BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0451300 Os12g0451300|Os12g0451300
         (213 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0451300                                                      322   1e-88
Os12g0453500  Conserved hypothetical protein                      261   3e-70

>Os12g0451300 
          Length = 213

 Score =  322 bits (825), Expect = 1e-88,   Method: Compositional matrix adjust.
 Identities = 168/213 (78%), Positives = 168/213 (78%)

Query: 1   MSKRQAILKTIWSPLAAVLRPKNCAATSRNLIVVNXXXXXXXXXXXXDATVTRKSRSSLE 60
           MSKRQAILKTIWSPLAAVLRPKNCAATSRNLIVVN            DATVTRKSRSSLE
Sbjct: 1   MSKRQAILKTIWSPLAAVLRPKNCAATSRNLIVVNAGGEIIAGGGREDATVTRKSRSSLE 60

Query: 61  DLLKIEASTNPPETKAAADPQPADMATPAINLSKESELQIVLAGIPKNGHGGVIXXXXXX 120
           DLLKIEASTNPPETKAAADPQPADMATPAINLSKESELQIVLAGIPKNGHGGVI      
Sbjct: 61  DLLKIEASTNPPETKAAADPQPADMATPAINLSKESELQIVLAGIPKNGHGGVIAGRPPP 120

Query: 121 XXXXXXXXXXXXXXXXXXXXXXXXXXXPSALRARSRAAKMVDAAALPAKRGSYWRIAGRG 180
                                      PSALRARSRAAKMVDAAALPAKRGSYWRIAGRG
Sbjct: 121 SLGGGGGAVVVRAPTVRVKVNRLVVLVPSALRARSRAAKMVDAAALPAKRGSYWRIAGRG 180

Query: 181 RGGDKSELFYQRPIPLGRRCRVQHLEEVTLSDD 213
           RGGDKSELFYQRPIPLGRRCRVQHLEEVTLSDD
Sbjct: 181 RGGDKSELFYQRPIPLGRRCRVQHLEEVTLSDD 213



>Os12g0453500 Conserved hypothetical protein
          Length = 211

 Score =  261 bits (666), Expect = 3e-70,   Method: Compositional matrix adjust.
 Identities = 141/213 (66%), Positives = 150/213 (70%), Gaps = 2/213 (0%)

Query: 1   MSKRQAILKTIWSPLAAVLRPKNCAATSRNLIVVNXXXXXXXXXXXXDATVTRKSRSSLE 60
           MSKRQAILK+IWSPL AV R KNCAATS NL+VVN            DA VTRKSRSSLE
Sbjct: 1   MSKRQAILKSIWSPLTAVFRTKNCAATSSNLVVVNAGGEIIAGGGREDAIVTRKSRSSLE 60

Query: 61  DLLKIEASTNPPETKAAADPQPADMATPAINLSKESELQIVLAGIPKNGHGGVIXXXXXX 120
           DLLKIE+S+NP  T AA +P P  MA+P  N S ESELQ+VLAG P+N HGGVI      
Sbjct: 61  DLLKIESSSNP-GTMAADEPLPNHMASPK-NSSMESELQVVLAGYPENSHGGVIAGRPPE 118

Query: 121 XXXXXXXXXXXXXXXXXXXXXXXXXXXPSALRARSRAAKMVDAAALPAKRGSYWRIAGRG 180
                                      PSALRARSRAAKMVDAAALPAKRGSYWRIAGRG
Sbjct: 119 SLDGGGGAVVVRAQTVRVKVNRLVVLVPSALRARSRAAKMVDAAALPAKRGSYWRIAGRG 178

Query: 181 RGGDKSELFYQRPIPLGRRCRVQHLEEVTLSDD 213
           RGGDKSELFYQRPIPLGRRCRVQHLEEVTLSD+
Sbjct: 179 RGGDKSELFYQRPIPLGRRCRVQHLEEVTLSDN 211



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.315    0.129    0.371 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,007,527
Number of extensions: 209823
Number of successful extensions: 837
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 833
Number of HSP's successfully gapped: 2
Length of query: 213
Length of database: 17,035,801
Length adjustment: 97
Effective length of query: 116
Effective length of database: 11,971,043
Effective search space: 1388640988
Effective search space used: 1388640988
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 154 (63.9 bits)