BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os02g0827100 Os02g0827100|AK058935
(40 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os02g0827100 Hypothetical protein 87 3e-18
Os10g0497800 Similar to Prolyl 4-hydroxylase, alpha subunit... 75 1e-14
Os03g0166200 Similar to Prolyl 4-hydroxylase alpha-1 subuni... 64 2e-11
>Os02g0827100 Hypothetical protein
Length = 40
Score = 87.0 bits (214), Expect = 3e-18, Method: Compositional matrix adjust.
Identities = 40/40 (100%), Positives = 40/40 (100%)
Query: 1 FWSMKPDATLDPLSLHGGCPVIKGNKWSSTKWMHVREYKA 40
FWSMKPDATLDPLSLHGGCPVIKGNKWSSTKWMHVREYKA
Sbjct: 1 FWSMKPDATLDPLSLHGGCPVIKGNKWSSTKWMHVREYKA 40
>Os10g0497800 Similar to Prolyl 4-hydroxylase, alpha subunit-like protein
Length = 321
Score = 75.1 bits (183), Expect = 1e-14, Method: Compositional matrix adjust.
Identities = 32/39 (82%), Positives = 34/39 (87%)
Query: 1 FWSMKPDATLDPLSLHGGCPVIKGNKWSSTKWMHVREYK 39
FWSM+PD +LD SLHGGCPVIKGNKWSSTKWM V EYK
Sbjct: 282 FWSMRPDGSLDATSLHGGCPVIKGNKWSSTKWMRVHEYK 320
>Os03g0166200 Similar to Prolyl 4-hydroxylase alpha-1 subunit-like protein
Length = 135
Score = 64.3 bits (155), Expect = 2e-11, Method: Compositional matrix adjust.
Identities = 26/38 (68%), Positives = 32/38 (84%)
Query: 1 FWSMKPDATLDPLSLHGGCPVIKGNKWSSTKWMHVREY 38
F+S+ P+AT DP SLHG CPVI+G KWS+TKW+HVR Y
Sbjct: 45 FFSLYPNATFDPGSLHGSCPVIQGEKWSATKWIHVRSY 82
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.319 0.133 0.480
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 1,877,931
Number of extensions: 45302
Number of successful extensions: 93
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 93
Number of HSP's successfully gapped: 5
Length of query: 40
Length of database: 17,035,801
Length adjustment: 14
Effective length of query: 26
Effective length of database: 16,304,805
Effective search space: 423924930
Effective search space used: 423924930
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 149 (62.0 bits)