BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0719000 Os01g0719000|AK073213
         (262 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0719000  Protein of unknown function DUF581 family protein   461   e-130
Os01g0593200  Protein of unknown function DUF581 family protein   110   1e-24
Os03g0665200  Protein of unknown function DUF581 family protein    76   3e-14
Os10g0422600  Protein of unknown function DUF581 family protein    70   2e-12

>Os01g0719000 Protein of unknown function DUF581 family protein
          Length = 262

 Score =  461 bits (1186), Expect = e-130,   Method: Compositional matrix adjust.
 Identities = 229/262 (87%), Positives = 229/262 (87%)

Query: 1   MEESNDQGLAQKSTAPTGFFRVPGLFVRLSSKGLNAVDPDSAWSPTSPLDFXXXXXXXXX 60
           MEESNDQGLAQKSTAPTGFFRVPGLFVRLSSKGLNAVDPDSAWSPTSPLDF         
Sbjct: 1   MEESNDQGLAQKSTAPTGFFRVPGLFVRLSSKGLNAVDPDSAWSPTSPLDFKNLSSSTGS 60

Query: 61  XXXXXXXXXGVEADQKFRTNPPRVGLGLVDALTADESSSCLGVTSSFLDSIRPFLELGLP 120
                    GVEADQKFRTNPPRVGLGLVDALTADESSSCLGVTSSFLDSIRPFLELGLP
Sbjct: 61  TNLKSSGLLGVEADQKFRTNPPRVGLGLVDALTADESSSCLGVTSSFLDSIRPFLELGLP 120

Query: 121 KAASDAAMQKNGSGSVTLDEIADFALSEEYTCVIEHGPNPRTTHILGDETLEVCKGVPRS 180
           KAASDAAMQKNGSGSVTLDEIADFALSEEYTCVIEHGPNPRTTHILGDETLEVCKGVPRS
Sbjct: 121 KAASDAAMQKNGSGSVTLDEIADFALSEEYTCVIEHGPNPRTTHILGDETLEVCKGVPRS 180

Query: 181 SKKSIFTIEPIGELPSTLACAVSRSCCYCRKRLQQDRDIYMYLGEKAFCSNECRRDYXXX 240
           SKKSIFTIEPIGELPSTLACAVSRSCCYCRKRLQQDRDIYMYLGEKAFCSNECRRDY   
Sbjct: 181 SKKSIFTIEPIGELPSTLACAVSRSCCYCRKRLQQDRDIYMYLGEKAFCSNECRRDYIDE 240

Query: 241 XXXXXXXXXXXXSAGSSALLAW 262
                       SAGSSALLAW
Sbjct: 241 EIEEVEELMMLDSAGSSALLAW 262



>Os01g0593200 Protein of unknown function DUF581 family protein
          Length = 260

 Score =  110 bits (275), Expect = 1e-24,   Method: Compositional matrix adjust.
 Identities = 87/249 (34%), Positives = 121/249 (48%), Gaps = 43/249 (17%)

Query: 3   ESNDQGLAQKSTAPTGFFRVPGLFVRLSSKGLNAVDPDSAWSPTSPLDFXXXXXXXXXXX 62
           +++ + +AQK     GFFRVP L V+LSSK L  ++ D+  SPTSPLD            
Sbjct: 10  QTSSESIAQK----MGFFRVPDLLVKLSSKCL--IELDAVRSPTSPLDLIFFPGLGAKSP 63

Query: 63  XXXXXXXGVEADQKFRTNPPRVGLGLVDALTADESSS------CLGVTSSFLDSIRPFLE 116
                               RVGLGLVD+LT D S+        LG      D++     
Sbjct: 64  RSSFLGD-------------RVGLGLVDSLTDDSSTPLGSRKVLLGSEMRITDNVTSKNS 110

Query: 117 LGLPKAA-----SDAAMQKNGSGS-VTLDEIADFALSEEYTCVIEHGPNPRTTHILGDET 170
              P  A      D +M  +  GS ++LD+I +   SE+YT V+  GPNPRTTH  GD  
Sbjct: 111 FTAPVEAGVVDQKDESMCDDLKGSFMSLDDIVN---SEDYTRVVCRGPNPRTTHFFGDHV 167

Query: 171 LEV--CKGVPRSSKKSIFTIEPIGELPSTLACAVSRSCCYCRKRLQQDRDIYMYLGEKAF 228
           LE    + +P  SK           LP  L   +   C +C ++L++ +DIY+Y G+KAF
Sbjct: 168 LEFEGEQLMPDESKSE-------ESLPPRLEEGMMSFCYFCGEKLEEGKDIYVYQGDKAF 220

Query: 229 CSNECRRDY 237
           CS ECR ++
Sbjct: 221 CSMECRENF 229



>Os03g0665200 Protein of unknown function DUF581 family protein
          Length = 302

 Score = 75.9 bits (185), Expect = 3e-14,   Method: Compositional matrix adjust.
 Identities = 45/122 (36%), Positives = 61/122 (50%), Gaps = 18/122 (14%)

Query: 119 LPKAASDAAMQKNGSGSVTLDEIADFALSEEYTCVIEHGPNPRTTHILGDETLEVCKGVP 178
           LP+  S  + +   +  V+  E+A   +SE+YTCVI  GPNPRTTHI  D  +E C  V 
Sbjct: 179 LPEVVSSPSARVFAAAVVSPGEMA---MSEDYTCVISRGPNPRTTHIFDDCIVESCGDV- 234

Query: 179 RSSKKSIFTIEPIGELPSTLACAVSR------SCCYCRKRLQQDRDIYMYLGEKAFCSNE 232
                    +E +            R      SC  C K+L    DI++Y G+KAFCS+E
Sbjct: 235 --------LVEKVAGGGGDGDGDAVRTNGFLNSCYACNKQLGHGNDIFIYRGDKAFCSSE 286

Query: 233 CR 234
           CR
Sbjct: 287 CR 288



>Os10g0422600 Protein of unknown function DUF581 family protein
          Length = 345

 Score = 69.7 bits (169), Expect = 2e-12,   Method: Compositional matrix adjust.
 Identities = 37/89 (41%), Positives = 47/89 (52%), Gaps = 1/89 (1%)

Query: 146 LSEEYTCVIEHGPNPRTTHILGDETLEVCKGVPRSSKKSIFTIEPIGELPSTLACAVSRS 205
           +SE+YTCVI  G NPRTTHI  +  +E   G        ++     G+          R 
Sbjct: 245 MSEDYTCVIARGANPRTTHIFDNRVVE-SSGDGEYFPPELWPPSAAGKGDDDGDGDFLRY 303

Query: 206 CCYCRKRLQQDRDIYMYLGEKAFCSNECR 234
           C  C K L   +DI+MY GEKAFCS+ECR
Sbjct: 304 CHGCSKDLGLGKDIFMYRGEKAFCSHECR 332



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.316    0.133    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 8,183,654
Number of extensions: 312724
Number of successful extensions: 759
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 756
Number of HSP's successfully gapped: 4
Length of query: 262
Length of database: 17,035,801
Length adjustment: 99
Effective length of query: 163
Effective length of database: 11,866,615
Effective search space: 1934258245
Effective search space used: 1934258245
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 155 (64.3 bits)