BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os02g0125100 Os02g0125100|AK101889
         (514 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os02g0125100  Cis-homoaconitase family protein                    974   0.0  
Os06g0303400  Similar to Aconitate hydratase, cytoplasmic (E...    74   3e-13
Os03g0136900  Similar to Aconitate hydratase, cytoplasmic (E...    72   1e-12

>Os02g0125100 Cis-homoaconitase family protein
          Length = 514

 Score =  974 bits (2519), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 472/514 (91%), Positives = 472/514 (91%)

Query: 1   MASISXXXXXXXXXXXXXHKNELAAAAVAPSQQQLQXXXXXXXXXXXXXXXXXXXXXXXX 60
           MASIS             HKNELAAAAVAPSQQQLQ                        
Sbjct: 1   MASISAASPVAGKAAAFAHKNELAAAAVAPSQQQLQRRVSGRRARSGRVRAVATPARAPR 60

Query: 61  XXXXXGSVKSAMTMTEKILARASERASLEPGENVWVDVDVLMTHDVCGPGTIGIFKREFG 120
                GSVKSAMTMTEKILARASERASLEPGENVWVDVDVLMTHDVCGPGTIGIFKREFG
Sbjct: 61  APSSTGSVKSAMTMTEKILARASERASLEPGENVWVDVDVLMTHDVCGPGTIGIFKREFG 120

Query: 121 EDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCMEQNIKYFYDIKDLSNFKANPDY 180
           EDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCMEQNIKYFYDIKDLSNFKANPDY
Sbjct: 121 EDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCMEQNIKYFYDIKDLSNFKANPDY 180

Query: 181 KGVCHVALAQEGHCRPGEVLLGTDSHTCNAGAFGQFATGIGNTDAGFVMGTGKALLKVPP 240
           KGVCHVALAQEGHCRPGEVLLGTDSHTCNAGAFGQFATGIGNTDAGFVMGTGKALLKVPP
Sbjct: 181 KGVCHVALAQEGHCRPGEVLLGTDSHTCNAGAFGQFATGIGNTDAGFVMGTGKALLKVPP 240

Query: 241 TIRFVLDGEMPPYLLAKDLILQIIGEISVSGATYKSMEFVGSTVESLNMEERMTLCNMVI 300
           TIRFVLDGEMPPYLLAKDLILQIIGEISVSGATYKSMEFVGSTVESLNMEERMTLCNMVI
Sbjct: 241 TIRFVLDGEMPPYLLAKDLILQIIGEISVSGATYKSMEFVGSTVESLNMEERMTLCNMVI 300

Query: 301 EAGGKNGVVPADQTTFNYLEGKTSVEYEPVYSDAQARFVSDYRFDVSKLEPVIAKPHSPD 360
           EAGGKNGVVPADQTTFNYLEGKTSVEYEPVYSDAQARFVSDYRFDVSKLEPVIAKPHSPD
Sbjct: 301 EAGGKNGVVPADQTTFNYLEGKTSVEYEPVYSDAQARFVSDYRFDVSKLEPVIAKPHSPD 360

Query: 361 NRALARECKDVKIDRVYIGSCTGGKTEDFFAAAKVFLASGKKVKVPTFLVPATQKVWMDI 420
           NRALARECKDVKIDRVYIGSCTGGKTEDFFAAAKVFLASGKKVKVPTFLVPATQKVWMDI
Sbjct: 361 NRALARECKDVKIDRVYIGSCTGGKTEDFFAAAKVFLASGKKVKVPTFLVPATQKVWMDI 420

Query: 421 YSIPVPGAGGKTCSQIFEEAGCDTPASPSCGACLGGPRDTYARMNEPMVCVSTTNRNFPG 480
           YSIPVPGAGGKTCSQIFEEAGCDTPASPSCGACLGGPRDTYARMNEPMVCVSTTNRNFPG
Sbjct: 421 YSIPVPGAGGKTCSQIFEEAGCDTPASPSCGACLGGPRDTYARMNEPMVCVSTTNRNFPG 480

Query: 481 RMGHKEGQIYLASPFTAAASALTGYVTDPRDFLM 514
           RMGHKEGQIYLASPFTAAASALTGYVTDPRDFLM
Sbjct: 481 RMGHKEGQIYLASPFTAAASALTGYVTDPRDFLM 514



>Os06g0303400 Similar to Aconitate hydratase, cytoplasmic (EC 4.2.1.3) (Citrate
           hydro-lyase) (Aconitase)
          Length = 766

 Score = 73.6 bits (179), Expect = 3e-13,   Method: Compositional matrix adjust.
 Identities = 69/268 (25%), Positives = 110/268 (41%), Gaps = 30/268 (11%)

Query: 131 VVIIPDHYIFTSDERANRNVDILRDFCMEQNIKYFYDIKDLSN----FKANPDYKGVCH- 185
           V ++ DH +     R++  +D   +   ++N + F  +K  S      +  P   G+ H 
Sbjct: 67  VDVVIDHAVRVDVVRSHDALDKNMELEFDRNKERFGFLKWASTAFHKMQVFPPGSGIVHQ 126

