BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0840200 Os01g0840200|AK069954
         (521 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0840200  HSP20-like chaperone domain containing protein     1030   0.0  
Os01g0151100  HSP20-like chaperone domain containing protein      617   e-177
Os03g0277100  HSP20-like chaperone domain containing protein      255   7e-68

>Os01g0840200 HSP20-like chaperone domain containing protein
          Length = 521

 Score = 1030 bits (2663), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 496/521 (95%), Positives = 496/521 (95%)

Query: 1   MGEDLVTTLSMENXXXXXXXXHHSLCTLLSMDPAGHLDDRAVGVMVQPRIGDGAGRAHAV 60
           MGEDLVTTLSMEN        HHSLCTLLSMDPAGHLDDRAVGVMVQPRIGDGAGRAHAV
Sbjct: 1   MGEDLVTTLSMENGGGGGGGGHHSLCTLLSMDPAGHLDDRAVGVMVQPRIGDGAGRAHAV 60

Query: 61  SLSGAHPPDINQPWQTDLCDMLDVSLGPQIQVYDAEALAVLSSAPKAGNRKAAKRGDSIW 120
           SLSGAHPPDINQPWQTDLCDMLDVSLGPQIQVYDAEALAVLSSAPKAGNRKAAKRGDSIW
Sbjct: 61  SLSGAHPPDINQPWQTDLCDMLDVSLGPQIQVYDAEALAVLSSAPKAGNRKAAKRGDSIW 120

Query: 121 GAWFFFTFYFKPLLADKCKGKVTRDANGVSGFDKTDLRLDMFMVQHDMENMYMWVFKERP 180
           GAWFFFTFYFKPLLADKCKGKVTRDANGVSGFDKTDLRLDMFMVQHDMENMYMWVFKERP
Sbjct: 121 GAWFFFTFYFKPLLADKCKGKVTRDANGVSGFDKTDLRLDMFMVQHDMENMYMWVFKERP 180

Query: 181 ENALGKMQLRSYMNGHSRPGEPQFPFSVDRGFVRSHRMQRKHYRGLSNPQCIHGIEVVRS 240
           ENALGKMQLRSYMNGHSRPGEPQFPFSVDRGFVRSHRMQRKHYRGLSNPQCIHGIEVVRS
Sbjct: 181 ENALGKMQLRSYMNGHSRPGEPQFPFSVDRGFVRSHRMQRKHYRGLSNPQCIHGIEVVRS 240

Query: 241 PNLASITEVDRRRWVELTGRELNFAIPQEACDFGTWRTMPNTEIELDRPHPVMKGNVHQH 300
           PNLASITEVDRRRWVELTGRELNFAIPQEACDFGTWRTMPNTEIELDRPHPVMKGNVHQH
Sbjct: 241 PNLASITEVDRRRWVELTGRELNFAIPQEACDFGTWRTMPNTEIELDRPHPVMKGNVHQH 300

Query: 301 PKKXXXXXXXXXXXXXXXXXEDGMDLSPVSSKRRKEVFPHAMDEECFLPLNSCGERTQQD 360
           PKK                 EDGMDLSPVSSKRRKEVFPHAMDEECFLPLNSCGERTQQD
Sbjct: 301 PKKLLNGSGLNLSSPSNHSGEDGMDLSPVSSKRRKEVFPHAMDEECFLPLNSCGERTQQD 360

Query: 361 VEMHSVVQPSWQHEFSGVMKKARGPVTAAKSIYEDDQGYLIMVSLPFVDQQKVKVSWRNS 420
           VEMHSVVQPSWQHEFSGVMKKARGPVTAAKSIYEDDQGYLIMVSLPFVDQQKVKVSWRNS
Sbjct: 361 VEMHSVVQPSWQHEFSGVMKKARGPVTAAKSIYEDDQGYLIMVSLPFVDQQKVKVSWRNS 420

