BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0808900 Os01g0808900|Os01g0808900
         (184 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0808900  Similar to SigA binding protein                     145   2e-35
Os05g0390800  VQ domain containing protein                         99   1e-21
Os05g0491700  VQ domain containing protein                         68   5e-12

>Os01g0808900 Similar to SigA binding protein
          Length = 184

 Score =  145 bits (365), Expect = 2e-35,   Method: Compositional matrix adjust.
 Identities = 87/184 (47%), Positives = 87/184 (47%)

Query: 1   MDRQRQQSSRGNATATRXXXXXXXXXXXXXXXXXXXXPIKVVYISNPMRVKTSAAGFRAL 60
           MDRQRQQSSRGNATATR                    PIKVVYISNPMRVKTSAAGFRAL
Sbjct: 1   MDRQRQQSSRGNATATRGGGSSGKGGGGGVGKAAGKKPIKVVYISNPMRVKTSAAGFRAL 60

Query: 61  VQELTGRNADPSKYSPRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120
           VQELTGRNADPSKYSPR                                           
Sbjct: 61  VQELTGRNADPSKYSPRASADDDDGGGGGGGGELAAASDGAGEPGPGAAAASPDTGAAAA 120

Query: 121 XXXXXXXXXXXXXXXXTFXXXXXXXXXXXXXXXXXXIFRSQLLDTSYSVFSPPTLLYDHP 180
                           TF                  IFRSQLLDTSYSVFSPPTLLYDHP
Sbjct: 121 SDAADALVAAGHPAAATFDDEGGGGGGGYYDDDDDDIFRSQLLDTSYSVFSPPTLLYDHP 180

Query: 181 HSKV 184
           HSKV
Sbjct: 181 HSKV 184



>Os05g0390800 VQ domain containing protein
          Length = 169

 Score = 99.4 bits (246), Expect = 1e-21,   Method: Compositional matrix adjust.
 Identities = 54/81 (66%), Positives = 56/81 (69%), Gaps = 4/81 (4%)

Query: 1  MDRQRQQSSRGNATAT----RXXXXXXXXXXXXXXXXXXXXPIKVVYISNPMRVKTSAAG 56
          MDRQRQQSSRGNA+AT                         PIKVVYISNPMRV+TSAAG
Sbjct: 1  MDRQRQQSSRGNASATATRGGGGGGGGKGGGNGKAAAAGKKPIKVVYISNPMRVRTSAAG 60

Query: 57 FRALVQELTGRNADPSKYSPR 77
          FRALVQELTGRNADPSKYSPR
Sbjct: 61 FRALVQELTGRNADPSKYSPR 81



>Os05g0491700 VQ domain containing protein
          Length = 188

 Score = 67.8 bits (164), Expect = 5e-12,   Method: Compositional matrix adjust.
 Identities = 31/36 (86%), Positives = 33/36 (91%)

Query: 39 IKVVYISNPMRVKTSAAGFRALVQELTGRNADPSKY 74
          IKVVYI+NPMRV TS  GFRALVQELTGR+ADPSKY
Sbjct: 48 IKVVYIANPMRVTTSEEGFRALVQELTGRHADPSKY 83



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.318    0.129    0.370 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,633,997
Number of extensions: 41479
Number of successful extensions: 95
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 93
Number of HSP's successfully gapped: 3
Length of query: 184
Length of database: 17,035,801
Length adjustment: 95
Effective length of query: 89
Effective length of database: 12,075,471
Effective search space: 1074716919
Effective search space used: 1074716919
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 153 (63.5 bits)