BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0508200 Os12g0508200|AK069349
         (155 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0508200  Hypothetical protein                                276   6e-75
Os03g0442900  Conserved hypothetical protein                       64   3e-11

>Os12g0508200 Hypothetical protein
          Length = 155

 Score =  276 bits (705), Expect = 6e-75,   Method: Compositional matrix adjust.
 Identities = 135/155 (87%), Positives = 135/155 (87%)

Query: 1   MEKEHIKMAILKQEQTFRQQVNELHRVYRVQKQLMIEMQSISTQAQAKADNRTIPRLEMD 60
           MEKEHIKMAILKQEQTFRQQVNELHRVYRVQKQLMIEMQSISTQAQAKADNRTIPRLEMD
Sbjct: 1   MEKEHIKMAILKQEQTFRQQVNELHRVYRVQKQLMIEMQSISTQAQAKADNRTIPRLEMD 60

Query: 61  HQQWYRNSGEKKAPEFVEDFDLELTLATGAGRKQEKPXXXXXXXXXXXXXXXXXXXXQRF 120
           HQQWYRNSGEKKAPEFVEDFDLELTLATGAGRKQEKP                    QRF
Sbjct: 61  HQQWYRNSGEKKAPEFVEDFDLELTLATGAGRKQEKPSNSDSGATVSSSTSAESESEQRF 120

Query: 121 PESNVALRFQNESKRHDDQLMQSPWLYQCLSLKMA 155
           PESNVALRFQNESKRHDDQLMQSPWLYQCLSLKMA
Sbjct: 121 PESNVALRFQNESKRHDDQLMQSPWLYQCLSLKMA 155



>Os03g0442900 Conserved hypothetical protein
          Length = 191

 Score = 64.3 bits (155), Expect = 3e-11,   Method: Compositional matrix adjust.
 Identities = 59/189 (31%), Positives = 80/189 (42%), Gaps = 46/189 (24%)

Query: 2   EKEHIKMAILKQEQTFRQQVNELHRVYRVQKQLM-----IEMQSIST-----------QA 45
           EKEH+KMA+L+QE+TF+QQV ELHR+YRVQK LM     I MQ IS            +A
Sbjct: 9   EKEHMKMAMLRQEETFKQQVQELHRLYRVQKLLMDAGSAITMQGISCIPEDDYHAEENEA 68

Query: 46  QAKADNRTIPRLEMDHQQWYRNSGEKKAPEFVEDFDLELTLATG--AGRKQEKPXXXXXX 103
            +     T P  + D  Q + +         +E+ +L+LTLA G     K+E P      
Sbjct: 69  GSSRPWHTFPGSDNDKPQAHTS--------VLEESELDLTLAIGRTTTTKKEAPSSSVDS 120

Query: 104 XXXXXXXXXXXXXX----------QRFPESN----------VALRFQNESKRHDDQLMQS 143
                                    R   S+          V    Q+      D L Q 
Sbjct: 121 RTSNSSSSTESGSTNCRAVMPHRPSRLGSSSAVKVVAGGPGVGTTQQHLDLEQQDALKQP 180

Query: 144 PWLYQCLSL 152
           PWL++CL+L
Sbjct: 181 PWLHRCLNL 189



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.317    0.128    0.365 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,993,939
Number of extensions: 116349
Number of successful extensions: 336
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 334
Number of HSP's successfully gapped: 3
Length of query: 155
Length of database: 17,035,801
Length adjustment: 92
Effective length of query: 63
Effective length of database: 12,232,113
Effective search space: 770623119
Effective search space used: 770623119
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 151 (62.8 bits)