BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0769100 Os01g0769100|Os01g0769100
         (370 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0769100  Similar to Lipoic acid synthase-like protein        635   0.0  
Os05g0511500  Similar to Lipoic acid synthase-like protein        550   e-157
Os04g0455800  Similar to Lipoic acid synthetase, mitochondri...   342   3e-94
AK108293                                                          201   5e-52

>Os01g0769100 Similar to Lipoic acid synthase-like protein
          Length = 370

 Score =  635 bits (1637), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 316/370 (85%), Positives = 316/370 (85%)

Query: 1   MMQSSXXXXXXXXXXXXXCGNPVCRSRPGSVSVXXXXXXXXXXXXXXXXXXXXGPYTGRD 60
           MMQSS             CGNPVCRSRPGSVSV                    GPYTGRD
Sbjct: 1   MMQSSLARPLPRPPIRPACGNPVCRSRPGSVSVARCRAEAAPPAPAPAARRAAGPYTGRD 60

Query: 61  PEVKKPAWLRQRAAQGEKYARLRESIGELKLNTVCVEAQCPNIGECWNXXXXXXXXXXXI 120
           PEVKKPAWLRQRAAQGEKYARLRESIGELKLNTVCVEAQCPNIGECWN           I
Sbjct: 61  PEVKKPAWLRQRAAQGEKYARLRESIGELKLNTVCVEAQCPNIGECWNGGGGAGGEGDGI 120

Query: 121 ATATIMVLGDTCTRGCRFCAVKTSNKXXXXXXXXXXNTALAVASWGVDYVVLTSVDRDDL 180
           ATATIMVLGDTCTRGCRFCAVKTSNK          NTALAVASWGVDYVVLTSVDRDDL
Sbjct: 121 ATATIMVLGDTCTRGCRFCAVKTSNKPPPPDPLEPLNTALAVASWGVDYVVLTSVDRDDL 180

Query: 181 PDGGSSHFAQTVRALKELKPGILVECLTSDFRGDLEAVSALANSGLDVFAHNIETVRSLQ 240
           PDGGSSHFAQTVRALKELKPGILVECLTSDFRGDLEAVSALANSGLDVFAHNIETVRSLQ
Sbjct: 181 PDGGSSHFAQTVRALKELKPGILVECLTSDFRGDLEAVSALANSGLDVFAHNIETVRSLQ 240

Query: 241 RIVRDPRAGYDQSLAVLKHAKSCKEGMITKSSIMLGLGETDEEVKQAMIDLRAIGVDILT 300
           RIVRDPRAGYDQSLAVLKHAKSCKEGMITKSSIMLGLGETDEEVKQAMIDLRAIGVDILT
Sbjct: 241 RIVRDPRAGYDQSLAVLKHAKSCKEGMITKSSIMLGLGETDEEVKQAMIDLRAIGVDILT 300

Query: 301 LGQYLQPTERHLTVREYVTPEKFQFWKEYGESVGFRYVASGPLVRSSYRAGELFVQNLVR 360
           LGQYLQPTERHLTVREYVTPEKFQFWKEYGESVGFRYVASGPLVRSSYRAGELFVQNLVR
Sbjct: 301 LGQYLQPTERHLTVREYVTPEKFQFWKEYGESVGFRYVASGPLVRSSYRAGELFVQNLVR 360

Query: 361 NNKPKLPASS 370
           NNKPKLPASS
Sbjct: 361 NNKPKLPASS 370



>Os05g0511500 Similar to Lipoic acid synthase-like protein
          Length = 384

 Score =  550 bits (1417), Expect = e-157,   Method: Compositional matrix adjust.
 Identities = 271/342 (79%), Positives = 286/342 (83%)

Query: 25  RSRPGSVSVXXXXXXXXXXXXXXXXXXXXGPYTGRDPEVKKPAWLRQRAAQGEKYARLRE 84
           R+RP SVS+                    GPYTGRDP  +KPAWLRQRAAQGEKYARLRE
Sbjct: 38  RARPNSVSIVRCDADSPPEGSAVAGWAPPGPYTGRDPAARKPAWLRQRAAQGEKYARLRE 97

Query: 85  SIGELKLNTVCVEAQCPNIGECWNXXXXXXXXXXXIATATIMVLGDTCTRGCRFCAVKTS 144
           S+GELKLNTVCVEAQCPNIGECW+           IATATIM+LGDTCTRGCRFCAVKTS
Sbjct: 98  SLGELKLNTVCVEAQCPNIGECWDGGGGAGGDGDGIATATIMLLGDTCTRGCRFCAVKTS 157

Query: 145 NKXXXXXXXXXXNTALAVASWGVDYVVLTSVDRDDLPDGGSSHFAQTVRALKELKPGILV 204
           NK           TA+AVASWGVDYVVLTSVDRDDLPDGGS HFAQTV+ALKELKPGILV
Sbjct: 158 NKPPPPDALEPLRTAVAVASWGVDYVVLTSVDRDDLPDGGSGHFAQTVKALKELKPGILV 217

Query: 205 ECLTSDFRGDLEAVSALANSGLDVFAHNIETVRSLQRIVRDPRAGYDQSLAVLKHAKSCK 264
           ECLTSDFRGDLEAVS+LA+SGLDVFAHNIETVRSLQRIVRDPRA YDQSLAVLKHAK+CK
Sbjct: 218 ECLTSDFRGDLEAVSSLASSGLDVFAHNIETVRSLQRIVRDPRAAYDQSLAVLKHAKNCK 277

