BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os02g0503400 Os02g0503400|AK119704
(123 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os02g0503400 Similar to 60S ribosomal protein L35 236 2e-63
Os04g0376000 Similar to 60S ribosomal protein L35-1 232 5e-62
Os06g0732000 Similar to Ribosomal protein L35 129 6e-31
>Os02g0503400 Similar to 60S ribosomal protein L35
Length = 123
Score = 236 bits (603), Expect = 2e-63, Method: Compositional matrix adjust.
Identities = 123/123 (100%), Positives = 123/123 (100%)
Query: 1 MARIKVDELRGKNKAELQAQLKDLKAELSLLRVAKVTGGAPNKLSKIKVVRTSIARVLTV 60
MARIKVDELRGKNKAELQAQLKDLKAELSLLRVAKVTGGAPNKLSKIKVVRTSIARVLTV
Sbjct: 1 MARIKVDELRGKNKAELQAQLKDLKAELSLLRVAKVTGGAPNKLSKIKVVRTSIARVLTV 60
Query: 61 ISQKQRAALREAYKKKSLLPLDLRPKKTRAIRRRLTKHQLSLKTEREKKREKYFPMRKYA 120
ISQKQRAALREAYKKKSLLPLDLRPKKTRAIRRRLTKHQLSLKTEREKKREKYFPMRKYA
Sbjct: 61 ISQKQRAALREAYKKKSLLPLDLRPKKTRAIRRRLTKHQLSLKTEREKKREKYFPMRKYA 120
Query: 121 IKA 123
IKA
Sbjct: 121 IKA 123
>Os04g0376000 Similar to 60S ribosomal protein L35-1
Length = 123
Score = 232 bits (591), Expect = 5e-62, Method: Compositional matrix adjust.
Identities = 120/123 (97%), Positives = 122/123 (99%)
Query: 1 MARIKVDELRGKNKAELQAQLKDLKAELSLLRVAKVTGGAPNKLSKIKVVRTSIARVLTV 60
MARIKV ELRGKNKAELQAQLKDLKAELSLLRVAKVTGGAPNKLSKIKVVRTSIARVLTV
Sbjct: 1 MARIKVHELRGKNKAELQAQLKDLKAELSLLRVAKVTGGAPNKLSKIKVVRTSIARVLTV 60
Query: 61 ISQKQRAALREAYKKKSLLPLDLRPKKTRAIRRRLTKHQLSLKTEREKKREKYFPMRKYA 120
ISQKQ+AALREAYKKKSLLPLDLRPKKTRAIRRRLTKHQLSLKTEREKKREKYFPMRKYA
Sbjct: 61 ISQKQKAALREAYKKKSLLPLDLRPKKTRAIRRRLTKHQLSLKTEREKKREKYFPMRKYA 120
Query: 121 IKA 123
+KA
Sbjct: 121 VKA 123
>Os06g0732000 Similar to Ribosomal protein L35
Length = 183
Score = 129 bits (323), Expect = 6e-31, Method: Compositional matrix adjust.
Identities = 76/101 (75%), Positives = 79/101 (78%), Gaps = 18/101 (17%)
Query: 1 MARIKVDELRGKNKAELQAQLKDLKAELSLLRVAKVTGGAPNKLSKIKVVRTSIARVLTV 60
MARIKVD LRG+NKAELQAQLKDLKAELS+LRVA+VTGGAPNKLS IKV
Sbjct: 1 MARIKVDVLRGRNKAELQAQLKDLKAELSVLRVARVTGGAPNKLSNIKV----------- 49
Query: 61 ISQKQRAALREAY--KKKSLLPLDLRPKKTRAIRRRLTKHQ 99
R ALREAY KKKSLLPLDLRPKKT AIRRRLTKHQ
Sbjct: 50 -----RTALREAYKKKKKSLLPLDLRPKKTCAIRRRLTKHQ 85
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.318 0.130 0.336
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,218,264
Number of extensions: 97219
Number of successful extensions: 465
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 463
Number of HSP's successfully gapped: 3
Length of query: 123
Length of database: 17,035,801
Length adjustment: 88
Effective length of query: 35
Effective length of database: 12,440,969
Effective search space: 435433915
Effective search space used: 435433915
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 149 (62.0 bits)