BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os02g0437800 Os02g0437800|AK066001
         (567 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os02g0437800  Similar to Vacuolar protein-sorting protein 45...  1115   0.0  
Os06g0135900  Similar to Sec1p-like protein 2 (Fragment)           90   4e-18
Os03g0620800  Similar to SEC1-family transport protein SLY1 ...    67   3e-11

>Os02g0437800 Similar to Vacuolar protein-sorting protein 45 homolog (AtVPS45)
          Length = 567

 Score = 1115 bits (2884), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 545/567 (96%), Positives = 545/567 (96%)

Query: 1   MTLITLIRDYIDRMLHDIPGMKVLVLDPDTVGMVSVVYSQSDLLRKEVFLVETVDNASSS 60
           MTLITLIRDYIDRMLHDIPGMKVLVLDPDTVGMVSVVYSQSDLLRKEVFLVETVDNASSS
Sbjct: 1   MTLITLIRDYIDRMLHDIPGMKVLVLDPDTVGMVSVVYSQSDLLRKEVFLVETVDNASSS 60

Query: 61  RESMAHLKAVYFLRPSSDNVQKLRRHLAAPRFAEYHLFFSNVLKIPQIQVLADSDXXXXX 120
           RESMAHLKAVYFLRPSSDNVQKLRRHLAAPRFAEYHLFFSNVLKIPQIQVLADSD     
Sbjct: 61  RESMAHLKAVYFLRPSSDNVQKLRRHLAAPRFAEYHLFFSNVLKIPQIQVLADSDEQEVV 120

Query: 121 XXXXXFYADFCAIDPYHFTLNIRNNHVYMLPMVVDPPGMQSFCDRAVDGIASVFLALKRR 180
                FYADFCAIDPYHFTLNIRNNHVYMLPMVVDPPGMQSFCDRAVDGIASVFLALKRR
Sbjct: 121 QQVQEFYADFCAIDPYHFTLNIRNNHVYMLPMVVDPPGMQSFCDRAVDGIASVFLALKRR 180

Query: 181 PVIRYQRTSDVAKRIAQETARLMYEQESGLFDFRRTENSSLLLVIDRRDDPVTPLLNQWT 240
           PVIRYQRTSDVAKRIAQETARLMYEQESGLFDFRRTENSSLLLVIDRRDDPVTPLLNQWT
Sbjct: 181 PVIRYQRTSDVAKRIAQETARLMYEQESGLFDFRRTENSSLLLVIDRRDDPVTPLLNQWT 240

Query: 241 YQAMVHELIGIENNKVDLREYPNVPKDQKEVVLSSVQDEFFRANMFENFGDLGMNIKRMV 300
           YQAMVHELIGIENNKVDLREYPNVPKDQKEVVLSSVQDEFFRANMFENFGDLGMNIKRMV
Sbjct: 241 YQAMVHELIGIENNKVDLREYPNVPKDQKEVVLSSVQDEFFRANMFENFGDLGMNIKRMV 300

Query: 301 DDFQHLSKTSQNIQSISDMSKFLSNYPEYRKTHGNVTKHVALVSEMSRIVEERKIMLISQ 360
           DDFQHLSKTSQNIQSISDMSKFLSNYPEYRKTHGNVTKHVALVSEMSRIVEERKIMLISQ
Sbjct: 301 DDFQHLSKTSQNIQSISDMSKFLSNYPEYRKTHGNVTKHVALVSEMSRIVEERKIMLISQ 360

Query: 361 TEQELACTSGQAAAFEAVTSLLNNESVSDIDXXXXXXXXXXXXEKESPVQLMQLFNKLAS 420
           TEQELACTSGQAAAFEAVTSLLNNESVSDID            EKESPVQLMQLFNKLAS
Sbjct: 361 TEQELACTSGQAAAFEAVTSLLNNESVSDIDRLRLVLLYALRYEKESPVQLMQLFNKLAS 420

Query: 421 RSAKYKSGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLLFQT 480
           RSAKYKSGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLLFQT
Sbjct: 421 RSAKYKSGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLLFQT 480

Query: 481 MEGIVKARLRDADYPLVGNHFQQNRPQDVVLFIVGGTTYEEARSVALYNAANPGVRFFLG 540
           MEGIVKARLRDADYPLVGNHFQQNRPQDVVLFIVGGTTYEEARSVALYNAANPGVRFFLG
Sbjct: 481 MEGIVKARLRDADYPLVGNHFQQNRPQDVVLFIVGGTTYEEARSVALYNAANPGVRFFLG 540

Query: 541 GSVVLNSKRFLDDLGEAQRISKSSSLV 567
           GSVVLNSKRFLDDLGEAQRISKSSSLV
Sbjct: 541 GSVVLNSKRFLDDLGEAQRISKSSSLV 567



>Os06g0135900 Similar to Sec1p-like protein 2 (Fragment)
          Length = 522

 Score = 90.1 bits (222), Expect = 4e-18,   Method: Compositional matrix adjust.
 Identities = 95/409 (23%), Positives = 183/409 (44%), Gaps = 40/409 (9%)

Query: 12  DRMLHDI----------PGMKVLVLDPDTVGMVSVVYSQSDLLRKEVFLVETVDNASSSR 61
           DR+L D+             KVL++D  TV +++     +++    + LVE   +    R
Sbjct: 24  DRILKDLLQPDKDKETKSSWKVLIMDKFTVRIMAYACKMAEITDAGISLVE---DLFKRR 80

