BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os02g0193600 Os02g0193600|AK060499
         (398 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os02g0193600  Mad3/BUB1 homology region 1 domain containing ...   722   0.0  
Os04g0454900  Hypothetical protein                                114   1e-25
Os12g0125300  Mad3/BUB1 homology region 1 domain containing ...    94   3e-19

>Os02g0193600 Mad3/BUB1 homology region 1 domain containing protein
          Length = 398

 Score =  722 bits (1863), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 350/379 (92%), Positives = 350/379 (92%)

Query: 20  LDKETLMLMGCGDXXXXXXCAEWETFKENVRPLKRGRNVGLLNRALKXXXXXXXXXXXXX 79
           LDKETLMLMGCGD      CAEWETFKENVRPLKRGRNVGLLNRALK             
Sbjct: 20  LDKETLMLMGCGDAPPAAPCAEWETFKENVRPLKRGRNVGLLNRALKAHADPAQRAALLA 79

Query: 80  XXXKMIEAIDEYSGEDPLQPWIDCIKWVQESFPTGGDCSGLVVIYEQCVRAFWHDDRYKN 139
              KMIEAIDEYSGEDPLQPWIDCIKWVQESFPTGGDCSGLVVIYEQCVRAFWHDDRYKN
Sbjct: 80  ARRKMIEAIDEYSGEDPLQPWIDCIKWVQESFPTGGDCSGLVVIYEQCVRAFWHDDRYKN 139

Query: 140 DLRYLKVWLEYAGNCADSEVIFRFLEANQIGQSHTNYYLSYASVMESKNKLKKANEIFNL 199
           DLRYLKVWLEYAGNCADSEVIFRFLEANQIGQSHTNYYLSYASVMESKNKLKKANEIFNL
Sbjct: 140 DLRYLKVWLEYAGNCADSEVIFRFLEANQIGQSHTNYYLSYASVMESKNKLKKANEIFNL 199

Query: 200 GIARKAKPVEKLETTYRAFLQRSSKKKAHPEXXXXXXXHPVRNFGTVLNRGEIRGQHAEN 259
           GIARKAKPVEKLETTYRAFLQRSSKKKAHPE       HPVRNFGTVLNRGEIRGQHAEN
Sbjct: 200 GIARKAKPVEKLETTYRAFLQRSSKKKAHPEDDTTTDDHPVRNFGTVLNRGEIRGQHAEN 259

Query: 260 SHLVKTLQRVDVNRPLAVYTDENSLPSHGLARTRSNNTAWQTLGSQADRNKENNMRPAKW 319
           SHLVKTLQRVDVNRPLAVYTDENSLPSHGLARTRSNNTAWQTLGSQADRNKENNMRPAKW
Sbjct: 260 SHLVKTLQRVDVNRPLAVYTDENSLPSHGLARTRSNNTAWQTLGSQADRNKENNMRPAKW 319

Query: 320 TSHKIPQKVGSRAAVQPTRVSSIEVFVDDECAEEPVPQVPKSTKPSILKLRQATSRNLKQ 379
           TSHKIPQKVGSRAAVQPTRVSSIEVFVDDECAEEPVPQVPKSTKPSILKLRQATSRNLKQ
Sbjct: 320 TSHKIPQKVGSRAAVQPTRVSSIEVFVDDECAEEPVPQVPKSTKPSILKLRQATSRNLKQ 379

Query: 380 ETELLMENPLRNFPLTSLR 398
           ETELLMENPLRNFPLTSLR
Sbjct: 380 ETELLMENPLRNFPLTSLR 398



>Os04g0454900 Hypothetical protein
          Length = 291

 Score =  114 bits (286), Expect = 1e-25,   Method: Compositional matrix adjust.
 Identities = 56/74 (75%), Positives = 63/74 (85%)

Query: 324 IPQKVGSRAAVQPTRVSSIEVFVDDECAEEPVPQVPKSTKPSILKLRQATSRNLKQETEL 383
           I QKVGSRAA+    V+ IEVFVDDECA +P PQVPKS+KPSI+KLRQATSRNLK+E EL
Sbjct: 74  ICQKVGSRAAILQNLVNPIEVFVDDECAGKPAPQVPKSSKPSIMKLRQATSRNLKREIEL 133

Query: 384 LMENPLRNFPLTSL 397
           LMENP RNFPLT+ 
Sbjct: 134 LMENPQRNFPLTNF 147



>Os12g0125300 Mad3/BUB1 homology region 1 domain containing protein
          Length = 622

 Score = 93.6 bits (231), Expect = 3e-19,   Method: Compositional matrix adjust.
 Identities = 40/138 (28%), Positives = 78/138 (56%)

Query: 84  MIEAIDEYSGEDPLQPWIDCIKWVQESFPTGGDCSGLVVIYEQCVRAFWHDDRYKNDLRY 143
           ++  I  YSG DPL+PW+  ++ ++ + P     + L    ++C + F  D RY++D RY
Sbjct: 24  VVGDIRSYSGSDPLRPWLRGMRKMEAALPPATLRAKLPRFLQKCAQEFQDDARYRDDSRY 83

Query: 144 LKVWLEYAGNCADSEVIFRFLEANQIGQSHTNYYLSYASVMESKNKLKKANEIFNLGIAR 203
           L+VW++      D++ + + +E N+IG   + +Y++YA   E   + + A  ++ LG   
Sbjct: 84  LRVWIQLMDYVKDAKPLLKKMEKNRIGLKRSAFYMAYAVYYEKHKRFEDAENMYRLGTQN 143

Query: 204 KAKPVEKLETTYRAFLQR 221
            A+PV +L+  +  F++R
Sbjct: 144 LAEPVGELQKAHEQFIRR 161



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.316    0.132    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 11,831,752
Number of extensions: 464730
Number of successful extensions: 1084
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 1083
Number of HSP's successfully gapped: 3
Length of query: 398
Length of database: 17,035,801
Length adjustment: 103
Effective length of query: 295
Effective length of database: 11,657,759
Effective search space: 3439038905
Effective search space used: 3439038905
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 157 (65.1 bits)