Query: 421 LTHGIVKILCVSTAQTPYIRRHDRVFKLADPMPEHCPHGEFVREIPLATRIPEDAKLEAY 480
           LTHGIVKILCVSTAQTPYIRRHDRVFKLADPMPEHCPHGEFVREIPLATRIPEDAKLEAY
Sbjct: 421 LTHGIVKILCVSTAQTPYIRRHDRVFKLADPMPEHCPHGEFVREIPLATRIPEDAKLEAY 480

Query: 481 FDEAAAVLEIMVPKRGNEPEEHEVRVSMRPPHLAANDLLLT 521
           FDEAAAVLEIMVPKRGNEPEEHEVRVSMRPPHLAANDLLLT
Sbjct: 481 FDEAAAVLEIMVPKRGNEPEEHEVRVSMRPPHLAANDLLLT 521



>Os01g0151100 HSP20-like chaperone domain containing protein
          Length = 547

 Score =  617 bits (1591), Expect = e-177,   Method: Compositional matrix adjust.
 Identities = 321/558 (57%), Positives = 374/558 (67%), Gaps = 48/558 (8%)

Query: 1   MGEDLVTTLSMENXXXXXXXXHHSLCTLLSMDPAGHLDDRAVGVMVQPRIGDGAGRAHAV 60
           MGE L+TTLSMEN            CT LSMDPAG               G         
Sbjct: 1   MGEPLLTTLSMENTNSHP-------CTRLSMDPAGSHAASGDSSGGGGGGGSTGAGGGGG 53

Query: 61  SL------------SGAHP--PDINQP---------------WQTDLCDMLDVSLGPQIQ 91
                           AHP  PDIN P               W  D  DMLDV LG   Q
Sbjct: 54  GGGGDRELFIIPRRESAHPGPPDINLPLSADPSPPPPPHPPSWGIDQFDMLDVGLG--TQ 111

Query: 92  VYDAEALAVL-------SSAPKAGNRKAAKRGDSIWGAWFFFTFYFKPLLADKCKGKVTR 144
            Y++E    L       ++A   G RK AKRGDSIWGAWFFF  YFKP L +K KGKVTR
Sbjct: 112 TYESEVALTLPKLTGNGNTAVGVGARKCAKRGDSIWGAWFFFNHYFKPALVEKPKGKVTR 171

Query: 145 DANG-VSGFDKTDLRLDMFMVQHDMENMYMWVFKERPENALGKMQLRSYMNGHSRPGEPQ 203
           D++G VSGF+K+DLRLD+F+VQHDMENMYMWVFKERP+NALGKMQLRS+MNGHS+ GEP 
Sbjct: 172 DSSGSVSGFEKSDLRLDVFLVQHDMENMYMWVFKERPDNALGKMQLRSFMNGHSKHGEPS 231

Query: 204 FPFSVDRGFVRSHRMQRKHYRGLSNPQCIHGIEVVRSPNLASITEVDRRRWVELTGRELN 263
           FPFS D+GF RSHRMQRKHYRGLSNPQC+HGIE+V SPNL+++ E + +RW ELTGRELN
Sbjct: 232 FPFSADKGFARSHRMQRKHYRGLSNPQCLHGIEIVSSPNLSAVPEAEMKRWAELTGRELN 291

Query: 264 FAIPQEACDFGTWRTMPNTEIELDRPHPVMKGNVHQHPKKXXXXXXXXXXXXXXXXXEDG 323
           F+IP EA DF +WR +P+T+ ELDRP P+     H                      +DG
Sbjct: 292 FSIPPEASDFESWRNLPSTDFELDRPLPLSSKITHGSHSHKKALNGSGLNLSTPPSSDDG 351

