BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0406000 Os12g0406000|AK065509
         (91 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0406000  Similar to Protein kinase (EC 2.7.1.37)             177   1e-45
Os03g0128700  Calcium-dependent protein kinase, isoform 11 (...    74   3e-14

>Os12g0406000 Similar to Protein kinase (EC 2.7.1.37)
          Length = 91

 Score =  177 bits (450), Expect = 1e-45,   Method: Compositional matrix adjust.
 Identities = 91/91 (100%), Positives = 91/91 (100%)

Query: 1  WSAAISGISGVKFEIQYISGIAKGIFDAVLKGHIDFQSVLKISDSAKDLIEKKKLSHCSS 60
          WSAAISGISGVKFEIQYISGIAKGIFDAVLKGHIDFQSVLKISDSAKDLIEKKKLSHCSS
Sbjct: 1  WSAAISGISGVKFEIQYISGIAKGIFDAVLKGHIDFQSVLKISDSAKDLIEKKKLSHCSS 60

Query: 61 ERLKDHEVLSIFDIFLHLNLLALCFIACLYS 91
          ERLKDHEVLSIFDIFLHLNLLALCFIACLYS
Sbjct: 61 ERLKDHEVLSIFDIFLHLNLLALCFIACLYS 91



>Os03g0128700 Calcium-dependent protein kinase, isoform 11 (EC 2.7.1.-) (CDPK 11)
          Length = 542

 Score = 73.6 bits (179), Expect = 3e-14,   Method: Composition-based stats.
 Identities = 46/77 (59%), Positives = 50/77 (64%), Gaps = 15/77 (19%)

Query: 1   WSAAI------SGISGVKFEIQYISGIAKGIFDAVLKGHIDFQS--VLKISDSAKDLIEK 52
           WSA +      SG+     E Q      +GIFDAVLKGHIDFQS    KISDSAKDLI +
Sbjct: 263 WSAGVILYVLLSGVPPFWAETQ------QGIFDAVLKGHIDFQSDPWPKISDSAKDLI-R 315

Query: 53  KKLSHCSSERLKDHEVL 69
           K LSHC SERLK HEVL
Sbjct: 316 KMLSHCPSERLKAHEVL 332



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.326    0.140    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,562,921
Number of extensions: 79997
Number of successful extensions: 341
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 339
Number of HSP's successfully gapped: 2
Length of query: 91
Length of database: 17,035,801
Length adjustment: 61
Effective length of query: 30
Effective length of database: 13,850,747
Effective search space: 415522410
Effective search space used: 415522410
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 149 (62.0 bits)