BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0102600 Os01g0102600|AK064812
         (269 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0102600  Shikimate kinase domain containing protein          472   e-133
Os06g0225800  Shikimate kinase domain containing protein           98   7e-21
Os04g0640600  Shikimate kinase domain containing protein           91   6e-19

>Os01g0102600 Shikimate kinase domain containing protein
          Length = 269

 Score =  472 bits (1214), Expect = e-133,   Method: Compositional matrix adjust.
 Identities = 235/256 (91%), Positives = 235/256 (91%)

Query: 14  SLGPGAVSWNXXXXXXXXXXXXXXXXXXXXXAFPSSEQTLEELNPSVELLRKTAEAVGDF 73
           SLGPGAVSWN                     AFPSSEQTLEELNPSVELLRKTAEAVGDF
Sbjct: 14  SLGPGAVSWNSKRFFLSSSSTTSRPHRRRLRAFPSSEQTLEELNPSVELLRKTAEAVGDF 73

Query: 74  RKTPIYIVGTDCTAKRNIAKLLANSIIYRYLCSEELLEDVLGGKDALNAFRESDLNGYLE 133
           RKTPIYIVGTDCTAKRNIAKLLANSIIYRYLCSEELLEDVLGGKDALNAFRESDLNGYLE
Sbjct: 74  RKTPIYIVGTDCTAKRNIAKLLANSIIYRYLCSEELLEDVLGGKDALNAFRESDLNGYLE 133

Query: 134 VETEGLKQLTSMGSLVLCCGDGAVMNSTNLGLLRHGVSIWIDIPLELVVNDMLKTQATSD 193
           VETEGLKQLTSMGSLVLCCGDGAVMNSTNLGLLRHGVSIWIDIPLELVVNDMLKTQATSD
Sbjct: 134 VETEGLKQLTSMGSLVLCCGDGAVMNSTNLGLLRHGVSIWIDIPLELVVNDMLKTQATSD 193

Query: 194 PDSFSEAMSRVRQRHDELKERYGVSDITVSVQNVASQLGYSSIDSVTLEDMVLEIVRQIE 253
           PDSFSEAMSRVRQRHDELKERYGVSDITVSVQNVASQLGYSSIDSVTLEDMVLEIVRQIE
Sbjct: 194 PDSFSEAMSRVRQRHDELKERYGVSDITVSVQNVASQLGYSSIDSVTLEDMVLEIVRQIE 253

Query: 254 RLIRAKSMMEAAGKPF 269
           RLIRAKSMMEAAGKPF
Sbjct: 254 RLIRAKSMMEAAGKPF 269



>Os06g0225800 Shikimate kinase domain containing protein
          Length = 307

 Score = 97.8 bits (242), Expect = 7e-21,   Method: Compositional matrix adjust.
 Identities = 54/202 (26%), Positives = 105/202 (51%), Gaps = 7/202 (3%)

Query: 62  LLRKTAEAVGDFRKTPIYIVGTDCTAKRNIAKLLANSIIYRYLCSEELLEDVLGGKDALN 121
           L RK+ E +       IY+VG   + K  +AK+LA  + Y +  S++L+E  +G      
Sbjct: 81  LKRKSEEVLFYLNGRCIYLVGMMGSGKSTVAKILAEVLGYSFFDSDKLVEQAVGMPSVAQ 140

Query: 122 AFRESDLNGYLEVETEGLKQLTSMGSLVLCCGDGAVMNSTNLGLLRHGVSIWIDIPLELV 181
            F+E     + + E+  L+ L+SM  LV+  G GAV+   N   ++ G+S+W+D+PL+ +
Sbjct: 141 IFKEHSEAFFRDNESSVLRDLSSMRRLVVATGGGAVIRPVNWKYMKKGLSVWLDVPLDAL 200

Query: 182 VNDMLKTQATSDP-------DSFSEAMSRVRQRHDELKERYGVSDITVSVQNVASQLGYS 234
              + +    S P       D ++ A S++    ++  + Y  +D  VS++ +A++ G+ 
Sbjct: 201 ARRIAQVGTASRPLLDQPSSDPYTAAFSKLSMLAEQRGDAYANADARVSLEEIAAKQGHD 260

Query: 235 SIDSVTLEDMVLEIVRQIERLI 256
            +  +T  D+ +E + +IE  +
Sbjct: 261 DVSKLTPTDIAIEALLKIENFV 282



>Os04g0640600 Shikimate kinase domain containing protein
          Length = 287

 Score = 91.3 bits (225), Expect = 6e-19,   Method: Compositional matrix adjust.
 Identities = 53/221 (23%), Positives = 114/221 (51%), Gaps = 7/221 (3%)

Query: 50  EQTLEELNPSVELLRKTAEAVGDFRKTPIYIVGTDCTAKRNIAKLLANSIIYRYLCSEEL 109
           ++T    + ++ L +K  + V       IY+VG   + K  + K+LA  + Y +  S++L
Sbjct: 66  DKTYYSADEALVLKQKAEDVVPYLNDRCIYLVGMMGSGKTTVGKILAEVLGYSFFDSDKL 125

Query: 110 LEDVLGGKDALNAFRESDLNGYLEVETEGLKQLTSMGSLVLCCGDGAVMNSTNLGLLRHG 169
           +E  +G       F+      + + E+E L+ L+SM  LV+  G GAV+   N   ++ G
Sbjct: 126 VEKAVGISSVAEIFQLHSEAFFRDNESEVLRDLSSMHRLVVATGGGAVIRPINWSYMKKG 185

Query: 170 VSIWIDIPLELVVNDMLKTQATSDP-------DSFSEAMSRVRQRHDELKERYGVSDITV 222
            +IW+D+PL+ +   +      S P       D +++A +++    ++  + Y  +D  V
Sbjct: 186 STIWLDVPLDALARRIAAVGTASRPLLHQESGDPYAKAYAKLTALFEQRMDSYANADARV 245

Query: 223 SVQNVASQLGYSSIDSVTLEDMVLEIVRQIERLIRAKSMME 263
           S++++A + G+S++ ++T   + +E + ++E  +  K+M+ 
Sbjct: 246 SLEHIAVKQGHSNVTTLTPSAIAIEALLKMESFLTEKAMIR 286



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.316    0.132    0.370 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 7,198,350
Number of extensions: 263382
Number of successful extensions: 726
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 724
Number of HSP's successfully gapped: 3
Length of query: 269
Length of database: 17,035,801
Length adjustment: 99
Effective length of query: 170
Effective length of database: 11,866,615
Effective search space: 2017324550
Effective search space used: 2017324550
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 155 (64.3 bits)