Query: 62  ESMAHLKAVYFLRPSSDNVQKLRRHLA--APRFAEYHLFFSNVLKIPQIQVLA-DSDXXX 118
           E M  + A+YFL+P  +NV  L   ++   P + + ++FFS+ +    +  +  DS    
Sbjct: 81  EPMPSMDAIYFLQPLKENVIMLLSDMSGRCPLYRKAYIFFSSPIPKELVSYIKNDSSVIP 140

Query: 119 XXXXXXXFYADFCAIDPYHFTLNIRNNHVYMLPMVVDPPGMQSFCDRAVDGIASVFLALK 178
                     +F AID   FT +       +     +              IA+ F +LK
Sbjct: 141 RIGALREMNLEFFAIDMQGFTTDHDMAFTDLYSAQHNSKKFNDTISTMATRIATTFASLK 200

Query: 179 RRPVIRYQ--RTSD---------VAKRIAQETARLMYEQESGLFDFRRTENSSLLLVIDR 227
             P +RY+  + +D         V K +A     ++ + +S + +F + E   LL+V DR
Sbjct: 201 EFPCVRYRAPKGTDPMTTPKFDMVPKWLATAVWDIVSKYKSTIPEFPQKETCELLIV-DR 259

Query: 228 RDDPVTPLLNQWTYQAMVHELIGIENNK--VDLREYPNVPKDQKEVVLSSVQDEFFRANM 285
             D + P++++WTY AM H+L+ ++  K   ++ +  + P ++KE +L    D  +    
Sbjct: 260 PIDQIAPVIHEWTYDAMCHDLLEMDGQKYIYEVSKAGSEP-ERKEALLED-HDPLWVELR 317

Query: 286 FENFGDLGMNIKRMVDDFQHLSKTSQ------NIQSISDMSKFLSNYPEYRKTHGNVTKH 339
             +  D    +   +++F   +K +Q         S  D+ K +   P+Y +    +T H
Sbjct: 318 HIHIADASERLYDKMNNFVSKNKAAQLHSRDGGEISTKDLQKIVQALPQYGEQVEKLTLH 377

Query: 340 VALVSEMSRIVEERKIMLISQTEQELACTSGQAAAFEAVTSLLNNESVS 388
           + +  ++++ + E  +  I Q EQ+L    G AAA E ++ L + + +S
Sbjct: 378 IEIAGKINKFIREYGLRDIGQVEQDLVF--GDAAAKEVISILRSKQDMS 424



>Os03g0620800 Similar to SEC1-family transport protein SLY1 (AtSLY1)
          Length = 623

 Score = 67.4 bits (163), Expect = 3e-11,   Method: Compositional matrix adjust.
 Identities = 83/356 (23%), Positives = 147/356 (41%), Gaps = 48/356 (13%)

Query: 22  KVLVLDPDTVGMVSVVYSQSDLLRKEVFLVETVDNASSSRESMAHLKAVYFLRPSSDNVQ 81
           K+LV+D   V +++ V    +L R  V L   +D A   R+ +    AVY LRP++ NV 
Sbjct: 44  KILVMDSPCVALLAPVLRVGELRRHGVTLHLNIDKA---RQQVPDAPAVYLLRPTAANVD 100

Query: 82  KLRRHLAAPRFAEYHLFFSNVLKIPQIQVLADSDXXXXXXXXXXF----YADFCAIDPYH 137
           ++    AA  +A +HL FS  +    ++ LA +                Y DF  ++   
Sbjct: 101 RVAADAAAGLYASFHLNFSTCVPRALLERLASATAASRSAHRVARVADQYLDFVCLEEGL 160

Query: 138 FTLNIRNNHVYMLPMVVDPPGMQSFCDRAVDGIA----SVFLALKRRPVIRYQRTSDVAK 193
           F+L     +V +     DP   ++     VD IA     V   L   PVIR         
Sbjct: 161 FSLAQPRAYVAL----NDPAAAEADITALVDAIALGLFCVVATLGAVPVIRCAGGGPAEM 216

Query: 194 RIAQETARL-----------MYEQESGLFDFRRTENSSLLLVIDRRDDPVTPLLNQWTYQ 242
             A   ARL                + +  F+R     LL + DR  +    + + W+Y+
Sbjct: 217 VAAALDARLRDHLIAKPNLFTEAASTAVASFQR----PLLCLFDRNFELSVGIQHDWSYR 272

Query: 243 AMVHELIGIENNKVDLREYPNVPKDQKEVVLSSVQDEFFRANMFENFGDLGMNIKRMVDD 302
            +VH+++G+++NK+ L E  ++             D F+ AN +  F  +   I+     
Sbjct: 273 PLVHDVLGLKSNKLKLPEKYDLDD----------TDPFWVANSWLQFPKVAEEIE----- 317

Query: 303 FQHLSKTSQNIQSISDMSKFLSNYPEYRKTH--GNVTKHVALVSEMSRIVEERKIM 356
              L+K  Q++  ++  +    +  E+  T   GN    +  V+ +  + E +K++
Sbjct: 318 -AQLAKYKQDVDEVNQRTGGGRDGVEFDGTDLIGNTRHLMNAVNSLPELTERKKMI 372



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.135    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 16,173,617
Number of extensions: 610354
Number of successful extensions: 1262
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 1260
Number of HSP's successfully gapped: 3
Length of query: 567
Length of database: 17,035,801
Length adjustment: 106
Effective length of query: 461
Effective length of database: 11,501,117
Effective search space: 5302014937
Effective search space used: 5302014937
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 159 (65.9 bits)