCCIVFQVAWR               TKPR
Sbjct: 121 GGGVDEEEEDDSEGARSGAWRYVKLDPDAPCCCIVFQVAWRVAVSVAFALLVFFVATKPR 180

Query: 181 DPGVTFKVGKIQQFSLGEGLDGSGVITSFLSCNLTVAMAVDNHSRVFTLHVRPPRLDMSF 240
           DPGVTFKVGKIQQFSLGEGLDGSGVITSFLSCNLTVAMAVDNHSRVFTLHVRPPRLDMSF
Sbjct: 181 DPGVTFKVGKIQQFSLGEGLDGSGVITSFLSCNLTVAMAVDNHSRVFTLHVRPPRLDMSF 240

Query: 241 GRFTFATSQGVEEEGSSYDVGARGKRTVRLFVAAEGKPMYAAGRGMQDLLESGGGLPLAV 300
           GRFTFATSQGVEEEGSSYDVGARGKRTVRLFVAAEGKPMYAAGRGMQDLLESGGGLPLAV
Sbjct: 241 GRFTFATSQGVEEEGSSYDVGARGKRTVRLFVAAEGKPMYAAGRGMQDLLESGGGLPLAV 300

Query: 301 TVRARSRYRMVGSLVRLSYRHDAQCVVRLRRTPRRNNAIDASGYTCSAIR 350
           TVRARSRYRMVGSLVRLSYRHDAQCVVRLRRTPRRNNAIDASGYTCSAIR
Sbjct: 301 TVRARSRYRMVGSLVRLSYRHDAQCVVRLRRTPRRNNAIDASGYTCSAIR 350



>Os01g0228500 Harpin-induced 1 domain containing protein
          Length = 330

 Score = 67.8 bits (164), Expect = 1e-11,   Method: Compositional matrix adjust.
 Identities = 44/150 (29%), Positives = 73/150 (48%), Gaps = 10/150 (6%)

Query: 182 PGVTFKVGKIQQFSLGEGLDGSGVITSFLSCNLTVAMAVDNHSRVFTLHVRPPRLDMSFG 241
           PGV  K   +  F  GEG+D +GV T  ++ N ++ + V N S +F +HV    + + F 
Sbjct: 171 PGVLVKSLTVGNFYAGEGIDRTGVPTKLVTMNCSLQINVHNPSTMFGIHVSSTSIQILFS 230

Query: 242 RFTFATSQ--GVEEEGSSYDVGARGKRTVRLFVAAEGKPMYAAGRGMQDLLESGGGLPLA 299
           +   A  Q     +  SS+ V +         V  E  P+Y AG     L  +GG +PL 
Sbjct: 231 QIAIANGQLEKFYQPRSSHHVASA-------IVHGEKIPLYGAGETFA-LSNAGGAVPLT 282

Query: 300 VTVRARSRYRMVGSLVRLSYRHDAQCVVRL 329
           + +  R+R  ++G LVR+++    +C V +
Sbjct: 283 LDLVVRARGYVIGKLVRVTHTKRVKCPVVI 312



>Os06g0163300 Harpin-induced 1 domain containing protein
          Length = 322

 Score = 66.6 bits (161), Expect = 3e-11,   Method: Compositional matrix adjust.
 Identities = 46/157 (29%), Positives = 74/157 (47%), Gaps = 8/157 (5%)

Query: 178 KPRDPGVTFKVGKIQQFSLGEGLDGSGVITSFLSCNLTVAMAVDNHSRVFTLHVRPPRLD 237
           +P +P V  K   +  F  GEG D SGV T  ++ N ++ +AV N + +F +HV    + 
Sbjct: 157 RPYEPDVVVKSLTMDDFYAGEGTDHSGVPTKLVTLNCSLHIAVYNPASMFGIHVTTGPIR 216

Query: 238 MSFGRFTFATSQGVEEEGSSYDVGARGKRTVRLFVAAEGKPMYAAGRGMQDLLESGGGLP 297
           + +   +    Q        Y    +  R V   V     P+Y AG G+  L  SGG +P
Sbjct: 217 LLYSEISIGVGQ-----VRRYYQPRKSHRLVTAVVHGNKVPLYGAGGGLM-LSSSGGAVP 270

Query: 298 LAVTVRARSRYRMVGSLVRLSYRHDAQC--VVRLRRT 332
           L +     SR  ++G LVR++++    C  VV  ++T
Sbjct: 271 LTLDFDLTSRGYVIGKLVRVTHKVHVTCPIVVDAKKT 307



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.135    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 11,744,010
Number of extensions: 460659
Number of successful extensions: 1155
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 1154
Number of HSP's successfully gapped: 3
Length of query: 350
Length of database: 17,035,801
Length adjustment: 102
Effective length of query: 248
Effective length of database: 11,709,973
Effective search space: 2904073304
Effective search space used: 2904073304
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 156 (64.7 bits)