BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0437800 Os12g0437800|AK063833
         (65 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0437800  Similar to MPI                                      129   4e-31
Os05g0109701                                                       90   4e-19
Os12g0548700  Similar to MPI                                       79   5e-16
Os01g0615050  Proteinase inhibitor I13, potato inhibitor I f...    69   9e-13
Os01g0615100  Similar to Substilin /chymotrypsin-like inhibi...    65   2e-11

>Os12g0437800 Similar to MPI
          Length = 65

 Score =  129 bits (325), Expect = 4e-31,   Method: Compositional matrix adjust.
 Identities = 65/65 (100%), Positives = 65/65 (100%)

Query: 1  MSSSAKTSWPEVVGLSIEEAKKVILKDKPDADIVVLPFGTAVPEDFRFNRVRIFVDTVAE 60
          MSSSAKTSWPEVVGLSIEEAKKVILKDKPDADIVVLPFGTAVPEDFRFNRVRIFVDTVAE
Sbjct: 1  MSSSAKTSWPEVVGLSIEEAKKVILKDKPDADIVVLPFGTAVPEDFRFNRVRIFVDTVAE 60

Query: 61 TPRVG 65
          TPRVG
Sbjct: 61 TPRVG 65



>Os05g0109701 
          Length = 71

 Score = 89.7 bits (221), Expect = 4e-19,   Method: Compositional matrix adjust.
 Identities = 46/65 (70%), Positives = 52/65 (80%), Gaps = 1/65 (1%)

Query: 2  SSSAKTSWPEVVGLSIEEAKKVILKDKPDADIVVLPFGTAVPEDFRFNRVRIFVD-TVAE 60
          S++AK SWPEVVG+++EEAK  ILKDKPDADIVVLP G  +  D R NRVRIF   TVAE
Sbjct: 7  SAAAKRSWPEVVGMTMEEAKAAILKDKPDADIVVLPVGAPMTRDLRPNRVRIFGSATVAE 66

Query: 61 TPRVG 65
          TPRVG
Sbjct: 67 TPRVG 71



>Os12g0548700 Similar to MPI
          Length = 68

 Score = 79.3 bits (194), Expect = 5e-16,   Method: Compositional matrix adjust.
 Identities = 51/68 (75%), Positives = 53/68 (77%), Gaps = 3/68 (4%)

Query: 1  MSSSA---KTSWPEVVGLSIEEAKKVILKDKPDADIVVLPFGTAVPEDFRFNRVRIFVDT 57
          MSS     KTSWPEVVGL  EEAKKVILKD PDADIVV+P GT V  DFR NRVRIFVDT
Sbjct: 1  MSSGGEEGKTSWPEVVGLRAEEAKKVILKDMPDADIVVVPVGTPVTMDFRPNRVRIFVDT 60

Query: 58 VAETPRVG 65
          VA TP +G
Sbjct: 61 VAGTPTIG 68



>Os01g0615050 Proteinase inhibitor I13, potato inhibitor I family protein
          Length = 62

 Score = 68.6 bits (166), Expect = 9e-13,   Method: Compositional matrix adjust.
 Identities = 34/62 (54%), Positives = 46/62 (74%)

Query: 4  SAKTSWPEVVGLSIEEAKKVILKDKPDADIVVLPFGTAVPEDFRFNRVRIFVDTVAETPR 63
          + KT WPE+V  +I+EAK+ I  D+PD  I V+P GT V ++F  NRVRI+VDTVA+TP 
Sbjct: 1  TPKTEWPELVCRTIKEAKEKIKADRPDLKIEVVPVGTIVTQEFDENRVRIWVDTVAKTPT 60

Query: 64 VG 65
          +G
Sbjct: 61 IG 62



>Os01g0615100 Similar to Substilin /chymotrypsin-like inhibitor (Proteinase
          inhibitor)
          Length = 72

 Score = 64.7 bits (156), Expect = 2e-11,   Method: Compositional matrix adjust.
 Identities = 32/64 (50%), Positives = 42/64 (65%)

Query: 2  SSSAKTSWPEVVGLSIEEAKKVILKDKPDADIVVLPFGTAVPEDFRFNRVRIFVDTVAET 61
              KT WPE+VGL+IE+AK  I  D+PD  + VLP GT +      NRV ++VDTVAE 
Sbjct: 9  CGGLKTEWPELVGLTIEQAKAKIKADRPDLQVEVLPVGTIILGVVVPNRVILWVDTVAEI 68

Query: 62 PRVG 65
          P++G
Sbjct: 69 PKIG 72



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.318    0.135    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,084,509
Number of extensions: 65507
Number of successful extensions: 198
Number of sequences better than 1.0e-10: 6
Number of HSP's gapped: 198
Number of HSP's successfully gapped: 6
Length of query: 65
Length of database: 17,035,801
Length adjustment: 37
Effective length of query: 28
Effective length of database: 15,103,883
Effective search space: 422908724
Effective search space used: 422908724
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 149 (62.0 bits)