BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os01g0197200 Os01g0197200|J100055C06
(71 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os01g0197200 Similar to Ethylene-responsive RNA helicase 143 2e-35
AK111212 102 5e-23
AK107287 89 8e-19
Os01g0172200 WW/Rsp5/WWP domain containing protein 87 2e-18
Os01g0549400 Similar to RNA helicase-like protein DB10 86 7e-18
Os11g0689400 WW/Rsp5/WWP domain containing protein 71 2e-13
>Os01g0197200 Similar to Ethylene-responsive RNA helicase
Length = 71
Score = 143 bits (361), Expect = 2e-35, Method: Compositional matrix adjust.
Identities = 71/71 (100%), Positives = 71/71 (100%)
Query: 1 STNIFPCVIKAPGDGPIVLVLAPTRELAVQIQQEATKFGASSKIKSTCIYGGVPKGPQVR 60
STNIFPCVIKAPGDGPIVLVLAPTRELAVQIQQEATKFGASSKIKSTCIYGGVPKGPQVR
Sbjct: 1 STNIFPCVIKAPGDGPIVLVLAPTRELAVQIQQEATKFGASSKIKSTCIYGGVPKGPQVR 60
Query: 61 DLQKGSLSFLC 71
DLQKGSLSFLC
Sbjct: 61 DLQKGSLSFLC 71
>AK111212
Length = 545
Score = 102 bits (255), Expect = 5e-23, Method: Composition-based stats.
Identities = 44/55 (80%), Positives = 51/55 (92%)
Query: 11 APGDGPIVLVLAPTRELAVQIQQEATKFGASSKIKSTCIYGGVPKGPQVRDLQKG 65
APGDGPIVLVLAPTRELAVQIQ+E KFG SS+I++TC+YGGVPKGPQ+RDL +G
Sbjct: 206 APGDGPIVLVLAPTRELAVQIQEEMKKFGRSSRIRNTCVYGGVPKGPQIRDLSRG 260
>AK107287
Length = 554
Score = 88.6 bits (218), Expect = 8e-19, Method: Composition-based stats.
Identities = 37/55 (67%), Positives = 46/55 (83%)
Query: 12 PGDGPIVLVLAPTRELAVQIQQEATKFGASSKIKSTCIYGGVPKGPQVRDLQKGS 66
PGDGPI L+LAPTRELA QIQ E +FG SS++++ +YGGVPKGPQ RDLQ+G+
Sbjct: 203 PGDGPIALILAPTRELANQIQVECNRFGGSSRLRTCAVYGGVPKGPQTRDLQRGA 257
>Os01g0172200 WW/Rsp5/WWP domain containing protein
Length = 759
Score = 87.4 bits (215), Expect = 2e-18, Method: Composition-based stats.
Identities = 38/53 (71%), Positives = 45/53 (84%)
Query: 14 DGPIVLVLAPTRELAVQIQQEATKFGASSKIKSTCIYGGVPKGPQVRDLQKGS 66
DGP VLVL+PTRELA QIQ EA KFG SS+I S C+YGG PKGPQ+RDL++G+
Sbjct: 302 DGPTVLVLSPTRELATQIQDEAKKFGRSSRISSVCLYGGAPKGPQLRDLERGA 354
>Os01g0549400 Similar to RNA helicase-like protein DB10
Length = 792
Score = 85.5 bits (210), Expect = 7e-18, Method: Composition-based stats.
Identities = 39/54 (72%), Positives = 44/54 (81%)
Query: 12 PGDGPIVLVLAPTRELAVQIQQEATKFGASSKIKSTCIYGGVPKGPQVRDLQKG 65
P GP VLVLAPTRELA QI +EA KFG SS+I STC+YGG PKGPQ+RDL +G
Sbjct: 219 PRSGPTVLVLAPTRELATQILEEAVKFGRSSRISSTCLYGGAPKGPQLRDLDRG 272
>Os11g0689400 WW/Rsp5/WWP domain containing protein
Length = 1398
Score = 71.2 bits (173), Expect = 2e-13, Method: Composition-based stats.
Identities = 35/55 (63%), Positives = 43/55 (78%)
Query: 12 PGDGPIVLVLAPTRELAVQIQQEATKFGASSKIKSTCIYGGVPKGPQVRDLQKGS 66
P GP VLVLAPTRELA QIQ EA KFG SS++ TC+YGG KG Q+R+L++G+
Sbjct: 673 PMLGPTVLVLAPTRELASQIQDEAVKFGRSSRVSCTCLYGGTSKGLQLRELERGA 727
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.319 0.137 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,394,958
Number of extensions: 72161
Number of successful extensions: 169
Number of sequences better than 1.0e-10: 6
Number of HSP's gapped: 173
Number of HSP's successfully gapped: 6
Length of query: 71
Length of database: 17,035,801
Length adjustment: 42
Effective length of query: 29
Effective length of database: 14,842,813
Effective search space: 430441577
Effective search space used: 430441577
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 149 (62.0 bits)