BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0189300 Os12g0189300|AK068710
         (328 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0189300  Isocitrate lyase and phosphorylmutase family p...   515   e-146
Os04g0386600  Isocitrate lyase and phosphorylmutase family p...   155   4e-38

>Os12g0189300 Isocitrate lyase and phosphorylmutase family protein
          Length = 328

 Score =  515 bits (1326), Expect = e-146,   Method: Compositional matrix adjust.
 Identities = 249/265 (93%), Positives = 249/265 (93%)

Query: 27  IVQKTGFYAGFISGYAVSGSFLGTPDVGLLTPPEMAEVARRICASAPNTLFIADADTGGG 86
           IVQKTGFYAGFISGYAVSGSFLGTPDVGLLTPPEMAEVARRICASAPNTLFIADADTGGG
Sbjct: 27  IVQKTGFYAGFISGYAVSGSFLGTPDVGLLTPPEMAEVARRICASAPNTLFIADADTGGG 86

Query: 87  NALNVKRTVQDLMAAGAAGCFLEDQAWPKKCGHMHGKQVIPAEEHAVKIAAAREVVGDRD 146
           NALNVKRTVQDLMAAGAAGCFLEDQAWPKKCGHMHGKQVIPAEEHAVKIAAAREVVGDRD
Sbjct: 87  NALNVKRTVQDLMAAGAAGCFLEDQAWPKKCGHMHGKQVIPAEEHAVKIAAAREVVGDRD 146

Query: 147 FFIVARTDARSVTGLDDAIRRANLYIDAGADACFVEAPRSDEELMEICRRTKGYRVCNML 206
           FFIVARTDARSVTGLDDAIRRANLYIDAGADACFVEAPRSDEELMEICRRTKGYRVCNML
Sbjct: 147 FFIVARTDARSVTGLDDAIRRANLYIDAGADACFVEAPRSDEELMEICRRTKGYRVCNML 206

Query: 207 EGGKTPLHTRQELMEMGFHLIKSPLTTVYXXXXXXXXXXXXXXXXETTRDELHRLTTFTE 266
           EGGKTPLHTRQELMEMGFHLIKSPLTTVY                ETTRDELHRLTTFTE
Sbjct: 207 EGGKTPLHTRQELMEMGFHLIKSPLTTVYAAARALVDVLAALKRAETTRDELHRLTTFTE 266

Query: 267 FNNLVGLDSWLDIEVRFSVNSSSSV 291
           FNNLVGLDSWLDIEVRFSVNSSSSV
Sbjct: 267 FNNLVGLDSWLDIEVRFSVNSSSSV 291



>Os04g0386600 Isocitrate lyase and phosphorylmutase family protein
          Length = 389

 Score =  155 bits (392), Expect = 4e-38,   Method: Compositional matrix adjust.
 Identities = 95/263 (36%), Positives = 142/263 (53%), Gaps = 9/263 (3%)

Query: 27  IVQKTGFYAGFISGYAVSGSFLGTPDVGLLTPPEMAEVARRICASAPNTLFIADADTGGG 86
           ++Q+ GF   F+ G+ VS + LG PD GL++  EM +  R I   A +   I D D G G
Sbjct: 48  LIQRAGFPICFMGGFCVSAARLGLPDAGLISYGEMVDQGRLIT-EAVSLPVIGDGDNGYG 106

Query: 87  NALNVKRTVQDLMAAGAAGCFLEDQAWPKKCGHMHGKQVIPAEEHAVKIAA---AREVVG 143
           NA+++KRTV+  + AG AG  LEDQ  PK CGH  G++VI  E+  + I A   AR+  G
Sbjct: 107 NAMSIKRTVKGYINAGFAGIMLEDQVAPKACGHTEGRKVISREDAIMHIKAAVDARKESG 166

Query: 144 DRDFFIVARTDARSVTGLDDAIRRANLYIDAGADACFVEAPRSDEELMEICRRT-KGYRV 202
             D  I+AR+D+R    +D+A+ R   + DAGAD  F++A  S EE+   C  + K  ++
Sbjct: 167 S-DIVIIARSDSRQAISIDEALWRVQAFADAGADVLFIDALASIEEMKAFCAVSPKVPKM 225

Query: 203 CNMLE-GGKTPLHTRQELMEMGFHLIKSPLTTVYXXXXXXXXXXXXXXXXETTRDELHRL 261
            NMLE GGKTP+ +  EL E+GF L+  PL+ +                    R     L
Sbjct: 226 ANMLEGGGKTPILSPAELQEIGFSLVVYPLSLIGVSMLAMEDALIAIKSTGAPRP--GSL 283

Query: 262 TTFTEFNNLVGLDSWLDIEVRFS 284
            +F E  + +G + +   E +++
Sbjct: 284 PSFQEIKDTLGFNRYYKEEKQYA 306



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.135    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 9,023,609
Number of extensions: 357071
Number of successful extensions: 821
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 820
Number of HSP's successfully gapped: 2
Length of query: 328
Length of database: 17,035,801
Length adjustment: 101
Effective length of query: 227
Effective length of database: 11,762,187
Effective search space: 2670016449
Effective search space used: 2670016449
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 156 (64.7 bits)