BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os12g0204100 Os12g0204100|J100026O21
(57 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os12g0204100 Major intrinsic protein family protein 114 1e-26
Os08g0152100 86 7e-18
Os08g0152000 84 3e-17
Os10g0513200 Similar to Nodulin-26 (N-26) 68 1e-12
Os08g0151900 67 4e-12
>Os12g0204100 Major intrinsic protein family protein
Length = 57
Score = 114 bits (286), Expect = 1e-26, Method: Compositional matrix adjust.
Identities = 57/57 (100%), Positives = 57/57 (100%)
Query: 1 FLSSILGDRPSTGASMNPARTLGTAIVAGNYTQIWVYMVSTPLGAIAGTGAYFAIKL 57
FLSSILGDRPSTGASMNPARTLGTAIVAGNYTQIWVYMVSTPLGAIAGTGAYFAIKL
Sbjct: 1 FLSSILGDRPSTGASMNPARTLGTAIVAGNYTQIWVYMVSTPLGAIAGTGAYFAIKL 57
>Os08g0152100
Length = 278
Score = 85.9 bits (211), Expect = 7e-18, Method: Compositional matrix adjust.
Identities = 42/56 (75%), Positives = 47/56 (83%)
Query: 2 LSSILGDRPSTGASMNPARTLGTAIVAGNYTQIWVYMVSTPLGAIAGTGAYFAIKL 57
+ +IL PSTGASMNPART+G AI G YTQIWVY+V+TPLGAIAGTGAY AIKL
Sbjct: 223 MMNILVAGPSTGASMNPARTIGAAIATGRYTQIWVYLVATPLGAIAGTGAYVAIKL 278
>Os08g0152000
Length = 305
Score = 83.6 bits (205), Expect = 3e-17, Method: Compositional matrix adjust.
Identities = 39/48 (81%), Positives = 42/48 (87%)
Query: 10 PSTGASMNPARTLGTAIVAGNYTQIWVYMVSTPLGAIAGTGAYFAIKL 57
PSTGASMNPARTLG AI G YTQIWVY+V+TPLGA+AG G YFAIKL
Sbjct: 258 PSTGASMNPARTLGPAIATGRYTQIWVYLVATPLGAVAGEGFYFAIKL 305
>Os10g0513200 Similar to Nodulin-26 (N-26)
Length = 152
Score = 68.2 bits (165), Expect = 1e-12, Method: Compositional matrix adjust.
Identities = 31/54 (57%), Positives = 41/54 (75%)
Query: 4 SILGDRPSTGASMNPARTLGTAIVAGNYTQIWVYMVSTPLGAIAGTGAYFAIKL 57
+IL P+TG SMNP RTLG A+ AGNY Q+W+Y+++ LGA+AG G Y A+KL
Sbjct: 83 NILIAGPTTGGSMNPVRTLGPAVAAGNYRQLWIYLIAPTLGAVAGAGVYTAVKL 136
>Os08g0151900
Length = 285
Score = 66.6 bits (161), Expect = 4e-12, Method: Compositional matrix adjust.
Identities = 29/47 (61%), Positives = 35/47 (74%)
Query: 10 PSTGASMNPARTLGTAIVAGNYTQIWVYMVSTPLGAIAGTGAYFAIK 56
PSTG SMNPART+G A+ G Y Q+W+Y+V+ PLGAIAG Y IK
Sbjct: 238 PSTGPSMNPARTIGAAVATGEYRQMWIYLVAPPLGAIAGAATYTLIK 284
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.320 0.134 0.399
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 1,921,764
Number of extensions: 55355
Number of successful extensions: 145
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 145
Number of HSP's successfully gapped: 5
Length of query: 57
Length of database: 17,035,801
Length adjustment: 30
Effective length of query: 27
Effective length of database: 15,469,381
Effective search space: 417673287
Effective search space used: 417673287
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 149 (62.0 bits)