Query: 186 --------VALAQEGHCRPGEVLLGTDSHTCNAGAFGQFATGIGNTDAGFVMGTGKALLK 237
                   V    +G   P  V+ GTDSHT    + G    G+G  +A   M      + 
Sbjct: 127 VNLEYLARVVFNADGIMYPDSVV-GTDSHTTMINSLGVAGWGVGGIEAIVAMLGQPMDMV 185

Query: 238 VPPTIRFVLDGEMPPYLLAKDLILQIIGEISVSGATYKSMEFVGSTVESLNMEERMTLCN 297
           +P  + F L G +   + A DL+L I   +   G   K +EF G  V  L++  R T+ N
Sbjct: 186 LPGVVGFKLSGMLRDGVTATDLVLTITQMLRKHGVVGKFVEFYGVGVGELSLPARATIAN 245

Query: 298 MVIEAGGKNGVVPADQTTFNYLE--GKT--SVEYEPVYSDAQARFVSDY----------- 342
           M  E G   G  P D  T +YL+  G++  +V     Y  A   FV  +           
Sbjct: 246 MSPEYGASMGFFPVDHVTLDYLKLTGRSNETVSMIEAYLRANNMFVEHHEPHTERVYSSY 305

Query: 343 -RFDVSKLEPVIAKPHSPDNRALARECK 369
              ++  +EP I+ P  P +R   +E K
Sbjct: 306 LELNLIDVEPCISGPKRPHDRVPLKEMK 333



>Os03g0136900 Similar to Aconitate hydratase, cytoplasmic (EC 4.2.1.3) (Citrate
           hydro-lyase) (Aconitase)
          Length = 986

 Score = 72.0 bits (175), Expect = 1e-12,   Method: Compositional matrix adjust.
 Identities = 102/455 (22%), Positives = 161/455 (35%), Gaps = 85/455 (18%)

Query: 131 VVIIPDHYIFTSDERANRNVDILRDFCMEQNIKYFYDIK----DLSNFKANPDYKGVCH- 185
           V ++ DH +     R+   V    +    +N + F  +K       N    P   G+ H 
Sbjct: 212 VDLVIDHSVQVDVARSENAVQANMELEFHRNKERFGFLKWGSTAFRNMLVVPPGSGIVHQ 271

Query: 186 --------VALAQEGHCRPGEVLLGTDSHTCNAGAFGQFATGIGNTDAGFVMGTGKALLK 237
                   V     G   P  V+ GTDSHT      G    G+G  +A   M      + 
Sbjct: 272 VNLEYLARVVFNNGGILYPDSVV-GTDSHTTMIDGLGVAGWGVGGIEAEATMLGQPMSMV 330

Query: 238 VPPTIRFVLDGEMPPYLLAKDLILQIIGEISVSGATYKSMEFVGSTVESLNMEERMTLCN 297
           +P  + F L G++   + A DL+L +   +   G   K +EF G  +  L++ +R T+ N
Sbjct: 331 LPGVVGFKLTGKLRNGVTATDLVLTVTQMLRKHGVVGKFVEFYGGGMSELSLADRATIAN 390

Query: 298 MVIEAGGKNGVVPADQTTFNYLE------------------GKTSVEYEPVYSDAQARFV 339
           M  E G   G  P D  T +YL+                   K  V+Y     +A+  + 
Sbjct: 391 MSPEYGATMGFFPVDGKTLDYLKLTGRSDDTVAMIESYLRANKMFVDYN--QPEAERVYS 448

Query: 340 SDYRFDVSKLEPVIAKPHSPDNRALARECK---------DVKIDRVYIGSCTGGKTEDF- 389
           S    ++ ++EP ++ P  P +R   +  K         DV      +   + GK  +F 
Sbjct: 449 SYLELNLEEVEPCLSGPKRPHDRVTLKNMKSDWLSCLDNDVGFKGFAVPKESQGKVAEFS 508

Query: 390 FAAAKVFLASGKKVKV----------PTFLVPATQ------------KVWMDIYSIPVPG 427
           F      L  G  V            P  ++ A              K W  I +   PG
Sbjct: 509 FHGTPAKLKHGDVVIAAITSCTNTSNPNVMLGAALVAKKACELGLEVKPW--IKTSLAPG 566

Query: 428 AGGKTCSQIFEEAGCDTPASP--------SCGACLGGPRDTYARMNEPM-------VCVS 472
           +G     +  +++G                C  C+G   +    ++  +         V 
Sbjct: 567 SG--VVKKYLDKSGLQKYLDQLGFHIVGYGCTTCIGNSGELDETVSAAISDNDIVAAAVL 624

Query: 473 TTNRNFPGRMGHKEGQIYLASPFTAAASALTGYVT 507
           + NRNF GR+       YLASP    A AL G V 
Sbjct: 625 SGNRNFEGRVHALTRANYLASPPLVVAYALAGTVN 659



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.135    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 17,039,134
Number of extensions: 745947
Number of successful extensions: 1311
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 1310
Number of HSP's successfully gapped: 3
Length of query: 514
Length of database: 17,035,801
Length adjustment: 105
Effective length of query: 409
Effective length of database: 11,553,331
Effective search space: 4725312379
Effective search space used: 4725312379
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 158 (65.5 bits)