BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0853800 Os01g0853800|AK069147
         (327 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0853800  C2 calcium/lipid-binding region, CaLB domain c...   276   1e-74
Os09g0571200  C2 domain containing protein                         88   9e-18
Os08g0562600  C2 calcium/lipid-binding region, CaLB domain c...    84   1e-16

>Os01g0853800 C2 calcium/lipid-binding region, CaLB domain containing protein
          Length = 327

 Score =  276 bits (707), Expect = 1e-74,   Method: Compositional matrix adjust.
 Identities = 162/282 (57%), Positives = 162/282 (57%)

Query: 46  KAIDLEVTVVSGKHLKNVNWRRGDLRAYVVAYLDPSRRAATRPDDVGGCKPAWNERVVXX 105
           KAIDLEVTVVSGKHLKNVNWRRGDLRAYVVAYLDPSRRAATRPDDVGGCKPAWNERVV  
Sbjct: 46  KAIDLEVTVVSGKHLKNVNWRRGDLRAYVVAYLDPSRRAATRPDDVGGCKPAWNERVVLP 105

Query: 106 XXXXXXXXXXXXXXXXXVFHSKPSDSPKPLVGSARSPLRDLLFXXXXXXXXXXXXXXLIT 165
                            VFHSKPSDSPKPLVGSARSPLRDLLF              LIT
Sbjct: 106 LPPHLSPHDPSLLLSLDVFHSKPSDSPKPLVGSARSPLRDLLFSTNPNPSPDSPASALIT 165

Query: 166 LPLLRPSGRPQGKLRIRLALRERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGS 225
           LPLLRPSGRPQGKLRIRLALRER                                   GS
Sbjct: 166 LPLLRPSGRPQGKLRIRLALRERSPPPPEPQYPPPSSSPYYFPPPPPPAYSAPPPPQYGS 225

Query: 226 EQXXXXXXXXXXXXXXXXXXXXXXXSAPVEYNRQYDPRAXXXXXXXXXXXXXXXXXXXXX 285
           EQ                       SAPVEYNRQYDPRA                     
Sbjct: 226 EQYYRSGGYYSAPPPPPQYEYTAGPSAPVEYNRQYDPRAGSGSGNGRYGVGTGIAVGTVT 285

Query: 286 XXXXXXXXDEGVKYKEEKSADRVEDKVVPAGRDDDSRGYREY 327
                   DEGVKYKEEKSADRVEDKVVPAGRDDDSRGYREY
Sbjct: 286 GALGGLAIDEGVKYKEEKSADRVEDKVVPAGRDDDSRGYREY 327



>Os09g0571200 C2 domain containing protein
          Length = 290

 Score = 87.8 bits (216), Expect = 9e-18,   Method: Compositional matrix adjust.
 Identities = 53/139 (38%), Positives = 73/139 (52%), Gaps = 8/139 (5%)

Query: 49  DLEVTVVSGKHLKNVNWRRGDLRAYVVAYLDPSRRAATRPDDVGGCKPAWNERVVXXXXX 108
           ++EVTV S + LKNVNWR GDL+ Y V ++D   + +TR D      P W++++      
Sbjct: 7   EVEVTVASARDLKNVNWRNGDLKPYAVVWIDDGAKCSTRVDLDNADNPTWDDKLT--VPL 64

Query: 109 XXXXXXXXXXXXXXVFHSKPSDSPKPLVGSARSPLRDLLFXXXXXXXXXXXXXXLITLPL 168
                         V H+  +D  KPLVGSAR PLRD+L                 +L L
Sbjct: 65  PPSTRLDDAVLYLDVVHANATDGVKPLVGSARLPLRDVLADTGIGARASR------SLRL 118

Query: 169 LRPSGRPQGKLRIRLALRE 187
            RPSGRP G+L +R+A+RE
Sbjct: 119 KRPSGRPHGRLEVRVAVRE 137



>Os08g0562600 C2 calcium/lipid-binding region, CaLB domain containing protein
          Length = 291

 Score = 84.0 bits (206), Expect = 1e-16,   Method: Compositional matrix adjust.
 Identities = 54/139 (38%), Positives = 74/139 (53%), Gaps = 8/139 (5%)

Query: 49  DLEVTVVSGKHLKNVNWRRGDLRAYVVAYLDPSRRAATRPDDVGGCKPAWNERVVXXXXX 108
           ++EVTV S + LKNVNWR GDL+ Y V ++D   + +TR D      P W++++      
Sbjct: 6   EVEVTVSSARDLKNVNWRNGDLKPYAVLWVDDGAKCSTRVDLDNADNPNWDDKLT--LPL 63

Query: 109 XXXXXXXXXXXXXXVFHSKPSDSPKPLVGSARSPLRDLLFXXXXXXXXXXXXXXLITLPL 168
                         V H+  ++  KPLVGSAR PLRD+L                 +L L
Sbjct: 64  PPSSRLDDALLYLDVVHANAAEGVKPLVGSARLPLRDVLDDAGVGARVSR------SLRL 117

Query: 169 LRPSGRPQGKLRIRLALRE 187
            RPSGRPQG+L +RLA+RE
Sbjct: 118 KRPSGRPQGRLDLRLAVRE 136



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.316    0.136    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,218,328
Number of extensions: 173993
Number of successful extensions: 274
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 267
Number of HSP's successfully gapped: 3
Length of query: 327
Length of database: 17,035,801
Length adjustment: 101
Effective length of query: 226
Effective length of database: 11,762,187
Effective search space: 2658254262
Effective search space used: 2658254262
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 156 (64.7 bits)