BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0406100 Os12g0406100|AK070373
         (466 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0406100  Glycoside hydrolase, family 43 protein              834   0.0  
Os01g0879400  Glycoside hydrolase, family 43 protein              569   e-162

>Os12g0406100 Glycoside hydrolase, family 43 protein
          Length = 466

 Score =  834 bits (2155), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 407/466 (87%), Positives = 407/466 (87%)

Query: 1   MGLRSKQPKALAFRCYAASHRSLTXXXXXXXXXXXXXNFHLLIIHKEDESTSIHEINRSI 60
           MGLRSKQPKALAFRCYAASHRSLT             NFHLLIIHKEDESTSIHEINRSI
Sbjct: 1   MGLRSKQPKALAFRCYAASHRSLTLAVWSLAALVVVVNFHLLIIHKEDESTSIHEINRSI 60

Query: 61  VSXXXXXXXXKFXXXXXXXXXXXXXXXXKGEQKPPSVVDEFLDESSAVHDMFFPERNMAI 120
           VS        KF                KGEQKPPSVVDEFLDESSAVHDMFFPERNMAI
Sbjct: 61  VSELEEVEEEKFRVSPPRSRRNPRAVRRKGEQKPPSVVDEFLDESSAVHDMFFPERNMAI 120

Query: 121 DPINGGNDSMYFYYPGRVWLDTDGNPIQAHGGGVLYDEKTETYFWYGENKDGKTYKAHSK 180
           DPINGGNDSMYFYYPGRVWLDTDGNPIQAHGGGVLYDEKTETYFWYGENKDGKTYKAHSK
Sbjct: 121 DPINGGNDSMYFYYPGRVWLDTDGNPIQAHGGGVLYDEKTETYFWYGENKDGKTYKAHSK 180

Query: 181 GADRVDIVGVSCYSSKDLWTWRNEGVVLRGEKKNVTHDLHKSNVLERPKVIYNDRTGKYV 240
           GADRVDIVGVSCYSSKDLWTWRNEGVVLRGEKKNVTHDLHKSNVLERPKVIYNDRTGKYV
Sbjct: 181 GADRVDIVGVSCYSSKDLWTWRNEGVVLRGEKKNVTHDLHKSNVLERPKVIYNDRTGKYV 240

Query: 241 MWMHIDDTNYTKASVGVAISDSPTGPFSYLYSKQPHDCESRDMTIFKDDNGKAYLIYSSE 300
           MWMHIDDTNYTKASVGVAISDSPTGPFSYLYSKQPHDCESRDMTIFKDDNGKAYLIYSSE
Sbjct: 241 MWMHIDDTNYTKASVGVAISDSPTGPFSYLYSKQPHDCESRDMTIFKDDNGKAYLIYSSE 300

Query: 301 DNSELHIGQLTDDYLDVTDNMRRLLIAQHREAPALFKYEGTYYMITSGCTGWXXXXXXXX 360
           DNSELHIGQLTDDYLDVTDNMRRLLIAQHREAPALFKYEGTYYMITSGCTGW        
Sbjct: 301 DNSELHIGQLTDDYLDVTDNMRRLLIAQHREAPALFKYEGTYYMITSGCTGWAPNTALAH 360

Query: 361 XXXXIMGPWETLGNPCVGGNDIFRXXXXXXXXXXVLPIPGLSGSFIFMADRWSPSELRDS 420
               IMGPWETLGNPCVGGNDIFR          VLPIPGLSGSFIFMADRWSPSELRDS
Sbjct: 361 AATAIMGPWETLGNPCVGGNDIFRSTTFFSQSTFVLPIPGLSGSFIFMADRWSPSELRDS 420

Query: 421 RYVWLPLTVGGLPDEAADYSFMFPLWSRVSIYWHRRWRLPEGWRDS 466
           RYVWLPLTVGGLPDEAADYSFMFPLWSRVSIYWHRRWRLPEGWRDS
Sbjct: 421 RYVWLPLTVGGLPDEAADYSFMFPLWSRVSIYWHRRWRLPEGWRDS 466



>Os01g0879400 Glycoside hydrolase, family 43 protein
          Length = 472

 Score =  569 bits (1466), Expect = e-162,   Method: Compositional matrix adjust.
 Identities = 263/376 (69%), Positives = 301/376 (80%), Gaps = 1/376 (0%)

Query: 89  KGEQKPPSVVDEFLDESSAVHDMFFPERNMAIDPINGGNDSMYFYYPGRVWLDTDGNPIQ 148
           +G +K P V+D++LDESSAVH +FFP+   A++P  GGNDSMYFY PGRVWLDTDG+ IQ
Sbjct: 95  RGPRKAPKVIDQYLDESSAVHALFFPDERSAVNPTKGGNDSMYFY-PGRVWLDTDGHAIQ 153

Query: 149 AHGGGVLYDEKTETYFWYGENKDGKTYKAHSKGADRVDIVGVSCYSSKDLWTWRNEGVVL 208
           AHGGG+LYD  T  Y+WYGENKDG TY+ H K   RVDI+GVSCYSSKDLW+W NEG+VL
Sbjct: 154 AHGGGILYDHITAKYYWYGENKDGLTYQTHPKSTYRVDIIGVSCYSSKDLWSWTNEGIVL 213

Query: 209 RGEKKNVTHDLHKSNVLERPKVIYNDRTGKYVMWMHIDDTNYTKASVGVAISDSPTGPFS 268
            GE  N THDLHKS VLERPKVIYND TG+YVMWMHIDD NYTKASVGVA+S+SPTGPF+
Sbjct: 214 PGEPTNFTHDLHKSKVLERPKVIYNDHTGQYVMWMHIDDANYTKASVGVAVSNSPTGPFT 273

Query: 269 YLYSKQPHDCESRDMTIFKDDNGKAYLIYSSEDNSELHIGQLTDDYLDVTDNMRRLLIAQ 328
           YLYS +PH  ESRDMTIFKDD+G AYL YSS DN+ELH+  LT DYL++T  MRR+LI +
Sbjct: 274 YLYSFRPHGFESRDMTIFKDDDGSAYLFYSSRDNTELHVSPLTKDYLNITVAMRRILIRR 333

Query: 329 HREAPALFKYEGTYYMITSGCTGWXXXXXXXXXXXXIMGPWETLGNPCVGGNDIFRXXXX 388
           HREAPA+FK +GTYYMITSGC+GW            IMGPWETLGNPCVGGN  FR    
Sbjct: 334 HREAPAVFKLQGTYYMITSGCSGWAPNRALAHAAESIMGPWETLGNPCVGGNRFFRLTTF 393

Query: 389 XXXXXXVLPIPGLSGSFIFMADRWSPSELRDSRYVWLPLTVGGLPDEAADYSFMFPLWSR 448
                 VLP+PGL G+FIFMADRW+PS L+DSRYVWLPL +GGL DE  DYSF FP WSR
Sbjct: 394 LSQSTFVLPLPGLPGTFIFMADRWNPSNLKDSRYVWLPLFIGGLADEPLDYSFGFPAWSR 453

Query: 449 VSIYWHRRWRLPEGWR 464
           VSIYWHR+WRLPE W+
Sbjct: 454 VSIYWHRKWRLPESWK 469



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.137    0.441 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 15,958,682
Number of extensions: 679827
Number of successful extensions: 1247
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1245
Number of HSP's successfully gapped: 2
Length of query: 466
Length of database: 17,035,801
Length adjustment: 104
Effective length of query: 362
Effective length of database: 11,605,545
Effective search space: 4201207290
Effective search space used: 4201207290
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 158 (65.5 bits)