BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0269000 Os01g0269000|AK068875
         (459 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0269000  Similar to Hydroxymethylglutaryl-CoA lyase          728   0.0  
Os12g0134000  Similar to Hydroxymethylglutaryl-CoA lyase          420   e-118
Os11g0137200  Pyruvate carboxyltransferase domain containing...    99   5e-21

>Os01g0269000 Similar to Hydroxymethylglutaryl-CoA lyase
          Length = 459

 Score =  728 bits (1879), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 369/459 (80%), Positives = 369/459 (80%)

Query: 1   MLASKLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGMPAQERSPGHPSGGDARPGA 60
           MLASKLW                                GMPAQERSPGHPSGGDARPGA
Sbjct: 1   MLASKLWSRLASPSSPASPRALSSSSSAAAAARYLLPSSGMPAQERSPGHPSGGDARPGA 60

Query: 61  GLGLAETXXXXXXXXXXXXXXXXXXXDNPAEENPCTRQIYQRQLMPHRFKLFLGEKRHFT 120
           GLGLAET                   DNPAEENPCTRQIYQRQLMPHRFKLFLGEKRHFT
Sbjct: 61  GLGLAETGCGAAGAPRPLPLPCSSSSDNPAEENPCTRQIYQRQLMPHRFKLFLGEKRHFT 120

Query: 121 YANGASLNPQNYRYFXXXXXXXXXXXXNKIIHDLPRSVKIVEVGPRDGLQNEKNIVPTHV 180
           YANGASLNPQNYRYF            NKIIHDLPRSVKIVEVGPRDGLQNEKNIVPTHV
Sbjct: 121 YANGASLNPQNYRYFSSSSGQQSIGIGNKIIHDLPRSVKIVEVGPRDGLQNEKNIVPTHV 180

Query: 181 KIELIQRLATSGLSVVEATSFVSPKWVPQLADAKDVMDVVRNIEGVSLPVLTPNLXXXXX 240
           KIELIQRLATSGLSVVEATSFVSPKWVPQLADAKDVMDVVRNIEGVSLPVLTPNL     
Sbjct: 181 KIELIQRLATSGLSVVEATSFVSPKWVPQLADAKDVMDVVRNIEGVSLPVLTPNLKGFEA 240

Query: 241 XXXXXXXXXXXXXXXXXXXXXXNINCTIKESLARYKDVALAAKELKIPMRGYVSCVVGCP 300
                                 NINCTIKESLARYKDVALAAKELKIPMRGYVSCVVGCP
Sbjct: 241 AVAAGAKEVAVFASASEAFSKSNINCTIKESLARYKDVALAAKELKIPMRGYVSCVVGCP 300

Query: 301 VEGYVPPSNVAHVAKELYDMGCYEVSLGDTIGVGTPGTVVPMLEAVMFFVPKEKLAVHFH 360
           VEGYVPPSNVAHVAKELYDMGCYEVSLGDTIGVGTPGTVVPMLEAVMFFVPKEKLAVHFH
Sbjct: 301 VEGYVPPSNVAHVAKELYDMGCYEVSLGDTIGVGTPGTVVPMLEAVMFFVPKEKLAVHFH 360

Query: 361 DTYGQSLSNILISLQMGVSVVDSSVAGLGGCPYAKGASGNVATEDVVYMLNGLGISTNVD 420
           DTYGQSLSNILISLQMGVSVVDSSVAGLGGCPYAKGASGNVATEDVVYMLNGLGISTNVD
Sbjct: 361 DTYGQSLSNILISLQMGVSVVDSSVAGLGGCPYAKGASGNVATEDVVYMLNGLGISTNVD 420

Query: 421 LGKVMAAGEFICNHLGRQSGSKAAIALGSKVATANASKL 459
           LGKVMAAGEFICNHLGRQSGSKAAIALGSKVATANASKL
Sbjct: 421 LGKVMAAGEFICNHLGRQSGSKAAIALGSKVATANASKL 459



