BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0659200 Os01g0659200|AK071200
         (230 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0659200  Similar to Vacuolar ATP synthase subunit E (EC...   428   e-120
Os05g0480700  Similar to Vacuolar ATP synthase subunit E (EC...   303   5e-83
Os01g0222500  Similar to Vacuolar ATP synthase subunit E (EC...   287   5e-78

>Os01g0659200 Similar to Vacuolar ATP synthase subunit E (EC 3.6.3.14) (V-ATPase
           E subunit) (Vacuolar proton pump E subunit)
          Length = 230

 Score =  428 bits (1101), Expect = e-120,   Method: Compositional matrix adjust.
 Identities = 214/230 (93%), Positives = 214/230 (93%)

Query: 1   MNDADVAKQIQQMVRFIRQEAEEKASEISVSAEEEFNIEKLQLVEAEKKKIXXXXXXXXX 60
           MNDADVAKQIQQMVRFIRQEAEEKASEISVSAEEEFNIEKLQLVEAEKKKI         
Sbjct: 1   MNDADVAKQIQQMVRFIRQEAEEKASEISVSAEEEFNIEKLQLVEAEKKKIRQEYERKEK 60

Query: 61  XXXXXXXIEYSMQLNASRIKVLQAQDDLVNSMKEDATKQLLRVSHNHHEYKNLLKELVVQ 120
                  IEYSMQLNASRIKVLQAQDDLVNSMKEDATKQLLRVSHNHHEYKNLLKELVVQ
Sbjct: 61  QVEVRKKIEYSMQLNASRIKVLQAQDDLVNSMKEDATKQLLRVSHNHHEYKNLLKELVVQ 120

Query: 121 GLLRLKEPAVLLRCRKEDHHHVESVLHSAKNEYASKAEVHHPEILVDHDVYLPPSPSSHD 180
           GLLRLKEPAVLLRCRKEDHHHVESVLHSAKNEYASKAEVHHPEILVDHDVYLPPSPSSHD
Sbjct: 121 GLLRLKEPAVLLRCRKEDHHHVESVLHSAKNEYASKAEVHHPEILVDHDVYLPPSPSSHD 180

Query: 181 SHERFCSGGVVLASRDGKIVCENTLDARLEVVFRKKLPEIRKLLFGQVTA 230
           SHERFCSGGVVLASRDGKIVCENTLDARLEVVFRKKLPEIRKLLFGQVTA
Sbjct: 181 SHERFCSGGVVLASRDGKIVCENTLDARLEVVFRKKLPEIRKLLFGQVTA 230



>Os05g0480700 Similar to Vacuolar ATP synthase subunit E (EC 3.6.3.14) (V-ATPase
           E subunit) (Vacuolar proton pump E subunit)
          Length = 231

 Score =  303 bits (777), Expect = 5e-83,   Method: Compositional matrix adjust.
 Identities = 148/227 (65%), Positives = 179/227 (78%)

Query: 1   MNDADVAKQIQQMVRFIRQEAEEKASEISVSAEEEFNIEKLQLVEAEKKKIXXXXXXXXX 60
           MNDADV KQIQQMVRFI QEAEEKASEISV+AEEEFNIEKLQLVE+EK++I         
Sbjct: 1   MNDADVGKQIQQMVRFILQEAEEKASEISVAAEEEFNIEKLQLVESEKRRIRQDYERKAK 60

Query: 61  XXXXXXXIEYSMQLNASRIKVLQAQDDLVNSMKEDATKQLLRVSHNHHEYKNLLKELVVQ 120
                  IEYS QLNA+RIKVL+AQD +V  MKEDA K LLRV+ +   Y+ +LK L+VQ
Sbjct: 61  QVDVGRKIEYSTQLNAARIKVLRAQDGVVGEMKEDAGKSLLRVTKDATAYRKVLKGLIVQ 120

Query: 121 GLLRLKEPAVLLRCRKEDHHHVESVLHSAKNEYASKAEVHHPEILVDHDVYLPPSPSSHD 180
            LLRL+EP+V+LRCR+ D  HVESVL +AK EYA KA+V+ P+IL+D  VYLPP  ++ D
Sbjct: 121 SLLRLREPSVVLRCREADRGHVESVLEAAKKEYAEKAKVNLPKILIDGKVYLPPPKTARD 180

Query: 181 SHERFCSGGVVLASRDGKIVCENTLDARLEVVFRKKLPEIRKLLFGQ 227
           +H  FCSGGVV+AS+DGKIVC+NTLDAR+E+ F++KLPEIRK LF Q
Sbjct: 181 AHGPFCSGGVVIASQDGKIVCDNTLDARVEISFKQKLPEIRKKLFSQ 227



>Os01g0222500 Similar to Vacuolar ATP synthase subunit E (EC 3.6.3.14) (V-ATPase
           E subunit) (Vacuolar proton pump E subunit)
          Length = 231

 Score =  287 bits (734), Expect = 5e-78,   Method: Compositional matrix adjust.
 Identities = 155/230 (67%), Positives = 179/230 (77%)

Query: 1   MNDADVAKQIQQMVRFIRQEAEEKASEISVSAEEEFNIEKLQLVEAEKKKIXXXXXXXXX 60
           MND DVA+Q++QM  FIRQEA EKA+EI  +A EEF IEKLQLVEAEKK+I         
Sbjct: 2   MNDGDVARQLKQMTDFIRQEAVEKAAEIEAAAAEEFQIEKLQLVEAEKKRIRLEFERNEK 61

Query: 61  XXXXXXXIEYSMQLNASRIKVLQAQDDLVNSMKEDATKQLLRVSHNHHEYKNLLKELVVQ 120
                  IEYS QLNASR++VLQAQDDL  SM E A K+LL ++ +HH YKNLL+  +VQ
Sbjct: 62  QGDIKKKIEYSKQLNASRLEVLQAQDDLAMSMLEAAGKELLYITRDHHVYKNLLRIFIVQ 121

Query: 121 GLLRLKEPAVLLRCRKEDHHHVESVLHSAKNEYASKAEVHHPEILVDHDVYLPPSPSSHD 180
            LLRLKEPAV+LRCRKED   VESVL SAKNEYA KA ++ PEI+VD +VYLPP+PS ++
Sbjct: 122 SLLRLKEPAVILRCRKEDRELVESVLESAKNEYADKANIYPPEIMVDRNVYLPPAPSHYE 181

Query: 181 SHERFCSGGVVLASRDGKIVCENTLDARLEVVFRKKLPEIRKLLFGQVTA 230
           +H   CSGGVVLASRDGKIVCENTLDARLEVVFRKKLPEIR+ L GQV A
Sbjct: 182 AHGPSCSGGVVLASRDGKIVCENTLDARLEVVFRKKLPEIRRSLLGQVAA 231



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.316    0.131    0.357 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,441,028
Number of extensions: 240686
Number of successful extensions: 807
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 804
Number of HSP's successfully gapped: 3
Length of query: 230
Length of database: 17,035,801
Length adjustment: 97
Effective length of query: 133
Effective length of database: 11,971,043
Effective search space: 1592148719
Effective search space used: 1592148719
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 154 (63.9 bits)