BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os02g0130000 Os02g0130000|AK106464
         (131 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os02g0130000  Similar to SWIb domain-containing protein (Fra...   219   3e-58
Os09g0133000  SWIB/MDM2 domain containing protein                  74   4e-14
Os08g0502800  MDM2 oncoprotein family protein                      70   5e-13

>Os02g0130000 Similar to SWIb domain-containing protein (Fragment)
          Length = 131

 Score =  219 bits (559), Expect = 3e-58,   Method: Compositional matrix adjust.
 Identities = 111/131 (84%), Positives = 111/131 (84%)

Query: 1   MAAAARVFRGCRFLMXXXXXXXXXXXXXXXXXXXXKVTKTEAAQAKEKRGIMQPVPVSDA 60
           MAAAARVFRGCRFLM                    KVTKTEAAQAKEKRGIMQPVPVSDA
Sbjct: 1   MAAAARVFRGCRFLMAAAGGKRPASASASASAAGAKVTKTEAAQAKEKRGIMQPVPVSDA 60

Query: 61  LSRFAGGAPEMSRAGAVKLIWNHIKANGLQNPANKREINCDDKLKSLFAGKDKVGMMEIA 120
           LSRFAGGAPEMSRAGAVKLIWNHIKANGLQNPANKREINCDDKLKSLFAGKDKVGMMEIA
Sbjct: 61  LSRFAGGAPEMSRAGAVKLIWNHIKANGLQNPANKREINCDDKLKSLFAGKDKVGMMEIA 120

Query: 121 KLLSPHFIKAN 131
           KLLSPHFIKAN
Sbjct: 121 KLLSPHFIKAN 131



>Os09g0133000 SWIB/MDM2 domain containing protein
          Length = 102

 Score = 73.6 bits (179), Expect = 4e-14,   Method: Compositional matrix adjust.
 Identities = 33/75 (44%), Positives = 48/75 (64%)

Query: 55  VPVSDALSRFAGGAPEMSRAGAVKLIWNHIKANGLQNPANKREINCDDKLKSLFAGKDKV 114
           V +  AL  FAGG  +MS       +W+HIK+N LQ+P+N+  +NCDDKLK++  G+ KV
Sbjct: 24  VNLPTALREFAGGQSQMSHLSFFLRVWSHIKSNNLQDPSNRNIVNCDDKLKTVLLGRSKV 83

Query: 115 GMMEIAKLLSPHFIK 129
            + E+  L+  HF K
Sbjct: 84  ELSELPMLVKLHFPK 98



>Os08g0502800 MDM2 oncoprotein family protein
          Length = 406

 Score = 69.7 bits (169), Expect = 5e-13,   Method: Compositional matrix adjust.
 Identities = 36/77 (46%), Positives = 51/77 (66%), Gaps = 2/77 (2%)

Query: 54  PVPVSDALSRFAG-GAPEMSRAGAVKLIWNHIKANGLQNPANKREINCDDKLKSLFAGKD 112
           P+ +SDAL+ F G G  EM  + AVK +W+HIK+N L++PAN   I CD KLK LF G +
Sbjct: 326 PIILSDALASFFGTGEKEMPSSEAVKRVWDHIKSNNLEDPANPTMILCDSKLKQLF-GCE 384

Query: 113 KVGMMEIAKLLSPHFIK 129
            +  + +++LLS H  K
Sbjct: 385 SLTAVSVSELLSQHLFK 401



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.132    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,613,671
Number of extensions: 116026
Number of successful extensions: 271
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 270
Number of HSP's successfully gapped: 3
Length of query: 131
Length of database: 17,035,801
Length adjustment: 89
Effective length of query: 42
Effective length of database: 12,388,755
Effective search space: 520327710
Effective search space used: 520327710
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 150 (62.4 bits)