BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0931400 Os01g0931400|AK105048
         (264 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0931400  Thiamine pyrophosphokinase family protein           503   e-143
Os05g0367400  Thiamine pyrophosphokinase family protein           397   e-111
Os01g0356500  Thiamine pyrophosphokinase family protein           330   7e-91

>Os01g0931400 Thiamine pyrophosphokinase family protein
          Length = 264

 Score =  503 bits (1295), Expect = e-143,   Method: Compositional matrix adjust.
 Identities = 247/264 (93%), Positives = 247/264 (93%)

Query: 1   MPLPTMTHSSSFLRLXXXXXXXXXXXXXXXXXYAVVVLNQRLPRFAPLLWDRARLRVCAD 60
           MPLPTMTHSSSFLRL                 YAVVVLNQRLPRFAPLLWDRARLRVCAD
Sbjct: 1   MPLPTMTHSSSFLRLPATSSPHPPPADDASAAYAVVVLNQRLPRFAPLLWDRARLRVCAD 60

Query: 61  GGANRVFDGMPELLPAEDPDQVRMRYKPDVIKGDMDSIRPEVKEYYSNLGAEIVDESHDQ 120
           GGANRVFDGMPELLPAEDPDQVRMRYKPDVIKGDMDSIRPEVKEYYSNLGAEIVDESHDQ
Sbjct: 61  GGANRVFDGMPELLPAEDPDQVRMRYKPDVIKGDMDSIRPEVKEYYSNLGAEIVDESHDQ 120

Query: 121 DTTDLHKCVSFITRNPPGSEESNLYILVLGALGGRFDHEMGNINVLYRFSNIRIVLLSDD 180
           DTTDLHKCVSFITRNPPGSEESNLYILVLGALGGRFDHEMGNINVLYRFSNIRIVLLSDD
Sbjct: 121 DTTDLHKCVSFITRNPPGSEESNLYILVLGALGGRFDHEMGNINVLYRFSNIRIVLLSDD 180

Query: 181 CSIFLLPKTHSHEIHIERSIEGPHCGLIPMGSPSASTTTTGLRWNLDNTSMSYGGLISTS 240
           CSIFLLPKTHSHEIHIERSIEGPHCGLIPMGSPSASTTTTGLRWNLDNTSMSYGGLISTS
Sbjct: 181 CSIFLLPKTHSHEIHIERSIEGPHCGLIPMGSPSASTTTTGLRWNLDNTSMSYGGLISTS 240

Query: 241 NIVEEETVRITSDSDLIWTISLRN 264
           NIVEEETVRITSDSDLIWTISLRN
Sbjct: 241 NIVEEETVRITSDSDLIWTISLRN 264



>Os05g0367400 Thiamine pyrophosphokinase family protein
          Length = 267

 Score =  397 bits (1021), Expect = e-111,   Method: Compositional matrix adjust.
 Identities = 196/265 (73%), Positives = 221/265 (83%), Gaps = 4/265 (1%)

Query: 2   PLPTMTHSSSFLRLXXXXXXXXXXXXXXXXXYAVVVLNQRLPRFAPLLWDRARLRVCADG 61
           P P M+HSS+FL                   YA++VLNQRLPRFAP LWDRA++RVCADG
Sbjct: 3   PRPAMSHSSAFL--LPSPSAAAAGADADGAAYALLVLNQRLPRFAPRLWDRAQVRVCADG 60

Query: 62  GANRVFDGMPELLPAEDPDQVRMRYKPDVIKGDMDSIRPEVKEYYSNLGAEIVDESHDQD 121
           GANRVFDGMPEL P +DPD+VR RYKPDVIKGD+DS+RPEVKEYYSN+G +IVDESHDQD
Sbjct: 61  GANRVFDGMPELFPGQDPDEVRRRYKPDVIKGDLDSVRPEVKEYYSNMGTQIVDESHDQD 120

Query: 122 TTDLHKCVSFITRNPPGSEESNLYILVLGALGGRFDHEMGNINVLYRFSNIRIVLLSDDC 181
           TTDLHKCV+FIT N     +SNL I  LGALGGRFDHEMGNINVL+ F N RI+LLSDDC
Sbjct: 121 TTDLHKCVAFITENSAIPNKSNLCIFALGALGGRFDHEMGNINVLHLFPNNRIILLSDDC 180

Query: 182 SIFLLPKTHSHEIHIERSIEGPHCGLIPMGSPSASTTTTGLRWNLDNTSMSYGGLISTSN 241
            IFLLP+TH+H IHIERSIEGPHCGLIP+G+PSA+TTTTGL+WNLDNTSMS+GGLISTSN
Sbjct: 181 LIFLLPRTHTHNIHIERSIEGPHCGLIPIGAPSATTTTTGLQWNLDNTSMSFGGLISTSN 240

Query: 242 IVEEET--VRITSDSDLIWTISLRN 264
           IV EE+  V ITSDSDLIWTISLR+
Sbjct: 241 IVREESTVVTITSDSDLIWTISLRH 265



>Os01g0356500 Thiamine pyrophosphokinase family protein
          Length = 267

 Score =  330 bits (846), Expect = 7e-91,   Method: Compositional matrix adjust.
 Identities = 157/262 (59%), Positives = 194/262 (74%), Gaps = 5/262 (1%)

Query: 3   LPTMTHSSSFLRLXXXXXXXXXXXXXXXXXYAVVVLNQRLPRFAPLLWDRARLRVCADGG 62
           +  M HSS+FL                   YA+VVLNQ LPRF P LW  A+LR+CADGG
Sbjct: 9   MEVMVHSSTFL-----LPKLHQPANSPAKKYALVVLNQNLPRFVPRLWTHAKLRICADGG 63

Query: 63  ANRVFDGMPELLPAEDPDQVRMRYKPDVIKGDMDSIRPEVKEYYSNLGAEIVDESHDQDT 122
           ANR+FD M ++    D +  R RY P++I+GDMDSIRPEVK++YS+ G++I D+SH+Q+T
Sbjct: 64  ANRIFDEMFQMTNDPDYESTRKRYIPEIIEGDMDSIRPEVKQFYSSQGSKISDKSHNQET 123

Query: 123 TDLHKCVSFITRNPPGSEESNLYILVLGALGGRFDHEMGNINVLYRFSNIRIVLLSDDCS 182
           TDLHKC+S I R  P  E++NL +LV GALGGRFDHE  NIN+LY FS++RIVLLSDDC 
Sbjct: 124 TDLHKCISRIHRCTPDHEKTNLCVLVTGALGGRFDHEAANINILYLFSDMRIVLLSDDCL 183

Query: 183 IFLLPKTHSHEIHIERSIEGPHCGLIPMGSPSASTTTTGLRWNLDNTSMSYGGLISTSNI 242
           I LLPKTH HEI+IE S+EGPHCGL P+G+PS STTTTGL+WNL    M +G +ISTSNI
Sbjct: 184 IRLLPKTHKHEIYIESSVEGPHCGLFPVGAPSGSTTTTGLKWNLSEAKMRFGSMISTSNI 243

Query: 243 VEEETVRITSDSDLIWTISLRN 264
           V  E V + SD+DL+WTISLRN
Sbjct: 244 VHAEKVTVQSDADLLWTISLRN 265



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.137    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 9,330,405
Number of extensions: 403754
Number of successful extensions: 853
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 850
Number of HSP's successfully gapped: 3
Length of query: 264
Length of database: 17,035,801
Length adjustment: 99
Effective length of query: 165
Effective length of database: 11,866,615
Effective search space: 1957991475
Effective search space used: 1957991475
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 155 (64.3 bits)