BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os02g0280500 Os02g0280500|AK058486
         (82 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os02g0280500  Similar to Glyoxalase I                             166   5e-42
Os05g0230900  Similar to Glyoxalase I                             144   1e-35
Os08g0191700  Similar to Lactoylglutathione lyase (EC 4.4.1....   129   4e-31

>Os02g0280500 Similar to Glyoxalase I
          Length = 82

 Score =  166 bits (419), Expect = 5e-42,   Method: Compositional matrix adjust.
 Identities = 82/82 (100%), Positives = 82/82 (100%)

Query: 1  AVLELTYNYGVTEYDKGNAYAQIAIGTDDVYKTAEVVKLFGGQVVREPGPLPGINTKITS 60
          AVLELTYNYGVTEYDKGNAYAQIAIGTDDVYKTAEVVKLFGGQVVREPGPLPGINTKITS
Sbjct: 1  AVLELTYNYGVTEYDKGNAYAQIAIGTDDVYKTAEVVKLFGGQVVREPGPLPGINTKITS 60

Query: 61 ILDPDGWKSVFVDNIDFAKELE 82
          ILDPDGWKSVFVDNIDFAKELE
Sbjct: 61 ILDPDGWKSVFVDNIDFAKELE 82



>Os05g0230900 Similar to Glyoxalase I
          Length = 291

 Score =  144 bits (364), Expect = 1e-35,   Method: Compositional matrix adjust.
 Identities = 68/82 (82%), Positives = 74/82 (90%)

Query: 1   AVLELTYNYGVTEYDKGNAYAQIAIGTDDVYKTAEVVKLFGGQVVREPGPLPGINTKITS 60
           AVLELTYNYGV EYDKGNAYAQIAI TDDVYKTAEV++  GGQ+ REPGPLPGINTKIT+
Sbjct: 209 AVLELTYNYGVKEYDKGNAYAQIAISTDDVYKTAEVIRQNGGQITREPGPLPGINTKITA 268

Query: 61  ILDPDGWKSVFVDNIDFAKELE 82
             DPDGWK+VFVDN+DF KELE
Sbjct: 269 CTDPDGWKTVFVDNVDFLKELE 290




 Score = 72.0 bits (175), Expect = 8e-14,   Method: Compositional matrix adjust.
 Identities = 35/73 (47%), Positives = 46/73 (63%)

Query: 2   VLELTYNYGVTEYDKGNAYAQIAIGTDDVYKTAEVVKLFGGQVVREPGPLPGINTKITSI 61
           V+ELTYNYGV  YD G A+    I  +DV KT +++K  GG V REPGP+ G  + I  I
Sbjct: 80  VVELTYNYGVESYDIGTAFGHFGIAVEDVAKTVDLIKAKGGTVTREPGPVKGGKSVIAFI 139

Query: 62  LDPDGWKSVFVDN 74
            DPDG+K   ++ 
Sbjct: 140 EDPDGYKFELIER 152



>Os08g0191700 Similar to Lactoylglutathione lyase (EC 4.4.1.5) (Methylglyoxalase)
           (Aldoketomutase) (Glyoxalase I) (Glx I) (Ketone-aldehyde
           mutase) (S-D- lactoylglutathione methylglyoxal lyase)
          Length = 291

 Score =  129 bits (325), Expect = 4e-31,   Method: Compositional matrix adjust.
 Identities = 61/85 (71%), Positives = 72/85 (84%), Gaps = 4/85 (4%)

Query: 2   VLELTYNYGVTEYDKGNAYAQIAIGTDDVYKTAEVVKL----FGGQVVREPGPLPGINTK 57
           V+ELTYNYGVTEY KGNAYAQ+AIGT+DVYK+AE V+L     GG+++R+PGPLPG+NTK
Sbjct: 207 VIELTYNYGVTEYTKGNAYAQVAIGTEDVYKSAEAVELVTKELGGKILRQPGPLPGLNTK 266

Query: 58  ITSILDPDGWKSVFVDNIDFAKELE 82
           I S LDPDGWK V VDN DF KEL+
Sbjct: 267 IASFLDPDGWKVVLVDNADFLKELQ 291




 Score = 66.2 bits (160), Expect = 5e-12,   Method: Compositional matrix adjust.
 Identities = 34/67 (50%), Positives = 43/67 (64%), Gaps = 1/67 (1%)

Query: 2   VLELTYNYGVTEYDKGNAYAQIAIGTDDVYKTAEVVK-LFGGQVVREPGPLPGINTKITS 60
            LELTYNYGV +YD G  +   AI T+DVYK AE +K     ++ REPGP+ G +T I  
Sbjct: 76  ALELTYNYGVDKYDIGAGFGHFAIATEDVYKLAEKIKSSCCCKITREPGPVKGGSTVIAF 135

Query: 61  ILDPDGW 67
             DPDG+
Sbjct: 136 AQDPDGY 142



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.315    0.138    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,878,856
Number of extensions: 100632
Number of successful extensions: 147
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 145
Number of HSP's successfully gapped: 5
Length of query: 82
Length of database: 17,035,801
Length adjustment: 52
Effective length of query: 30
Effective length of database: 14,320,673
Effective search space: 429620190
Effective search space used: 429620190
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 149 (62.0 bits)