>Os12g0134000 Similar to Hydroxymethylglutaryl-CoA lyase
          Length = 377

 Score =  420 bits (1080), Expect = e-118,   Method: Compositional matrix adjust.
 Identities = 204/298 (68%), Positives = 237/298 (79%)

Query: 150 IIHDLPRSVKIVEVGPRDGLQNEKNIVPTHVKIELIQRLATSGLSVVEATSFVSPKWVPQ 209
           ++ DLPR VKIVEVGPRDGLQNEKN VPT VKIELI +L  SGLSVVEATSFVSPKWVPQ
Sbjct: 75  VLWDLPRFVKIVEVGPRDGLQNEKNTVPTSVKIELIHKLVASGLSVVEATSFVSPKWVPQ 134

Query: 210 LADAKDVMDVVRNIEGVSLPVLTPNLXXXXXXXXXXXXXXXXXXXXXXXXXXXNINCTIK 269
           LADAKDV++ ++++  V  PVLTPNL                           N+NCTIK
Sbjct: 135 LADAKDVVEGIKHVPDVRFPVLTPNLRGFEAAVAAGAKEVAVFASASESFSKSNLNCTIK 194

Query: 270 ESLARYKDVALAAKELKIPMRGYVSCVVGCPVEGYVPPSNVAHVAKELYDMGCYEVSLGD 329
           ESL RY DV  +AK+  I +RGYVSCVVGCPVEG + PS VA+VAKELYDMGC E+SLGD
Sbjct: 195 ESLVRYHDVVTSAKKHGIRIRGYVSCVVGCPVEGTIHPSKVAYVAKELYDMGCSEISLGD 254

Query: 330 TIGVGTPGTVVPMLEAVMFFVPKEKLAVHFHDTYGQSLSNILISLQMGVSVVDSSVAGLG 389
           TIGVGTPG+V+ MLEAVM FVP +K+AVHFHDTYGQ+L+NIL+SLQ+G+++VDSSV+GLG
Sbjct: 255 TIGVGTPGSVLAMLEAVMSFVPVDKIAVHFHDTYGQALANILVSLQLGINIVDSSVSGLG 314

Query: 390 GCPYAKGASGNVATEDVVYMLNGLGISTNVDLGKVMAAGEFICNHLGRQSGSKAAIAL 447
           GCPYAKGA+GNVATEDVVYML+GLGI TNVDL K+M AG++I  HLGRQSGSK   AL
Sbjct: 315 GCPYAKGATGNVATEDVVYMLHGLGIETNVDLNKLMDAGDYISKHLGRQSGSKTTTAL 372



>Os11g0137200 Pyruvate carboxyltransferase domain containing protein
          Length = 68

 Score = 99.4 bits (246), Expect = 5e-21,   Method: Composition-based stats.
 Identities = 47/62 (75%), Positives = 54/62 (87%)

Query: 149 KIIHDLPRSVKIVEVGPRDGLQNEKNIVPTHVKIELIQRLATSGLSVVEATSFVSPKWVP 208
           +++ +LPR VKIVEVGPRDGLQNEK+ VP  VKIELI +L  SGLSVVEATSFVSPKWVP
Sbjct: 5   QVLWELPRFVKIVEVGPRDGLQNEKSTVPASVKIELIHKLVASGLSVVEATSFVSPKWVP 64

Query: 209 QL 210
           Q+
Sbjct: 65  QV 66



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.317    0.135    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 12,365,208
Number of extensions: 445286
Number of successful extensions: 961
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 958
Number of HSP's successfully gapped: 3
Length of query: 459
Length of database: 17,035,801
Length adjustment: 104
Effective length of query: 355
Effective length of database: 11,605,545
Effective search space: 4119968475
Effective search space used: 4119968475
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 158 (65.5 bits)