BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0207300 Os01g0207300|Os01g0207300
         (164 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0207300  Similar to S-adenosylmethionine synthetase 1 (...   341   2e-94
AK071347                                                          327   3e-90
Os05g0135700  S-adenosylmethionine synthetase 1 (EC 2.5.1.6)...   315   6e-87
Os01g0293000  Similar to S-adenosylmethionine synthetase 1 (...   312   8e-86

>Os01g0207300 Similar to S-adenosylmethionine synthetase 1 (EC 2.5.1.6)
           (Methionine adenosyltransferase 1) (AdoMet synthetase
           1). Splice isoform 2
          Length = 164

 Score =  341 bits (874), Expect = 2e-94,   Method: Compositional matrix adjust.
 Identities = 164/164 (100%), Positives = 164/164 (100%)

Query: 1   MAAETFLFTSESRNEGHPDKLCDQVSDAVLDVCLAQDPEGKVACETCTKTNMVMVFGEIT 60
           MAAETFLFTSESRNEGHPDKLCDQVSDAVLDVCLAQDPEGKVACETCTKTNMVMVFGEIT
Sbjct: 1   MAAETFLFTSESRNEGHPDKLCDQVSDAVLDVCLAQDPEGKVACETCTKTNMVMVFGEIT 60

Query: 61  TKASVDYEKIVRDTCRNIGFVSDDIGLDADRCKVLVNIEQQSPDIAQGVHGHFTKHPEEI 120
           TKASVDYEKIVRDTCRNIGFVSDDIGLDADRCKVLVNIEQQSPDIAQGVHGHFTKHPEEI
Sbjct: 61  TKASVDYEKIVRDTCRNIGFVSDDIGLDADRCKVLVNIEQQSPDIAQGVHGHFTKHPEEI 120

Query: 121 GAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCA 164
           GAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCA
Sbjct: 121 GAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCA 164



>AK071347 
          Length = 237

 Score =  327 bits (837), Expect = 3e-90,   Method: Compositional matrix adjust.
 Identities = 156/164 (95%), Positives = 159/164 (96%)

Query: 1   MAAETFLFTSESRNEGHPDKLCDQVSDAVLDVCLAQDPEGKVACETCTKTNMVMVFGEIT 60
           MAAETFLFTSES NEGHPDKLCDQVSDAVLD CLAQDP+ KVACETCTKTNMVMVFGEIT
Sbjct: 1   MAAETFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEIT 60

Query: 61  TKASVDYEKIVRDTCRNIGFVSDDIGLDADRCKVLVNIEQQSPDIAQGVHGHFTKHPEEI 120
           TKA+VDYEKIVRDTCR IGFVSDD+GLDADRCKVLVNIEQQSPDIAQGVHGHFTK PEEI
Sbjct: 61  TKATVDYEKIVRDTCRGIGFVSDDVGLDADRCKVLVNIEQQSPDIAQGVHGHFTKRPEEI 120

Query: 121 GAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCA 164
           GAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCA
Sbjct: 121 GAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCA 164



>Os05g0135700 S-adenosylmethionine synthetase 1 (EC 2.5.1.6) (Methionine
           adenosyltransferase 1) (AdoMet synthetase 1)
          Length = 396

 Score =  315 bits (808), Expect = 6e-87,   Method: Compositional matrix adjust.
 Identities = 150/163 (92%), Positives = 156/163 (95%)

Query: 2   AAETFLFTSESRNEGHPDKLCDQVSDAVLDVCLAQDPEGKVACETCTKTNMVMVFGEITT 61
           A +TFLFTSES NEGHPDKLCDQVSDAVLD CLA+DP+ KVACETCTKTNMVMVFGEITT
Sbjct: 3   ALDTFLFTSESVNEGHPDKLCDQVSDAVLDACLAEDPDSKVACETCTKTNMVMVFGEITT 62

Query: 62  KASVDYEKIVRDTCRNIGFVSDDIGLDADRCKVLVNIEQQSPDIAQGVHGHFTKHPEEIG 121
           KA+VDYEKIVR+TCRNIGFVS D+GLDAD CKVLVNIEQQSPDIAQGVHGHFTK PEEIG
Sbjct: 63  KANVDYEKIVRETCRNIGFVSADVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIG 122

Query: 122 AGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCA 164
           AGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCA
Sbjct: 123 AGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCA 165



>Os01g0293000 Similar to S-adenosylmethionine synthetase 1 (EC 2.5.1.6)
           (Methionine adenosyltransferase 1) (AdoMet synthetase 1)
           (Fragment)
          Length = 396

 Score =  312 bits (799), Expect = 8e-86,   Method: Compositional matrix adjust.
 Identities = 147/161 (91%), Positives = 154/161 (95%)

Query: 4   ETFLFTSESRNEGHPDKLCDQVSDAVLDVCLAQDPEGKVACETCTKTNMVMVFGEITTKA 63
           +TFLFTSES NEGHPDKLCDQ+SDAVLD CLA+DPE KVACETCTKTNMVMVFGEITTKA
Sbjct: 5   DTFLFTSESVNEGHPDKLCDQISDAVLDACLAEDPESKVACETCTKTNMVMVFGEITTKA 64

Query: 64  SVDYEKIVRDTCRNIGFVSDDIGLDADRCKVLVNIEQQSPDIAQGVHGHFTKHPEEIGAG 123
           +VDYEKIVRDTCR IGFVS+D+GLDA+ CKVLVNIEQQSPDIAQGVHGHFTK PEEIGAG
Sbjct: 65  NVDYEKIVRDTCRGIGFVSNDVGLDAEHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAG 124

Query: 124 DQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCA 164
           DQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNG CA
Sbjct: 125 DQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGACA 165



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.318    0.134    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,636,853
Number of extensions: 220511
Number of successful extensions: 482
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 482
Number of HSP's successfully gapped: 4
Length of query: 164
Length of database: 17,035,801
Length adjustment: 93
Effective length of query: 71
Effective length of database: 12,179,899
Effective search space: 864772829
Effective search space used: 864772829
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 152 (63.2 bits)