BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0633000 Os12g0633000|J100059J24
         (71 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0633000  Carbohydrate kinase, FGGY family protein            148   8e-37
Os07g0640200  Carbohydrate kinase, FGGY family protein            100   2e-22

>Os12g0633000 Carbohydrate kinase, FGGY family protein
          Length = 71

 Score =  148 bits (374), Expect = 8e-37,   Method: Compositional matrix adjust.
 Identities = 71/71 (100%), Positives = 71/71 (100%)

Query: 1  VRAIIEGQFLSMRGHAERCGLPVPPKRIIATGGASSNQIILKTMASIFGCSVYTVQRPGI 60
          VRAIIEGQFLSMRGHAERCGLPVPPKRIIATGGASSNQIILKTMASIFGCSVYTVQRPGI
Sbjct: 1  VRAIIEGQFLSMRGHAERCGLPVPPKRIIATGGASSNQIILKTMASIFGCSVYTVQRPGI 60

Query: 61 YKTCASHMDSN 71
          YKTCASHMDSN
Sbjct: 61 YKTCASHMDSN 71



>Os07g0640200 Carbohydrate kinase, FGGY family protein
          Length = 562

 Score =  100 bits (250), Expect = 2e-22,   Method: Composition-based stats.
 Identities = 46/58 (79%), Positives = 51/58 (87%)

Query: 1   VRAIIEGQFLSMRGHAERCGLPVPPKRIIATGGASSNQIILKTMASIFGCSVYTVQRP 58
           VRAIIEGQ LSMRGHAER G+P PPKRIIATGGASSN+ IL ++A IFGC V+TVQRP
Sbjct: 424 VRAIIEGQLLSMRGHAERFGMPNPPKRIIATGGASSNERILHSIAQIFGCPVFTVQRP 481



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.323    0.136    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,619,410
Number of extensions: 79262
Number of successful extensions: 162
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 162
Number of HSP's successfully gapped: 2
Length of query: 71
Length of database: 17,035,801
Length adjustment: 42
Effective length of query: 29
Effective length of database: 14,842,813
Effective search space: 430441577
Effective search space used: 430441577
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 149 (62.0 bits)