Query: 324 MDLSPVSSKRRKEVFPHAMDEECFLPLNSCGERTQQDVEMHSVVQPSWQHEFSGVMKKAR 383
           MDLSP  +KRRK+ F H  DE+C +  NSC +R +Q++E+H+  +PSW HEF+GV K A 
Sbjct: 352 MDLSPKCAKRRKDFFAHGADEDCVMANNSCSDR-EQEIEVHT-GEPSWMHEFTGVAKHAS 409

Query: 384 GPVTAAKSIYEDDQGYLIMVSLPFVDQQKVKVSWRNSLTHGIVKILCVSTAQTPYIRRHD 443
           GPVTAAK+IYEDD+GYLIMVS+ F D   VKVSWRN+LTHGIVKI CVSTA+ P+++RHD
Sbjct: 410 GPVTAAKTIYEDDEGYLIMVSMLFSDPHSVKVSWRNTLTHGIVKISCVSTARMPFVKRHD 469

Query: 444 RVFKLADPMPEHCPHGEFVREIPLATRIPEDAKLEAYFDEAAAVLEIMVPKRGNEPEEHE 503
           R FKL DP PEHCP GEFVREIPLATRIPEDAKLEAY+DE    LEIMVPK    PEEHE
Sbjct: 470 RTFKLTDPFPEHCPPGEFVREIPLATRIPEDAKLEAYYDETGTGLEIMVPKHRVGPEEHE 529

Query: 504 VRVSMRPPHLAANDLLLT 521
           V+V MRPPHL  NDLLL+
Sbjct: 530 VQVCMRPPHLGENDLLLS 547



>Os03g0277100 HSP20-like chaperone domain containing protein
          Length = 242

 Score =  255 bits (651), Expect = 7e-68,   Method: Compositional matrix adjust.
 Identities = 127/240 (52%), Positives = 165/240 (68%), Gaps = 7/240 (2%)

Query: 275 TWRTMPNTEIELDRPHPV--MKGNVHQHPKKXXXXXXXXXXXXXXXXXE-DGMDLSPVSS 331
           +WR +P+T+ EL+RP      K + H H KK                   DG+D+  + +
Sbjct: 1   SWRNLPSTDFELERPATTAATKTSSHGHHKKLLNGSGLNLSTQPSNHSSGDGLDIPNICN 60

Query: 332 KRRKEVFPHAMDEECFLPLNSCGERTQQDVEMHSVVQPSWQHEFSGVMKKARGPVTAAKS 391
           KRRK+  P AM+E+C    NS  ++ Q D+E+    +PSW ++F+GVM+ A GPVTAAK+
Sbjct: 61  KRRKDSSPTAMEEDCS---NSNSDKVQ-DMEVSHTFEPSWMNDFTGVMRHASGPVTAAKT 116

Query: 392 IYEDDQGYLIMVSLPFVDQQKVKVSWRNSLTHGIVKILCVSTAQTPYIRRHDRVFKLADP 451
           IYED +GYLI++SLPF D Q+VKVSW+N+LT+GIVK+ C S  + P+++RHDR FKL DP
Sbjct: 117 IYEDSKGYLIIISLPFADIQRVKVSWKNTLTNGIVKVSCTSVGRMPFLKRHDRTFKLVDP 176

Query: 452 MPEHCPHGEFVREIPLATRIPEDAKLEAYFDEAAAVLEIMVPKRGNEPEEHEVRVSMRPP 511
            PEHCP GEF+REIPL TRIPEDA LEAY DE+   LEI+VPK    PEEHEV VSMRPP
Sbjct: 177 TPEHCPPGEFIREIPLPTRIPEDATLEAYCDESGTGLEIIVPKYRVGPEEHEVHVSMRPP 236



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.135    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 18,593,019
Number of extensions: 783684
Number of successful extensions: 1418
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 1409
Number of HSP's successfully gapped: 3
Length of query: 521
Length of database: 17,035,801
Length adjustment: 105
Effective length of query: 416
Effective length of database: 11,553,331
Effective search space: 4806185696
Effective search space used: 4806185696
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 158 (65.5 bits)