BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0515600 Os12g0515600|AK071160
         (382 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0515600  Similar to Guanylate kinase (EC 2.7.4.8)            759   0.0  
Os03g0320900  Guanylate kinase family protein                     101   7e-22

>Os12g0515600 Similar to Guanylate kinase (EC 2.7.4.8)
          Length = 382

 Score =  759 bits (1960), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 373/382 (97%), Positives = 373/382 (97%)

Query: 1   MGEEAPEFRVESVALESKDCLQNAIDIGDKTYVISRSDDPKSSITIKILDKLTQTWVVPT 60
           MGEEAPEFRVESVALESKDCLQNAIDIGDKTYVISRSDDPKSSITIKILDKLTQTWVVPT
Sbjct: 1   MGEEAPEFRVESVALESKDCLQNAIDIGDKTYVISRSDDPKSSITIKILDKLTQTWVVPT 60

Query: 61  VLGAPPNPTSSHSAVLVNNEKILIIEKGVPLNDSIWFLEVDTPFVKQQSKIKGTVVVAWS 120
           VLGAPPNPTSSHSAVLVNNEKILIIEKGVPLNDSIWFLEVDTPFVKQQSKIKGTVVVAWS
Sbjct: 61  VLGAPPNPTSSHSAVLVNNEKILIIEKGVPLNDSIWFLEVDTPFVKQQSKIKGTVVVAWS 120

Query: 121 KGVIGEGQKPIVISGPSGVGKGTLIAKLMKEYPSKFGFSVSHTTRAPREKEIDGVHYHFT 180
           KGVIGEGQKPIVISGPSGVGKGTLIAKLMKEYPSKFGFSVSHTTRAPREKEIDGVHYHFT
Sbjct: 121 KGVIGEGQKPIVISGPSGVGKGTLIAKLMKEYPSKFGFSVSHTTRAPREKEIDGVHYHFT 180

Query: 181 ERSKIEEEISEGKFLEFAHVHGNVYGTSVEAVESVTDEGKRCILDIDVQGARSVRASSLE 240
           ERSKIEEEISEGKFLEFAHVHGNVYGTSVEAVESVTDEGKRCILDIDVQGARSVRASSLE
Sbjct: 181 ERSKIEEEISEGKFLEFAHVHGNVYGTSVEAVESVTDEGKRCILDIDVQGARSVRASSLE 240

Query: 241 AIFIFVCPPSFEELEKRLRARGTETEEQIQKRLRNARAELDQSNSPGLFDHLLVNDDLEA 300
           AIFIFVCPPSFEELEKRLRARGTETEEQIQKRLRNARAELDQSNSPGLFDHLLVNDDLEA
Sbjct: 241 AIFIFVCPPSFEELEKRLRARGTETEEQIQKRLRNARAELDQSNSPGLFDHLLVNDDLEA 300

Query: 301 CYENLKKLLSLDDDHEDSNDSFIKDGKETACYSILSKTNSEILLQSETNEAEKGTTNXXX 360
           CYENLKKLLSLDDDHEDSNDSFIKDGKETACYSILSKTNSEILLQSETNEAEKGTTN   
Sbjct: 301 CYENLKKLLSLDDDHEDSNDSFIKDGKETACYSILSKTNSEILLQSETNEAEKGTTNLLS 360

Query: 361 XXXXXXGGAPGRTRGLKISPVN 382
                 GGAPGRTRGLKISPVN
Sbjct: 361 LDLSLSGGAPGRTRGLKISPVN 382



>Os03g0320900 Guanylate kinase family protein
          Length = 285

 Score =  101 bits (252), Expect = 7e-22,   Method: Compositional matrix adjust.
 Identities = 63/168 (37%), Positives = 95/168 (56%), Gaps = 5/168 (2%)

Query: 131 IVISGPSGVGKGTLIAKLMKEYPSKFGFSVSHTTRAPREKEIDGVHYHFTERSKIEEEIS 190
           +VISGPSGVGK  +I +L +E      F V+ T+RA R  E+DG  Y+F  + +    I 
Sbjct: 94  LVISGPSGVGKDAVIQRLQEEREG-MHFVVTATSRAKRPGEVDGKDYYFVTKEEFLTMIE 152

Query: 191 EGKFLEFAHVHGNVYGTSVEAVESVTDEGKRCILDIDVQGARSVRASSLE-AIFIFVCPP 249
             + LE+A V+G   G   + +     +G   +L +D+QGA ++R    E AIFIF+   
Sbjct: 153 RKELLEYALVYGEYKGIPKQQIRDYMAKGYDIVLRVDIQGAATLREILGESAIFIFLVAE 212

Query: 250 SFEELEKRLRARGTETEEQIQKRLRNARAELDQSNSPGLFDHLLVNDD 297
           S E L KRL  R TET + +  R+  AR E+ + N+   FD+++VN +
Sbjct: 213 SEEALVKRLIHRKTETSDMLLVRVATAREEVKRMNN---FDYVVVNSE 257



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.312    0.132    0.373 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 12,759,165
Number of extensions: 527185
Number of successful extensions: 1563
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1561
Number of HSP's successfully gapped: 2
Length of query: 382
Length of database: 17,035,801
Length adjustment: 103
Effective length of query: 279
Effective length of database: 11,657,759
Effective search space: 3252514761
Effective search space used: 3252514761
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 157 (65.1 bits)