BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0691300 Os01g0691300|J075112P14
         (112 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0691300  Plant lipid transfer/seed storage/trypsin-alph...   141   1e-34
Os05g0550600  Similar to Nonspecific lipid-transfer protein ...    70   3e-13
Os05g0550300  Similar to Lipid transfer protein (Fragment)         70   5e-13

>Os01g0691300 Plant lipid transfer/seed storage/trypsin-alpha amylase inhibitor
           domain containing protein
          Length = 112

 Score =  141 bits (355), Expect = 1e-34,   Method: Compositional matrix adjust.
 Identities = 77/112 (68%), Positives = 77/112 (68%)

Query: 1   MTTRPSVFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCDLMQLSPCVSAFSGVG 60
           MTTRPSVF                                   CDLMQLSPCVSAFSGVG
Sbjct: 1   MTTRPSVFSTVVAAIAIAALLSSLLLLQATPAAASARASKKASCDLMQLSPCVSAFSGVG 60

Query: 61  QGSPSSACCSKLKAQGSSCLCLYKDDPKVKRIVSSNRTKRVFTACKVPAPNC 112
           QGSPSSACCSKLKAQGSSCLCLYKDDPKVKRIVSSNRTKRVFTACKVPAPNC
Sbjct: 61  QGSPSSACCSKLKAQGSSCLCLYKDDPKVKRIVSSNRTKRVFTACKVPAPNC 112



>Os05g0550600 Similar to Nonspecific lipid-transfer protein 2 (nsLTP2) (7 kDa
           lipid transfer protein)
          Length = 96

 Score = 70.5 bits (171), Expect = 3e-13,   Method: Compositional matrix adjust.
 Identities = 35/69 (50%), Positives = 43/69 (62%), Gaps = 2/69 (2%)

Query: 44  CDLMQLSPCVSAFSGVGQGSPSSACCSKLKAQGSSCLCLYKDDPKVKRIVSSNRTKRVFT 103
           C   QL+PC  A   VG   P++ACC KLKA  +SC C YK DP +K+ V+S   K+VF 
Sbjct: 30  CTPTQLTPCAPAI--VGNSPPTAACCGKLKAHPASCFCQYKKDPNMKKYVNSPNGKKVFA 87

Query: 104 ACKVPAPNC 112
            CKVP P C
Sbjct: 88  TCKVPLPKC 96



>Os05g0550300 Similar to Lipid transfer protein (Fragment)
          Length = 94

 Score = 69.7 bits (169), Expect = 5e-13,   Method: Compositional matrix adjust.
 Identities = 35/69 (50%), Positives = 46/69 (66%), Gaps = 3/69 (4%)

Query: 44  CDLMQLSPCVSAFSGVGQGSPSSACCSKLKAQGSSCLCLYKDDPKVKRIVSSNRTKRVFT 103
           CD +QLSPC SA   +G  SPS++CCS++K Q   CLC Y  DP ++R V+S   K+V  
Sbjct: 29  CDALQLSPCASAI--IGNASPSASCCSRMKEQ-QPCLCQYARDPNLQRYVNSPNGKKVLA 85

Query: 104 ACKVPAPNC 112
           AC VP P+C
Sbjct: 86  ACHVPVPSC 94



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.130    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,836,738
Number of extensions: 87469
Number of successful extensions: 267
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 272
Number of HSP's successfully gapped: 4
Length of query: 112
Length of database: 17,035,801
Length adjustment: 79
Effective length of query: 33
Effective length of database: 12,910,895
Effective search space: 426059535
Effective search space used: 426059535
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 149 (62.0 bits)