BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os01g0918300 Os01g0918300|AK058869
(100 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os01g0918300 Ubiquitin-like protein SMT3 206 2e-54
Os01g0918200 Similar to Ubiquitin-like protein SMT3 187 2e-48
Os07g0574300 77 2e-15
Os07g0574500 Ubiquitin domain containing protein 74 3e-14
>Os01g0918300 Ubiquitin-like protein SMT3
Length = 100
Score = 206 bits (525), Expect = 2e-54, Method: Compositional matrix adjust.
Identities = 100/100 (100%), Positives = 100/100 (100%)
Query: 1 MSAAGEEDKKPAGGEGGGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQSVDMNA 60
MSAAGEEDKKPAGGEGGGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQSVDMNA
Sbjct: 1 MSAAGEEDKKPAGGEGGGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQSVDMNA 60
Query: 61 IAFLFDGRRLRGEQTPDELEMEDGDEIDAMLHQTGGCLPA 100
IAFLFDGRRLRGEQTPDELEMEDGDEIDAMLHQTGGCLPA
Sbjct: 61 IAFLFDGRRLRGEQTPDELEMEDGDEIDAMLHQTGGCLPA 100
>Os01g0918200 Similar to Ubiquitin-like protein SMT3
Length = 101
Score = 187 bits (474), Expect = 2e-48, Method: Compositional matrix adjust.
Identities = 89/99 (89%), Positives = 94/99 (94%)
Query: 2 SAAGEEDKKPAGGEGGGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQSVDMNAI 61
S AGE++KKPAGGEGGGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQSVD+ +I
Sbjct: 3 SPAGEDEKKPAGGEGGGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQSVDIKSI 62
Query: 62 AFLFDGRRLRGEQTPDELEMEDGDEIDAMLHQTGGCLPA 100
AFLFDGRRL EQTPD+LEMEDGDEIDAMLHQTGG LPA
Sbjct: 63 AFLFDGRRLNAEQTPDQLEMEDGDEIDAMLHQTGGSLPA 101
>Os07g0574300
Length = 130
Score = 77.4 bits (189), Expect = 2e-15, Method: Compositional matrix adjust.
Identities = 44/98 (44%), Positives = 57/98 (58%), Gaps = 3/98 (3%)
Query: 2 SAAGEEDKKPAGGEGGGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQS--VDMN 59
+ A E K PA G ++ LKV+G DG V+ + R+ +L+ LM+ Y DR V
Sbjct: 21 TPATWEWKAPAT-RVAGEYVTLKVQGTDGRAVYRTMLRTEELQGLMDFYYDRSHGRVQRG 79
Query: 60 AIAFLFDGRRLRGEQTPDELEMEDGDEIDAMLHQTGGC 97
FLFDGRRLRG QTP EL+MEDGDE++ GG
Sbjct: 80 TGRFLFDGRRLRGWQTPAELQMEDGDEVNFFEELIGGA 117
>Os07g0574500 Ubiquitin domain containing protein
Length = 110
Score = 73.6 bits (179), Expect = 3e-14, Method: Compositional matrix adjust.
Identities = 40/94 (42%), Positives = 54/94 (57%), Gaps = 2/94 (2%)
Query: 6 EEDKKPAGGEGGGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQS--VDMNAIAF 63
E+D K + G ++ LKV+ DG V+ ++ + QL+ LM+ Y DR V F
Sbjct: 16 EDDGKTPAAKRAGEYVTLKVQDTDGRAVYRTMRWTEQLQGLMDFYYDRAHGRVQRGTGRF 75
Query: 64 LFDGRRLRGEQTPDELEMEDGDEIDAMLHQTGGC 97
L+DGRRL G QTP EL+MEDGDE+D GG
Sbjct: 76 LYDGRRLSGWQTPAELDMEDGDEVDFFEELIGGA 109
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.316 0.136 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,210,491
Number of extensions: 172095
Number of successful extensions: 587
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 590
Number of HSP's successfully gapped: 4
Length of query: 100
Length of database: 17,035,801
Length adjustment: 69
Effective length of query: 31
Effective length of database: 13,433,035
Effective search space: 416424085
Effective search space used: 416424085
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 149 (62.0 bits)