BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os02g0237100 Os02g0237100|AK073410
         (344 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os02g0237100  Similar to Spermine synthase                        722   0.0  
Os07g0408700  Similar to Spermidine synthase 2 (EC 2.5.1.16)...   123   2e-28
Os06g0528600  Aminopropyl transferase                             110   1e-24

>Os02g0237100 Similar to Spermine synthase
          Length = 344

 Score =  722 bits (1864), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 344/344 (100%), Positives = 344/344 (100%)

Query: 1   MVGAVQEGIVREMNGGFEVMSGYDRSSMAPKQQQREEESKWYEEEIDDDLKLCYALNSVL 60
           MVGAVQEGIVREMNGGFEVMSGYDRSSMAPKQQQREEESKWYEEEIDDDLKLCYALNSVL
Sbjct: 1   MVGAVQEGIVREMNGGFEVMSGYDRSSMAPKQQQREEESKWYEEEIDDDLKLCYALNSVL 60

Query: 61  HRGASKYQEIALIDTKHFGKALIIDGKMQSTEVDEFIYHESLIHPPLLFHPNPKTVFIMG 120
           HRGASKYQEIALIDTKHFGKALIIDGKMQSTEVDEFIYHESLIHPPLLFHPNPKTVFIMG
Sbjct: 61  HRGASKYQEIALIDTKHFGKALIIDGKMQSTEVDEFIYHESLIHPPLLFHPNPKTVFIMG 120

Query: 121 GGEGSAAREVLRHNTVHRVVMCDIDQEVVDFCRTYLTVNWDAFASDKLCLIINDARAELE 180
           GGEGSAAREVLRHNTVHRVVMCDIDQEVVDFCRTYLTVNWDAFASDKLCLIINDARAELE
Sbjct: 121 GGEGSAAREVLRHNTVHRVVMCDIDQEVVDFCRTYLTVNWDAFASDKLCLIINDARAELE 180

Query: 181 KSREKFDVIVGDLADPVEGGPCYQLYTKSFYQHIVKPKLNDRGVFVTQAGPAGVLTHKEV 240
           KSREKFDVIVGDLADPVEGGPCYQLYTKSFYQHIVKPKLNDRGVFVTQAGPAGVLTHKEV
Sbjct: 181 KSREKFDVIVGDLADPVEGGPCYQLYTKSFYQHIVKPKLNDRGVFVTQAGPAGVLTHKEV 240

Query: 241 FSSIYNTLRHVFKYVKAYTAHVPSFADTWGWVMASDYPFSMNAQQINERIKERIDGELVY 300
           FSSIYNTLRHVFKYVKAYTAHVPSFADTWGWVMASDYPFSMNAQQINERIKERIDGELVY
Sbjct: 241 FSSIYNTLRHVFKYVKAYTAHVPSFADTWGWVMASDYPFSMNAQQINERIKERIDGELVY 300

Query: 301 LSGESLISSTILNKSVYQSLLNETHVYTEDDARFIYGHGRARCA 344
           LSGESLISSTILNKSVYQSLLNETHVYTEDDARFIYGHGRARCA
Sbjct: 301 LSGESLISSTILNKSVYQSLLNETHVYTEDDARFIYGHGRARCA 344



>Os07g0408700 Similar to Spermidine synthase 2 (EC 2.5.1.16) (Putrescine
           aminopropyltransferase 2) (SPDSY 2)
          Length = 323

 Score =  123 bits (309), Expect = 2e-28,   Method: Compositional matrix adjust.
 Identities = 69/199 (34%), Positives = 108/199 (54%), Gaps = 6/199 (3%)

Query: 56  LNSVLHRGASKYQEIALIDTKHFGKALIIDGKMQSTEVDEFIYHESLIHPPLLFHPNPKT 115
           +  VL +G S YQ + +  +  +GK L++DG +Q TE DE  Y E + H PL    +PK 
Sbjct: 51  VEKVLFQGKSDYQNVMVFQSSTYGKVLVLDGVIQVTERDECAYQEMITHLPLCSIKDPKK 110

Query: 116 VFIMGGGEGSAAREVLRHNTVHRVVMCDIDQEVVDFCRTYLTVNWDAFASDKLCLIINDA 175
           V ++GGG+G   REV RH++V ++ +C+ID+ VVD  + +       F   ++ L I D 
Sbjct: 111 VLVIGGGDGGVLREVSRHSSVEQIDICEIDKMVVDVSKQFFPHLAVGFEDPRVSLHIGDG 170

Query: 176 RAELEKSRE-KFDVIVGDLADPVEGGPCYQLYTKSFYQHIVKPKLNDRGVFVTQAGPAGV 234
            A L+ + E  +D ++ D +DP+  GP  +L+ K F+Q + +  L   GV  TQA    +
Sbjct: 171 VAFLKNAPEGTYDAVIVDSSDPI--GPAQELFEKPFFQSVAR-ALRPGGVVCTQA--ESI 225

Query: 235 LTHKEVFSSIYNTLRHVFK 253
             H  +   I    R VFK
Sbjct: 226 WLHMHIIEDIVANCRQVFK 244



>Os06g0528600 Aminopropyl transferase
          Length = 387

 Score =  110 bits (275), Expect = 1e-24,   Method: Compositional matrix adjust.
 Identities = 72/212 (33%), Positives = 115/212 (54%), Gaps = 7/212 (3%)

Query: 56  LNSVLHRGASKYQEIALIDTKHFGKALIIDGKMQSTEVDEFIYHESLIHPPLLFHPNPKT 115
           + ++L++G S YQEI + ++  +G  L++DG +Q TE DE  Y E + H PL   P+PK+
Sbjct: 85  VENILYQGKSPYQEILVFESSTYGNVLVLDGIVQLTEKDECAYQEMVTHLPLCSIPSPKS 144

Query: 116 VFIMGGGEGSAAREVLRHNTVHRVVMCDIDQEVVDFCRTYLTVNWDAFASDKLCLIINDA 175
           V ++GGG+G   RE+ RH +V  + +C+IDQ V+D C+ +       F   ++ L + DA
Sbjct: 145 VLVVGGGDGGVLREIARHASVENIDICEIDQLVIDVCKDFFPQLSVGFKDPRVQLHVGDA 204

Query: 176 RAELEKSRE-KFDVIVGDLADPVEGGPCYQLYTKSFYQHIVKPKLNDRGVFVTQAGPAGV 234
              L  + E K+D I+ D +DP+  GP  +L  K F+Q I +  L   GV   QA    +
Sbjct: 205 VDFLRNAPEGKYDAIIVDSSDPI--GPAQELVEKPFFQTIAR-ALKPGGVLCNQA--ESM 259

Query: 235 LTHKEVFSSIYNTLRHVFKYVKAYT-AHVPSF 265
             H  +   + +  R  FK    Y    VP++
Sbjct: 260 WLHTHLIQDMLSICRETFKGAVHYAWTSVPTY 291



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.136    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 12,204,597
Number of extensions: 515642
Number of successful extensions: 1275
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 1274
Number of HSP's successfully gapped: 3
Length of query: 344
Length of database: 17,035,801
Length adjustment: 102
Effective length of query: 242
Effective length of database: 11,709,973
Effective search space: 2833813466
Effective search space used: 2833813466
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 156 (64.7 bits)