Query: 265 EGMITKSSIMLGLGETDEEVKQAMIDLRAIGVDILTLGQYLQPTERHLTVREYVTPEKFQ 324
           +GM+TKSSIMLGLGETDEEVKQ M DLRAI VDILTLGQYLQPTERHL VREYVTPEKF 
Sbjct: 278 DGMVTKSSIMLGLGETDEEVKQTMCDLRAIDVDILTLGQYLQPTERHLRVREYVTPEKFD 337

Query: 325 FWKEYGESVGFRYVASGPLVRSSYRAGELFVQNLVRNNKPKL 366
           FWKEYGES+GF YVASGPLVRSSYRAGELFVQNLVR  K +L
Sbjct: 338 FWKEYGESLGFLYVASGPLVRSSYRAGELFVQNLVRRKKAEL 379



>Os04g0455800 Similar to Lipoic acid synthetase, mitochondrial precursor
           (Lip-syn) (Lipoate synthase) (mLIP1)
          Length = 382

 Score =  342 bits (877), Expect = 3e-94,   Method: Compositional matrix adjust.
 Identities = 165/308 (53%), Positives = 214/308 (69%), Gaps = 7/308 (2%)

Query: 59  RDPEVKKPAWLRQRAAQGEKYARLRESIGELKLNTVCVEAQCPNIGECWNXXXXXXXXXX 118
           R   + KP W+++    G KYA ++  + ELKL+TVC EA+CPN+GECW+          
Sbjct: 76  RKKPLPKPKWMKETIPGGAKYAGIKAKLRELKLHTVCEEARCPNLGECWSGGETGT---- 131

Query: 119 XIATATIMVLGDTCTRGCRFCAVKTSNKXXXXXXXXXXNTALAVASWGVDYVVLTSVDRD 178
             ATATIM+LGDTCTRGCRFC VKTS            N A A+ASWG++Y+V+TSVDRD
Sbjct: 132 --ATATIMILGDTCTRGCRFCNVKTSRTPPPPDPDEPSNVAQAIASWGLEYIVITSVDRD 189

Query: 179 DLPDGGSSHFAQTVRALKELKPGILVECLTSDFRGDLEAVSALANSGLDVFAHNIETVRS 238
           DLPD GS HFA+TV+ LK LKP +L+E L  DFRGD   V  +A SGL VFAHNIETV  
Sbjct: 190 DLPDQGSGHFAETVQKLKVLKPEMLIEALVPDFRGDPACVEKVATSGLHVFAHNIETVEE 249

Query: 239 LQRIVRDPRAGYDQSLAVLKHAKS-CKEGMITKSSIMLGLGETDEEVKQAMIDLRAIGVD 297
           LQR VRD RA + QS+ VLK AK     G +TK+SIMLG GET ++V      +RA GVD
Sbjct: 250 LQRNVRDHRANFKQSIDVLKLAKEYAPAGTLTKTSIMLGCGETPDQVISTTEKVRAAGVD 309

Query: 298 ILTLGQYLQPTERHLTVREYVTPEKFQFWKEYGESVGFRYVASGPLVRSSYRAGELFVQN 357
           ++T GQY++P++RH+ V EYVTPE F+ ++  G  +GFRYVASGP+VRSSY+AGE +++ 
Sbjct: 310 VMTFGQYMRPSKRHMPVSEYVTPEAFERYRSLGVDMGFRYVASGPMVRSSYKAGEFYIKA 369

Query: 358 LVRNNKPK 365
           ++  ++ K
Sbjct: 370 MIEADRAK 377



>AK108293 
          Length = 190

 Score =  201 bits (512), Expect = 5e-52,   Method: Compositional matrix adjust.
 Identities = 94/172 (54%), Positives = 122/172 (70%)

Query: 192 VRALKELKPGILVECLTSDFRGDLEAVSALANSGLDVFAHNIETVRSLQRIVRDPRAGYD 251
           +  +K   P ILVE L  DF GD   +  +A SGLDVFAHN+ETV      VRD RA Y 
Sbjct: 1   ISKIKTKSPKILVEALVPDFSGDQACIEQVATSGLDVFAHNVETVERTTPSVRDRRAKYR 60

Query: 252 QSLAVLKHAKSCKEGMITKSSIMLGLGETDEEVKQAMIDLRAIGVDILTLGQYLQPTERH 311
           QSLAVL+HAK+ K  +ITK+SIMLG GE D EV+Q + DLR   VD++T GQY++PT+RH
Sbjct: 61  QSLAVLRHAKTVKPDLITKTSIMLGCGEQDAEVEQTLRDLREAEVDVVTFGQYMRPTKRH 120

Query: 312 LTVREYVTPEKFQFWKEYGESVGFRYVASGPLVRSSYRAGELFVQNLVRNNK 363
           + V EYV P+ F  W++  + +GF YVASGPLVRSSY+AGE F++N+++  K
Sbjct: 121 MKVHEYVKPDMFAHWQKRAQGLGFLYVASGPLVRSSYKAGEFFIENVLKQRK 172



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.318    0.133    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 10,274,866
Number of extensions: 359224
Number of successful extensions: 718
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 715
Number of HSP's successfully gapped: 4
Length of query: 370
Length of database: 17,035,801
Length adjustment: 102
Effective length of query: 268
Effective length of database: 11,709,973
Effective search space: 3138272764
Effective search space used: 3138272764
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 157 (65.1 bits)