BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os02g0254700 Os02g0254700|AK065153
         (193 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os02g0254700  Similar to Aminopropyl transferase                  372   e-104
Os06g0528600  Aminopropyl transferase                             330   4e-91
Os07g0408700  Similar to Spermidine synthase 2 (EC 2.5.1.16)...   238   2e-63

>Os02g0254700 Similar to Aminopropyl transferase
          Length = 193

 Score =  372 bits (955), Expect = e-104,   Method: Compositional matrix adjust.
 Identities = 179/193 (92%), Positives = 179/193 (92%)

Query: 1   DPRVRLHVGDAVEFLRNAPEGTYDAIIVDSSDPIGPAQELVEKPFFDTIARALRPGGVLC 60
           DPRVRLHVGDAVEFLRNAPEGTYDAIIVDSSDPIGPAQELVEKPFFDTIARALRPGGVLC
Sbjct: 1   DPRVRLHVGDAVEFLRNAPEGTYDAIIVDSSDPIGPAQELVEKPFFDTIARALRPGGVLC 60

Query: 61  NQAESMWLHTHLIQDMLSICRETFKGSVHYAWTSVPTYPSGVIGFLLCAKEGPPVNFLTP 120
           NQAESMWLHTHLIQDMLSICRETFKGSVHYAWTSVPTYPSGVIGFLLCAKEGPPVNFLTP
Sbjct: 61  NQAESMWLHTHLIQDMLSICRETFKGSVHYAWTSVPTYPSGVIGFLLCAKEGPPVNFLTP 120

Query: 121 ANPIEKLEGAMEAGRDIRFYNSEMHRAAFVLPTFARRELEAYCXXXXXXXXXXXXXXPLK 180
           ANPIEKLEGAMEAGRDIRFYNSEMHRAAFVLPTFARRELEAYC              PLK
Sbjct: 121 ANPIEKLEGAMEAGRDIRFYNSEMHRAAFVLPTFARRELEAYCTTVEREQQEETTAEPLK 180

Query: 181 MNIMPNSEILTAS 193
           MNIMPNSEILTAS
Sbjct: 181 MNIMPNSEILTAS 193



>Os06g0528600 Aminopropyl transferase
          Length = 387

 Score =  330 bits (846), Expect = 4e-91,   Method: Compositional matrix adjust.
 Identities = 160/194 (82%), Positives = 169/194 (87%), Gaps = 1/194 (0%)

Query: 1   DPRVRLHVGDAVEFLRNAPEGTYDAIIVDSSDPIGPAQELVEKPFFDTIARALRPGGVLC 60
           DPRV+LHVGDAV+FLRNAPEG YDAIIVDSSDPIGPAQELVEKPFF TIARAL+PGGVLC
Sbjct: 194 DPRVQLHVGDAVDFLRNAPEGKYDAIIVDSSDPIGPAQELVEKPFFQTIARALKPGGVLC 253

Query: 61  NQAESMWLHTHLIQDMLSICRETFKGSVHYAWTSVPTYPSGVIGFLLCAKEGPPVNFLTP 120
           NQAESMWLHTHLIQDMLSICRETFKG+VHYAWTSVPTYPSGVIGFLLCAKEGP VNFL+P
Sbjct: 254 NQAESMWLHTHLIQDMLSICRETFKGAVHYAWTSVPTYPSGVIGFLLCAKEGPAVNFLSP 313

Query: 121 ANPIEKLEGAMEAGRDIRFYNSEMHRAAFVLPTFARRELEAY-CXXXXXXXXXXXXXXPL 179
            NPIEKLEGAMEAGR+IRFYNSE+HRAAFVLPTF RRELE++                P 
Sbjct: 314 VNPIEKLEGAMEAGREIRFYNSEVHRAAFVLPTFVRRELESHNTCAEKDKSETKPVAKPK 373

Query: 180 KMNIMPNSEILTAS 193
           KM IMPNS I TAS
Sbjct: 374 KMKIMPNSAIPTAS 387



>Os07g0408700 Similar to Spermidine synthase 2 (EC 2.5.1.16) (Putrescine
           aminopropyltransferase 2) (SPDSY 2)
          Length = 323

 Score =  238 bits (608), Expect = 2e-63,   Method: Compositional matrix adjust.
 Identities = 105/157 (66%), Positives = 132/157 (84%)

Query: 1   DPRVRLHVGDAVEFLRNAPEGTYDAIIVDSSDPIGPAQELVEKPFFDTIARALRPGGVLC 60
           DPRV LH+GD V FL+NAPEGTYDA+IVDSSDPIGPAQEL EKPFF ++ARALRPGGV+C
Sbjct: 160 DPRVSLHIGDGVAFLKNAPEGTYDAVIVDSSDPIGPAQELFEKPFFQSVARALRPGGVVC 219

Query: 61  NQAESMWLHTHLIQDMLSICRETFKGSVHYAWTSVPTYPSGVIGFLLCAKEGPPVNFLTP 120
            QAES+WLH H+I+D+++ CR+ FKGSV+YAWT+VPTYPSGVIGF+LC+ EGP V+F  P
Sbjct: 220 TQAESIWLHMHIIEDIVANCRQVFKGSVNYAWTTVPTYPSGVIGFMLCSTEGPTVDFQHP 279

Query: 121 ANPIEKLEGAMEAGRDIRFYNSEMHRAAFVLPTFARR 157
              IE  E + ++   ++FYNSE+H A+F LP+FA+R
Sbjct: 280 IFNIEDNEFSTKSKGPLKFYNSEIHSASFCLPSFAKR 316



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.138    0.426 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,468,891
Number of extensions: 265247
Number of successful extensions: 412
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 411
Number of HSP's successfully gapped: 3
Length of query: 193
Length of database: 17,035,801
Length adjustment: 95
Effective length of query: 98
Effective length of database: 12,075,471
Effective search space: 1183396158
Effective search space used: 1183396158
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 153 (63.5 bits)