BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0612400 Os12g0612400|AK120388
         (544 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0612400  Similar to S-locus protein 5 (Fragment)            1074   0.0  
Os03g0832900  Similar to DNAJ-like protein (Fragment)             210   2e-54

>Os12g0612400 Similar to S-locus protein 5 (Fragment)
          Length = 544

 Score = 1074 bits (2778), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 517/544 (95%), Positives = 517/544 (95%)

Query: 1   MADLGLWKQGWRWVVSQKHILTWAHMAASGGTERLAFLVDRHWPAVSRACVSSGRLALAA 60
           MADLGLWKQGWRWVVSQKHILTWAHMAASGGTERLAFLVDRHWPAVSRACVSSGRLALAA
Sbjct: 1   MADLGLWKQGWRWVVSQKHILTWAHMAASGGTERLAFLVDRHWPAVSRACVSSGRLALAA 60

Query: 61  LRQWRGCAARGILEMASLGPASVFVILWSFFVCITSPXXXXXXXXXXXXXXXVIHYMGYT 120
           LRQWRGCAARGILEMASLGPASVFVILWSFFVCITSP               VIHYMGYT
Sbjct: 61  LRQWRGCAARGILEMASLGPASVFVILWSFFVCITSPACALYALLGMGAAGAVIHYMGYT 120

Query: 121 PGLFIVGLFGILIMWMYGYFWITGMLLIAGGCMCSLKHARFVIPVLAMYAVYCVAVRVGS 180
           PGLFIVGLFGILIMWMYGYFWITGMLLIAGGCMCSLKHARFVIPVLAMYAVYCVAVRVGS
Sbjct: 121 PGLFIVGLFGILIMWMYGYFWITGMLLIAGGCMCSLKHARFVIPVLAMYAVYCVAVRVGS 180

Query: 181 LGVFLTLNLSFLTNDLLNKLLQGYEGSTEERQFEEPKHSDPVMDEFYRSCEFPSAPDSEP 240
           LGVFLTLNLSFLTNDLLNKLLQGYEGSTEERQFEEPKHSDPVMDEFYRSCEFPSAPDSEP
Sbjct: 181 LGVFLTLNLSFLTNDLLNKLLQGYEGSTEERQFEEPKHSDPVMDEFYRSCEFPSAPDSEP 240

Query: 241 ETVSSAKPFCSTPVQDVLHVQKEAXXXXXXXXXXXXLDEMKRIMDGLTHYEVLGIPRNRS 300
           ETVSSAKPFCSTPVQDVLHVQKEA            LDEMKRIMDGLTHYEVLGIPRNRS
Sbjct: 241 ETVSSAKPFCSTPVQDVLHVQKEASPSKVVKSDSVSLDEMKRIMDGLTHYEVLGIPRNRS 300

Query: 301 IDQKILKKEYHRMVLLVHPDKNMGNPLACESFKKLQSAYEVLSDFTKKNTYDDQLRKEES 360
           IDQKILKKEYHRMVLLVHPDKNMGNPLACESFKKLQSAYEVLSDFTKKNTYDDQLRKEES
Sbjct: 301 IDQKILKKEYHRMVLLVHPDKNMGNPLACESFKKLQSAYEVLSDFTKKNTYDDQLRKEES 360

Query: 361 RKMTQRSRVVSQQTGVEFLSEESRRIQCTKCGNFHLWICTKKSKAKARWCQDCSDFHPAK 420
           RKMTQRSRVVSQQTGVEFLSEESRRIQCTKCGNFHLWICTKKSKAKARWCQDCSDFHPAK
Sbjct: 361 RKMTQRSRVVSQQTGVEFLSEESRRIQCTKCGNFHLWICTKKSKAKARWCQDCSDFHPAK 420

Query: 421 DGDGWVENKFSSSFKEIPRAFVCAESKVFDVSEWATCQGMECKPNTHGPSFMVNMVGADR 480
           DGDGWVENKFSSSFKEIPRAFVCAESKVFDVSEWATCQGMECKPNTHGPSFMVNMVGADR
Sbjct: 421 DGDGWVENKFSSSFKEIPRAFVCAESKVFDVSEWATCQGMECKPNTHGPSFMVNMVGADR 480

Query: 481 MSQRSYSSRYPFSLNAEMIPEDEFELWLQQALASGVFSDSPKRRKSWSPFKLPQKGIKSW 540
           MSQRSYSSRYPFSLNAEMIPEDEFELWLQQALASGVFSDSPKRRKSWSPFKLPQKGIKSW
Sbjct: 481 MSQRSYSSRYPFSLNAEMIPEDEFELWLQQALASGVFSDSPKRRKSWSPFKLPQKGIKSW 540

Query: 541 RRSS 544
           RRSS
Sbjct: 541 RRSS 544



>Os03g0832900 Similar to DNAJ-like protein (Fragment)
          Length = 265

 Score =  210 bits (535), Expect = 2e-54,   Method: Compositional matrix adjust.
 Identities = 115/242 (47%), Positives = 146/242 (60%), Gaps = 27/242 (11%)

Query: 315 LLVHPDKNMGNPLACESFKKLQSAYEVLSDFTKKNTYDDQLRKEESRKMTQRSRVVSQQT 374
           +LVHPDKNMGN  A ++FKKLQ+AYEVL D  K+ TYDD+LR+EE     +R +  SQ+ 
Sbjct: 1   MLVHPDKNMGNDKAADAFKKLQNAYEVLLDSLKRKTYDDELRREELLNYFRRFQSASQKK 60

Query: 375 GVEFLSEE---------------SRRIQCTKCGNFHLWICTKKSKAKARWCQDCSDFHPA 419
           G   +  +               SRRI C KCG+FHLWI T ++K++ARWCQDC+DFH A
Sbjct: 61  GGSGIFRQGFSPSEGVDEGPYGLSRRIACKKCGDFHLWIYTGRAKSQARWCQDCNDFHQA 120

Query: 420 KDGDGWVENKFSSSF------KEIPRAFVCAESKVFDVSEWATCQGMECKPNTHGPSFMV 473
           KDGDGWVE  F           E+P A+VCAES +FDV+EW TCQGM C  NTH PSF V
Sbjct: 121 KDGDGWVEQSFQPVLFGLLHKPELPHAYVCAESIIFDVTEWFTCQGMRCPANTHKPSFHV 180

Query: 474 NMVGADRMSQRSYSSR-----YPFSLNAE-MIPEDEFELWLQQALASGVFSDSPKRRKSW 527
           N     + S +  +S       P  +N +  I E+EF  WLQ AL SG+F     + +  
Sbjct: 181 NASLLKQNSGKGSTSAQRGGGIPNGVNMDGGIDEEEFFEWLQNALQSGMFESFGAQNEPP 240

Query: 528 SP 529
           SP
Sbjct: 241 SP 242



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.322    0.135    0.432 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 18,695,313
Number of extensions: 746400
Number of successful extensions: 1846
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1861
Number of HSP's successfully gapped: 2
Length of query: 544
Length of database: 17,035,801
Length adjustment: 106
Effective length of query: 438
Effective length of database: 11,501,117
Effective search space: 5037489246
Effective search space used: 5037489246
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 158 (65.5 bits)