BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0212400 Os03g0212400|AK064327
         (251 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0212400  Similar to SNAP-28                                  484   e-137
Os02g0437200  Similar to SNAP-34                                  229   1e-60
Os02g0529500  Similar to SNAP25-like protein C (Fragment)         220   8e-58

>Os03g0212400 Similar to SNAP-28
          Length = 251

 Score =  484 bits (1245), Expect = e-137,   Method: Compositional matrix adjust.
 Identities = 237/251 (94%), Positives = 237/251 (94%)

Query: 1   IKTVPILMDKKQTSLGTPVYRTNPFDSDSDSEVPSRPSRAQSVPVRRTDQSIQELEDYAV 60
           IKTVPILMDKKQTSLGTPVYRTNPFDSDSDSEVPSRPSRAQSVPVRRTDQSIQELEDYAV
Sbjct: 1   IKTVPILMDKKQTSLGTPVYRTNPFDSDSDSEVPSRPSRAQSVPVRRTDQSIQELEDYAV 60

Query: 61  DKVEETSRKVNDCVRAAEAIREDATKTLVTLHRQGEQITRTHRVAADIEHDXXXXXXXXX 120
           DKVEETSRKVNDCVRAAEAIREDATKTLVTLHRQGEQITRTHRVAADIEHD         
Sbjct: 61  DKVEETSRKVNDCVRAAEAIREDATKTLVTLHRQGEQITRTHRVAADIEHDLSMSEKLLG 120

Query: 121 XXXXXFSKTWKPKRNQQIKGPISQNNSFTSSANHMEQRQRLGISSTRQPSPNQVHRSPAT 180
                FSKTWKPKRNQQIKGPISQNNSFTSSANHMEQRQRLGISSTRQPSPNQVHRSPAT
Sbjct: 121 SLGGLFSKTWKPKRNQQIKGPISQNNSFTSSANHMEQRQRLGISSTRQPSPNQVHRSPAT 180

Query: 181 AIEKVQVEIAKQDDALSDLSNMLGELKGMALDMGTEIERQNKSLDAFGDDVDELNFRVKG 240
           AIEKVQVEIAKQDDALSDLSNMLGELKGMALDMGTEIERQNKSLDAFGDDVDELNFRVKG
Sbjct: 181 AIEKVQVEIAKQDDALSDLSNMLGELKGMALDMGTEIERQNKSLDAFGDDVDELNFRVKG 240

Query: 241 ANQRGRRLLGK 251
           ANQRGRRLLGK
Sbjct: 241 ANQRGRRLLGK 251



>Os02g0437200 Similar to SNAP-34
          Length = 283

 Score =  229 bits (584), Expect = 1e-60,   Method: Compositional matrix adjust.
 Identities = 128/271 (47%), Positives = 166/271 (61%), Gaps = 37/271 (13%)

Query: 18  PVYRTNPFDSDSD-SEVPSRPSRAQSVP-------------------------VRRTD-- 49
           P   +NPFDSDSD      RP+RA SVP                           R D  
Sbjct: 13  PSRSSNPFDSDSDDGGREQRPARASSVPPPADQRGSLFGGGDGFSASSAAARSRYRNDFR 72

Query: 50  -------QSIQELEDYAVDKVEETSRKVNDCVRAAEAIREDATKTLVTLHRQGEQITRTH 102
                  QS+QELE YA  K EET+++V  CVR AE +R+ A+K+LVT+H+QG+QITRTH
Sbjct: 73  DTGGVEAQSVQELEGYAAYKAEETTQRVQGCVRIAEEMRDTASKSLVTIHQQGQQITRTH 132

Query: 103 RVAADIEHDXXXXXXXXXXXXXXFSKTWKPKRNQQIKGP-ISQNNSFTSSANHMEQRQRL 161
            +  DI+ D              FSK WKPK+N +I+GP +++++SF    +H+EQR +L
Sbjct: 133 MMTLDIDQDLSRSEKLLGDLGGIFSKKWKPKKNGEIRGPMLTRDDSFIRKGSHLEQRHKL 192

Query: 162 GISS-TRQPSPNQVHRSPATAIEKVQVEIAKQDDALSDLSNMLGELKGMALDMGTEIERQ 220
           G+S    Q +  Q H  P +A++KV++E AKQDD LSDLSN+L ELKGMA+DMGTEI+RQ
Sbjct: 193 GLSDHPPQSNARQFHSEPTSALQKVEMEKAKQDDGLSDLSNILTELKGMAVDMGTEIDRQ 252

Query: 221 NKSLDAFGDDVDELNFRVKGANQRGRRLLGK 251
            K+L     D DELNFR+KGAN R RRLLGK
Sbjct: 253 TKALGDSEKDYDELNFRIKGANTRARRLLGK 283



>Os02g0529500 Similar to SNAP25-like protein C (Fragment)
          Length = 259

 Score =  220 bits (560), Expect = 8e-58,   Method: Compositional matrix adjust.
 Identities = 112/203 (55%), Positives = 145/203 (71%), Gaps = 2/203 (0%)

Query: 50  QSIQELEDYAVDKVEETSRKVNDCVRAAEAIREDATKTLVTLHRQGEQITRTHRVAADIE 109
           QS+QELE+YA  K EET+  +  C+R AE IREDA  TLVTL++QG+QI+RTH  A +I+
Sbjct: 58  QSVQELENYAAYKAEETTDALGGCLRIAENIREDAANTLVTLNKQGQQISRTHEKAVEID 117

Query: 110 HDXXXXXXXXXXXXXXFSKTWKPKRNQQIKGPIS-QNNSFTSSANHMEQRQRLGISSTRQ 168
            D              FSK WKPK+ +QIKGP    ++SF   A+H+EQR++LG+S + +
Sbjct: 118 QDLSKGESLLGSLGGFFSKPWKPKKTRQIKGPAHVSDDSFKKKASHIEQREKLGLSPSGK 177

Query: 169 PSPNQVHRSPATAIEKVQVEIAKQDDALSDLSNMLGELKGMALDMGTEIERQNKSLDAFG 228
            S N+ +  P TA+EKVQVE  KQDDAL DLS +LG+LKGMA DMG+E++RQNK+LD   
Sbjct: 178 -SANRSYAEPTTAMEKVQVEKQKQDDALDDLSGVLGQLKGMACDMGSELDRQNKALDDLQ 236

Query: 229 DDVDELNFRVKGANQRGRRLLGK 251
            DVDELN RVKGANQR R+L+ K
Sbjct: 237 GDVDELNSRVKGANQRARKLIEK 259



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.311    0.127    0.347 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 7,435,203
Number of extensions: 282575
Number of successful extensions: 1034
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 1029
Number of HSP's successfully gapped: 3
Length of query: 251
Length of database: 17,035,801
Length adjustment: 98
Effective length of query: 153
Effective length of database: 11,918,829
Effective search space: 1823580837
Effective search space used: 1823580837
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 155 (64.3 bits)