BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0508100 Os12g0508100|Os12g0508100
         (240 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0508100  Similar to Acrosin precursor (EC 3.4.21.10) (5...   375   e-104
Os01g0730800  Mpv17/PMP22 family protein                           76   2e-14
Os01g0228300  Mpv17/PMP22 family protein                           74   1e-13

>Os12g0508100 Similar to Acrosin precursor (EC 3.4.21.10) (53 kDa fucose-binding
           protein)
          Length = 240

 Score =  375 bits (963), Expect = e-104,   Method: Compositional matrix adjust.
 Identities = 192/240 (80%), Positives = 192/240 (80%)

Query: 1   MPPTGALHAGGRHLLPLRRAPGAAQQPWSHVRSHLIXXXXXXXXXXXXXXXXXXXXXXXX 60
           MPPTGALHAGGRHLLPLRRAPGAAQQPWSHVRSHLI                        
Sbjct: 1   MPPTGALHAGGRHLLPLRRAPGAAQQPWSHVRSHLISSSKRPSPSPSPPPPPPPPLPVAP 60

Query: 61  XXXAFVQTXXXXXXXXXXXXXXXXXXXXXWYLGSIEARPVLTKSVTAAAIFTVADLSSQM 120
              AFVQT                     WYLGSIEARPVLTKSVTAAAIFTVADLSSQM
Sbjct: 61  STSAFVQTAGRRSGGGAGAGAAVGSGVVAWYLGSIEARPVLTKSVTAAAIFTVADLSSQM 120

Query: 121 ITLGPEDSLDLVRTLRMASYGLLISGPSLHIWFNFVSKLLPKQDVMNTFKKMFLGQAVYG 180
           ITLGPEDSLDLVRTLRMASYGLLISGPSLHIWFNFVSKLLPKQDVMNTFKKMFLGQAVYG
Sbjct: 121 ITLGPEDSLDLVRTLRMASYGLLISGPSLHIWFNFVSKLLPKQDVMNTFKKMFLGQAVYG 180

Query: 181 PIINSVFFSYNAGLQGETIPEIMARLKRDLIPTIKSGLIYWPLCDFITFKFIPVHLQGLE 240
           PIINSVFFSYNAGLQGETIPEIMARLKRDLIPTIKSGLIYWPLCDFITFKFIPVHLQGLE
Sbjct: 181 PIINSVFFSYNAGLQGETIPEIMARLKRDLIPTIKSGLIYWPLCDFITFKFIPVHLQGLE 240



>Os01g0730800 Mpv17/PMP22 family protein
          Length = 239

 Score = 75.9 bits (185), Expect = 2e-14,   Method: Compositional matrix adjust.
 Identities = 47/152 (30%), Positives = 74/152 (48%), Gaps = 19/152 (12%)

Query: 101 LTKSVTAAAIFTVADLSSQMITL-------GPED-----------SLDLVRTLRMASYGL 142
            T++VTAA++    D  +Q+          GPE            S D +R LRMASYG 
Sbjct: 65  FTQAVTAASLTLTGDTIAQVRQRIVDRRLRGPEPDSKGLVPDLLMSHDWLRALRMASYGF 124

Query: 143 LISGPSLHIWFNFVSKLLPKQDVMNTFKKMFLGQAVYGPIINSVFFSYNAGLQGETIPEI 202
           L+ GP  H W+ F+ + +PK    N   K+ L Q   GP +  V F++N    G+ + E+
Sbjct: 125 LLYGPGSHAWYQFLDQCMPKPTFANLSTKVILNQIALGPCVIGVIFAWNNLWIGK-LSEL 183

Query: 203 MARLKRDLIPTIKSGLIYWPLCDFITFKFIPV 234
            ++ + D +PT+  G  +W     I F  +P+
Sbjct: 184 PSKYRNDALPTLLFGFRFWIPVSIINFWMVPL 215



>Os01g0228300 Mpv17/PMP22 family protein
          Length = 222

 Score = 73.6 bits (179), Expect = 1e-13,   Method: Compositional matrix adjust.
 Identities = 45/173 (26%), Positives = 77/173 (44%), Gaps = 25/173 (14%)

Query: 90  WYLGSIEARPVLTKSVTAAAIFTVADLSSQMITLG----------------PEDS----- 128
           WY   +   PV T+ V++  ++ + D+ +Q +T                  PED      
Sbjct: 7   WYQQCLATHPVRTQVVSSGILWGLGDIGAQAVTHYSAPGRPRHHQHHAKNPPEDKDKEFK 66

Query: 129 LDLVRTLRMASYGLLISGPSLHIWFNFVSKLLPKQDVMNTFK----KMFLGQAVYGPIIN 184
           +D  R    +S+G    GP  H W+ ++ + + ++    TFK    K+     ++GP+  
Sbjct: 67  IDWKRVGITSSFGFAFVGPVGHYWYEYLDRFILRRYQPKTFKFVASKVAADGLLFGPVDL 126

Query: 185 SVFFSYNAGLQGETIPEIMARLKRDLIPTIKSGLIYWPLCDFITFKFIPVHLQ 237
            +FFSY     G ++ ++   +KRD IP +  G   WP      F+FIPV  Q
Sbjct: 127 LLFFSYVGLASGRSVEQVKDDVKRDFIPALVLGGTIWPAVQIANFRFIPVRYQ 179



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.325    0.140    0.434 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,358,237
Number of extensions: 208185
Number of successful extensions: 572
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 569
Number of HSP's successfully gapped: 4
Length of query: 240
Length of database: 17,035,801
Length adjustment: 98
Effective length of query: 142
Effective length of database: 11,918,829
Effective search space: 1692473718
Effective search space used: 1692473718
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 154 (63.9 bits)